Molecular characterization of Pseudomonas aeruginosa isolates from Sudanese patients: A cross-sectional study

Amoon, Reem H.; Abdallha, Amna H.; Sharif, Ahmed Osman; Moglad, Ehssan; Altyb, Hisham N.; Elzaki, Salaheldein G.; Salih, Mohamed Ahmed

doi:10.12688/f1000research.15316.1

Cited by 5 publications

(7 citation statements)

References 18 publications

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“…It can be used for reproducible and reliable bacterial identification, and also to define the species and for useful distinction between the bacterial strains. The same results obtained by [58][59]. transcribed spacer (ITS) region of the rRNA gene was selected as a target sequence because it is the most reliable gene for iden differentiation between Candida species Silver nanoparticles have been employed in the medical industry, especially for wound dressings, heart valves, medical face masks, bandages, and water filtration systems [61 biosynthesis of new nanostructured materials with high adsorption has been attracting significant interest lately [64][65][66].…”

Section: Discussionmentioning

confidence: 99%

Disinfection of Tank Water Using Biogenic Silver Nanosilica as Anti-Microbial Agents in Jeddah City

Abdelkader

Alhazmi

2021

JPRI

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The need for clean water is becoming urgent all over the world. The risk of microbial contamination of drinking water and its growing ability under poor nutritional conditions occur frequently and existing water management systems do not efficiently eliminate these micropollutants. The major goal of this research is to analyze and test the drinking water quality at random locations in Jeddah City and to investigate the integration of polyurethane foam coated nano-silica silver nanoparticles (NSAgNPs) into the water purification system. The study was conducted in Jeddah city at random intervals during August to December 2020. Twenty-five samples (n = 25) of drinking water (tank water) were collected from different locations in Jeddah. The water samples were found contaminated with E. coli, Klebsiella pneumoniae. and the most prevalent yeast species was Candida albicans. The multi-drug resistance isolates were positively identified by PCR amplification of 16S rDNA gene fragments. The antimicrobial activity of nano-silica silver nanoparticles (NSAgNP) was analyzed and their optimal concentrations that remove potentially harmful microbes were determined. The cytotoxicity of NSAgNPs by Sulforhodamine B colorimetric (SRB) assay against breast adenocarcinoma cell line (MCF-7) showed no cytotoxic effect at concentrations ≤100 µg/mL. The 50% growth inhibition concentration (IC 50) of NSAgNPs after 72 h was 35.8 µg/mL. The excellent performance of NSAgNPs can be attributed to the capping surface functional groups and smaller particle size 8.931 nm, as verified by TEM. In conclusion, the purified water obtained by using 100 mg/L NSAgNPs is free from microbial contaminants and completely safe to drink. We recommend the integration of biosynthesized foam coated silver nanoparticles with water purification system to achieve the desired final water quality.

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Section: Discussionmentioning

confidence: 99%

Disinfection of Tank Water Using Biogenic Silver Nanosilica as Anti-Microbial Agents in Jeddah City

Abdelkader

Alhazmi

2021

JPRI

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“…All bacterial genomic DNA were used as templates for PCR amplification of the 16S rDNA gene for the confirmation of P. aeruginosa. The two primers used were 27F (5′ AGAGTTTGATCCTGGCTCAG-3ʹ) and 1495R (5′ CTACGGCTACCTTGTTACGA- 3ʹ) for forward primer and reverse primer, respectively [ 44 ]. According to the phenotypic resistance profile, each isolate was screened using PCR for the presence of the following antimicrobial resistance genes: bla TEM , bla SHV , bla CTX , bla PER , bla SME , bla KPC , bla Smp , bla Vim , bla Vim-2 , bla NDM , bla OXA , aac (6′)-Ie- aph (2″)-Ia, aph (3′)-IIIa, acc3I, aac3II, aacIII, aac3IV, ant (4′)-Ia, and ant (2′)-Ia.…”

