Molecular characterization of RAPD and SCAR markers linked to the Tm-1 locus in tomato

Ohmori, T.; Masayuki, Murata; Motoyoshi, Fusao

doi:10.1007/s001220050107

Cited by 26 publications

(26 citation statements)

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“…These results suggest that the Tm-1 gene operates after virus uncoating at an early stage of RNA replication. TheTm-1 locus has been mapped to the short arm of chromosome 2, close to a ribosomal RNA gene (Ohmori et al 1996).…”

Section: Host Proteins Involved In Tmv Rna Replicationmentioning

confidence: 99%

Replication of tobacco mosaic virus RNA

Buck

1999

Phil. Trans. R. Soc. Lond. B

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The replication of tobacco mosaic virus (TMV) RNA involves synthesis of a negative-strand RNA using the genomic positive-strand RNA as a template, followed by the synthesis of positive-strand RNA on the negative-strand RNA templates. Intermediates of replication isolated from infected cells include completely double-stranded RNA (replicative form) and partly double-stranded and partly single-stranded RNA (replicative intermediate), but it is not known whether these structures are double-stranded or largely single-stranded in vivo. The synthesis of negative strands ceases before that of positive strands, and positive and negative strands may be synthesized by two di¡erent polymerases. The genomic-length negative strand also serves as a template for the synthesis of subgenomic mRNAs for the virus movement and coat proteins. Both the virus-encoded 126-kDa protein, which has amino-acid sequence motifs typical of methyltransferases and helicases, and the 183-kDa protein, which has additional motifs characteristic of RNA-dependent RNA polymerases, are required for e¤cient TMV RNA replication. Puri¢ed TMV RNA polymerase also contains a host protein serologically related to the RNA-binding subunit of the yeast translational initiation factor, eIF3. Study of Arabidopsis mutants defective in RNA replication indicates that at least two host proteins are needed for TMV RNA replication. The tomato resistance gene Tm-1 may also encode a mutant form of a host protein component of the TMV replicase. TMV replicase complexes are located on the endoplasmic reticulum in close association with the cytoskeleton in cytoplasmic bodies called viroplasms, which mature to produce`X bodies'. Viroplasms are sites of both RNA replication and protein synthesis, and may provide compartments in which the various stages of the virus mutiplication cycle (protein synthesis, RNA replication, virus movement, encapsidation) are localized and coordinated. Membranes may also be important for the con¢guration of the replicase with respect to initiation of RNA synthesis, and synthesis and release of progeny single-stranded RNA.

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Section: Host Proteins Involved In Tmv Rna Replicationmentioning

confidence: 99%

Replication of tobacco mosaic virus RNA

Buck

1999

Phil. Trans. R. Soc. Lond. B

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“…Nucleic acid extractions from leaf tissues were performed as described by Ohmori et al (1996). Poly(A) + RNA was purified from total RNAs by two passages through an oligo(dT)-cellulose spin column (Amersham Pharmacia Biotech).…”

Section: Nucleic Acid Extractions and Gel Blot Analysismentioning

confidence: 99%

The Purple leaf (Pl) Locus of Rice: the Plw Allele has a Complex Organization and Includes Two Genes Encoding Basic Helix-Loop-Helix Proteins Involved in Anthocyanin Biosynthesis

Sakamoto

Ohmori

Kageyama

et al. 2001

Plant and Cell Physiology

117

115

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;The Purple leaf (Pl) locus of rice (Oryza sativa L.) affects regulation of anthocyanin biosynthesis in various plant tissues. The tissue-specific patterns of anthocyanin pigmentation, together with the syntenic relationship, indicate that the rice Pl locus may play a role in the anthocyanin pathway similar to the maize R/B loci. We isolated two cDNAs showing significant identity to the basic helix-loophelix (bHLH) proteins found in the maize R gene family. OSB1 appeared to be allelic to the previously isolated R homologue, Ra1, but showed a striking difference at the Cterminus because of a 2-bp deletion. Characterization of the corresponding genomic region revealed that the sequence identical to a 5¢ ¢ ¢ ¢-portion of OSB2 existed~10-kb downstream of the OSB1 coding region. OSB2 lacks a conserved C-terminal domain. Restriction fragment length polymorphism analyses using an F 2 population indicate that both genes co-segregate with the purple leaf phenotype. A transient complementation assay showed that the anthocyanin pathway is inducible by OSB1 or OSB2. These results suggest that the Pl w allele may be complex and composed of at least two genes encoding bHLH proteins.

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“…SCAR marker transformation has been applied by researchers at home and abroad. At Abroad, Paran and Michelmore (1993) found reliable markers linked to downy mildew resistance genes in lettuce; Garcia et al (1996) found RAPD, SCAR and RFLP markers tightly linked to nematode resistance genes; Ohmori et al (1996) found SCAR markers linked to the Tm-1 locus in tomato; Melotto et al (1996) found a SCAR marker linked to I gene in common bean. In China, Liu et al(1999) used SCAR marker-assisted selection to select a Pm21 gene conferring resistance to powdery mildew in wheat; Wang et al(2000) developed PCR-based markers linked to the fertility restorer gene for the polima cytoplasmic male sterility in rapeseed (Brassica napus L.).…”

Section: Discussionmentioning

confidence: 98%

Sequence characterized amplified region markers tightly linked to the dwarf mosaic resistance gene mdm1 (t) in maize (Zea mays L.)

Sun

Zhang

et al. 2010

Euphytica

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Maize dwarf mosaic is one of the devastating and wide spread viral diseases in the world. The present investigation was carried out to develop DNA markers closely linked to the resistance gene mdm1 (t). Linkage between the markers and phenotypes was confirmed by analyzing an F 2 population obtained from a cross between a resistant parent 'Huangzaosi' and a susceptible parent 'Mo17(478)'. Four AFLP markers were found in the maize dwarf mosaic resistant plants. By using (BSA) bulked segregant analysis, two of the four AFLP markers were transformed into Sequence-characterized amplified regions markers (SCARs), nominated Rsun-1 and Rsun-2. The two amplified fragment length polymorphism (AFLP) markers, RHC-1and RHC-2, from the amplification products of primer combination E-AGC/M-CAA and E-AGC/M-GAA, showed linkage with the mdm1 (t) gene in a genetic distance 1.6 and 2.0 cM, respectively. The results indicate that the new SCAR markers will be valuable to distinguish resistant plants from susceptible plants in plantlets growing in seedbeds. The markers developed in this study are suitable for marker-assisted selection for maize dwarf mosaic resistance.

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Molecular characterization of RAPD and SCAR markers linked to the Tm-1 locus in tomato

Cited by 26 publications

References 0 publications

Replication of tobacco mosaic virus RNA

Replication of tobacco mosaic virus RNA

The Purple leaf (Pl) Locus of Rice: the Plw Allele has a Complex Organization and Includes Two Genes Encoding Basic Helix-Loop-Helix Proteins Involved in Anthocyanin Biosynthesis

Sequence characterized amplified region markers tightly linked to the dwarf mosaic resistance gene mdm1 (t) in maize (Zea mays L.)

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