Molecular characterization of respiratory syncytial virus during 2015–2016 season in Portugal

Cristóvão, Paula; Pereira, Diogo Fonseca Soares; Pechirra, Pedro; Pereira‐Vaz, João; Correia, Lurdes; Rodrigues, Fernando; Andrade, Graça; Côrte‐Real, Rita; Branquinho, Paula; Peres, Maria João; Viseu, Regina; Balseiro, Maria Jesus; Mota, Paula; Guiomar, Raquel

doi:10.1016/j.jcv.2016.08.244

Cited by 2 publications

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“…Regarding RSV-B genetic diversity, all belonged to BA genotype, which has already been detected in at least 23 countries [50]. BA9 genotype has predominated in Portugal since 2010 [27,42] as in other countries [10,45,49,51], being detected alongside with BA10 genotype until 2015/16, when BA9 remained the only genotype detected onwards. Since 2001, new genotypes have been added over time by different authors following the proposed criteria by Venter et al [5].…”

Section: Discussionmentioning

confidence: 94%

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Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014–2018

Sáez-López

Cristóvão

Costa

et al. 2019

Journal of Clinical Virology

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A B S T R A C TIntroduction: Respiratory syncytial virus (RSV) is associated with substantial morbidity and mortality since it is a predominant viral agent causing respiratory tract infections in infants, young children and the elderly. Considering the availability of the RSV vaccines in the coming years, molecular understanding in RSV is necessary. Objective: The objective of the present study was to describe RSV epidemiology and genotype variability in Portugal during the 2014/15-2017/18 period. Material and methods: Epidemiological data and RSV-positive samples from patients with a respiratory infection were collected through the non-sentinel and sentinel influenza surveillance system (ISS). RSV detection, subtyping in A and B, and sequencing of the second hypervariable region (HVR2) of G gene were performed by molecular methods. Phylogenetic trees were generated using the Neighbor-Joining method and p-distance model on MEGA 7.0. Results: RSV prevalence varied between the sentinel (2.5%, 97/3891) and the non-sentinel ISS (20.7%, 3138/ 16779), being higher (P < 0.0001) among children aged < 5 years. Bronchiolitis (62.9%, 183/291) and influenza-like illness (24.6%, 14/57) were associated (P < 0.0001) with RSV laboratory confirmation among children aged < 6 months and adults ≥65 years, respectively. The HVR2 was sequenced for 562 samples. RSV-A (46.4%, 261/562) and RSV-B (53.6%, 301/562) strains clustered mainly to ON1 (89.2%, 233/261) and BA9

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Section: Discussionmentioning

confidence: 94%

“…RSV-A and RSV-B subtypes co-circulated between 2010 and 2018 in Portugal [27,42]. RSV-A subtype was the most prevalent in the 2015/ 16 season, whereas RSV-B subtype dominated in the next two consecutive seasons (2016/17 and 2017/2018).…”

Section: Discussionmentioning

confidence: 99%