Section: Methodsmentioning

confidence: 99%

Study of Antimicrobial Resistance, Biofilm Formation, and Motility of Pseudomonas aeruginosa Derived from Urine Samples

et al. 2023

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Pseudomonas aeruginosa causes urinary tract infections associated with catheters by forming biofilms on the surface of indwelling catheters. Therefore, controlling the spread of the bacteria is crucial to preventing its transmission in hospitals and the environment. Thus, our objective was to determine the antibiotic susceptibility profiles of twenty-five P. aeruginosa isolates from UTIs at the Medical Center of Trás-os-Montes and Alto Douro (CHTMAD). Biofilm formation and motility are also virulence factors studied in this work. Out of the twenty-five P. aeruginosa isolates, 16% exhibited multidrug resistance, being resistant to at least three classes of antibiotics. However, the isolates showed a high prevalence of susceptibility to amikacin and tobramycin. Resistance to carbapenem antibiotics, essential for treating infections when other antibiotics fail, was low in this study, Notably, 92% of the isolates demonstrated intermediate sensitivity to ciprofloxacin, raising concerns about its efficacy in controlling the disease. Genotypic analysis revealed the presence of various β-lactamase genes, with class B metallo-β-lactamases (MBLs) being the most common. The blaNDM, blaSPM, and blaVIM-VIM2 genes were detected in 16%, 60%, and 12% of the strains, respectively. The presence of these genes highlights the emerging threat of MBL-mediated resistance. Additionally, virulence gene analysis showed varying prevalence rates among the strains. The exoU gene, associated with cytotoxicity, was found in only one isolate, while other genes such as exoS, exoA, exoY, and exoT had a high prevalence. The toxA and lasB genes were present in all isolates, whereas the lasA gene was absent. The presence of various virulence genes suggests the potential of these strains to cause severe infections. This pathogen demonstrated proficiency in producing biofilms, as 92% of the isolates were found to be capable of doing so. Currently, antibiotic resistance is one of the most serious public health problems, as options become inadequate with the continued emergence and spread of multidrug-resistant strains, combined with the high rate of biofilm production and the ease of dissemination. In conclusion, this study provides insights into the antibiotic resistance and virulence profiles of P. aeruginosa strains isolated from human urine infections, highlighting the need for continued surveillance and appropriate therapeutic approaches.

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“…The two primers used were 27F (5′ AGAGTTTGATCCTGGCTCAG-3′) and 1495R (5′ CTACGGCTACCTTGTTACGA-3′). These functions as forward primer and reverse primer, respectively [ 30 ]. Based on their phenotypic resistance profile, each isolate underwent PCR screening for the presence of the following antimicrobial resistance genes: bla TEM , bla SHV , bla CTX , bla PER , bla SME , bla KPC , bla IMP bla Smp , bla Vim , bla Vim-2 , bla NDM , bla OXA , aac (6′)-Ie- aph (2″)-Ia, aph (3′)-IIIa, aac(3)-I, aac(3)-II, aac(3)-III, aac(3)-IV, ant (4′)-Ia and ant (2′)-Ia.…”

Section: Methodsmentioning

confidence: 99%

The Impact of the Virulence of Pseudomonas aeruginosa Isolated from Dogs

Sousa

Garcês

Silva³

et al. 2023

Veterinary Sciences

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Pseudomonas aeruginosa is a pathogenic bacterium that can cause serious infections in both humans and animals, including dogs. Treatment of this bacterium is challenging because some strains have developed multi-drug resistance. This study aimed to evaluate the antimicrobial resistance patterns and biofilm production of clinical isolates of P. aeruginosa obtained from dogs. The study found that resistance to various β-lactam antimicrobials was widespread, with cefovecin and ceftiofur showing resistance in 74% and 59% of the isolates tested, respectively. Among the aminoglycosides, all strains showed susceptibility to amikacin and tobramycin, while gentamicin resistance was observed in 7% of the tested isolates. Furthermore, all isolates carried the oprD gene, which is essential in governing the entry of antibiotics into bacterial cells. The study also investigated the presence of virulence genes and found that all isolates carried exoS, exoA, exoT, exoY, aprA, algD, and plcH genes. This study compared P. aeruginosa resistance patterns worldwide, emphasizing regional understanding and responsible antibiotic use to prevent multi-drug resistance from emerging. In general, the results of this study emphasize the importance of the continued monitoring of antimicrobial resistance in veterinary medicine.

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Molecular characterization of Pseudomonas aeruginosa isolates from Sudanese patients: A cross-sectional study

Cited by 5 publications

References 18 publications

Disinfection of Tank Water Using Biogenic Silver Nanosilica as Anti-Microbial Agents in Jeddah City

Disinfection of Tank Water Using Biogenic Silver Nanosilica as Anti-Microbial Agents in Jeddah City

Study of Antimicrobial Resistance, Biofilm Formation, and Motility of Pseudomonas aeruginosa Derived from Urine Samples

The Impact of the Virulence of Pseudomonas aeruginosa Isolated from Dogs

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