Molecular characterization of relatedness among colour variants of Asian Arowana (Scleropages formosus)

Mohd-Shamsudin, Maizatul Izzah; Fard, Maryam Zahedi; Mather, Peter B.; Suleiman, Zaini; Hassan, Ruhana; Othman, Rofina Yasmin; Bhassu, Subha

doi:10.1016/j.gene.2011.08.025

Cited by 18 publications

(18 citation statements)

References 50 publications

(54 reference statements)

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“…Thus, the study of the divergence among extant bony tongues represents a unique opportunity to elucidate the evolutionary dynamics of these old fishes. The previous studies showed the inter-and intra-species relationships of Osteoglossidae using different genetic markers, such as simple sequence repeats (SSRs) (Yue et al 2004;Mu et al 2011) and mitochondrial DNA (Kumazawa & Mutsumi 2000;Inoue et al 2001;Mohd-Shamsudin et al 2011;Mu et al 2012). To better reconsider the phylogenetic relationships among the family Osteoglossidae and to estimate the divergence time of the genus Osteoglossum, we newly amplified by polymerase chain reaction (PCR) and sequenced the mitochondrial genomes of O. ferreirai Kanazawa, 1966, belonging to the genus Osteoglossum.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the mitochondrial genome and phylogeny of the black arowana (Osteoglossum ferreirai)

Liu

Wang

et al. 2014

Biologia

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In this study, we sequenced and assembled the mitochondrial (mt) genome of Osteoglossum ferreirai to re-assess the phylogenetic relationship of the family Osteoglossidae. We determined that the mitogenome of O. ferreirai contains the entire set of 37 mt genes, and the nucleotide composition and gene arrangement were similar to those of other bonytongues. Our phylogenetic analyses exhibited monophyly of the family Osteoglossidae with high bootstrap support, which is in agreement with the currently accepted phylogenetic viewpoint that is based on both morphological and molecular approaches. These findings provide additional informative data for the further study of phylogenetic relationships and help to elucidate a key component of the species radiation process within the family Osteoglossidae.

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Section: Introductionmentioning

confidence: 99%

Characterization of the mitochondrial genome and phylogeny of the black arowana (Osteoglossum ferreirai)

Liu

Wang

et al. 2014

Biologia

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“…With the glory as an attractive species identifier in fish biodiversity research [19], the application of DNA barcoding has recently reflected 28% increase of North American freshwater fish diversity [20]. The technique further bears application in monitoring fish products for health safety [21], [22] and in regulating the exploitation of fish species under aquarium trade [23], [24], [25].…”

Section: Introductionmentioning

confidence: 99%

Identification and Re-Evaluation of Freshwater Catfishes through DNA Barcoding

et al. 2012

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BackgroundCatfishes are globally demanded as human food, angling sport and aquariums keeping thus are highly exploited all over the world. North-East India possess high abundance of catfishes and are equally exploited through decades. The strategies for conservation necessitate understanding the actual species composition, which is hampered due to sporadic descriptions of the species through traditional taxonomy. Therefore, actual catfish diversity in this region is important to be studied through the combined approach of morphological and molecular technique of DNA barcoding.Methodology/Principal FindingsAltogether 75 native catfish specimens were collected from across the North-East India and their morphological features were compared with the taxonomic keys. The detailed taxonomic study identified 25 species belonging to 17 genera and 9 families. The cytochrome oxidase c subunit-I gene fragment were then sequenced from the samples in accordance with the standard DNA barcoding protocols. The sequences were compared with public databases, viz., GenBank and BOLD. Sequences developed in the current study and from databases of the same and related taxa were analyzed to calculate the congeneric and conspecific genetic divergences using Kimura 2-parameter distance model, and a Neighbor Joining tree was created using software MEGA5.1. The DNA barcoding approach delineated 21 distinct species showing 4.33 folds of difference between the nearest congeners. Four species, viz., Amblyceps apangi, Glyptothorax telchitta, G. trilineatus and Erethistes pusillus, showed high conspecific divergence; hence their identification through molecular approach remained inconclusive. On the other hand, the database sequences for three species, viz., Mystus horai, Bagarius yarrelli and Clarias batrachus, appeared mislabeled.ConclusionThe efficiency of DNA barcoding was reaffirmed from its success by easily identifying the major share (84%) of the studied catfish into 21 distinct species. The study contributed 27 new barcodes for 7 species and confirmed the range expansion of 2 important species in NE India.

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“…Further, because they are not easy to rear, few studies of S. formosus have been reported. At present, research on S. formosus has focused mainly on interspecific genetic variations, evolutionary status, and karyotype (Austin, Tan, Croft, Hammer, & Gan, ; Bian et al, ; Mohdshamsudin et al, ; Mu et al, ; Rahman, Zakariaismail, Tang, & Muniandy, ; Tian, Mu, Wang, Gu, & Hu, ). So far, a method to effectively identify the sex of S. formosus has not been discovered.…”

Section: Introductionmentioning

confidence: 99%

Tissue expression and bioinformatics analysis of the vitellogenin gene of Asian arowana ( Scleropages formosus )

et al. 2019

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The RACE technique was used to clone the full‐length vitellogenin (VTG) cDNA sequence of Asian arowana (Scleropages formosus). The full‐length sequence was 5,550 bp with an open reading frame of 5,238 bp, encoding 1,745 amino acids, and 5′ and 3′ UTRs (untranslated regions) of 45 bp and 267 bp, respectively. Phylogenetic analysis showed that S. formosus and silver arowana (Osteoglossum bicirrhosum) share a close evolutionary relationship (bootstrap 100%). The quantitative real‐time PCR results showed that vtg expression was significantly higher in liver and gonads of male and female fish compared with its expression in the other tissues tested (p < 0.01). The relative expression levels of vtg in liver, gland, kidney, heart, head kidney, and brain of female fish were significantly higher than in the corresponding tissues of male fish (p < 0.05).

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Molecular characterization of relatedness among colour variants of Asian Arowana (Scleropages formosus)

Cited by 18 publications

References 50 publications

Characterization of the mitochondrial genome and phylogeny of the black arowana (Osteoglossum ferreirai)

Characterization of the mitochondrial genome and phylogeny of the black arowana (Osteoglossum ferreirai)

Identification and Re-Evaluation of Freshwater Catfishes through DNA Barcoding

Tissue expression and bioinformatics analysis of the vitellogenin gene of Asian arowana ( Scleropages formosus )

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