Molecular Characterization of Prospectively Isolated Multipotent Mesenchymal Progenitors Provides New Insight into the Cellular Identity of Mesenchymal Stem Cells in Mouse Bone Marrow

Qian, Hong; Badaloni, Aurora; Chiara, Francesca; Stjernberg, Jenny; Polisetti, Naresh; Nihlberg, Kristian; Consalez, G. Giacomo; Sigvardsson, Mikael

doi:10.1128/mcb.01287-12

Cited by 33 publications

(29 citation statements)

References 56 publications

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“…1). Cancer-associated fibroblasts will be considered as a single entity without regarding potential multiplicity caused by the source for recruitment, such as resident fibroblasts, mesenchymal stem cells [17], bone marrow-derived mesenchymal progenitors [18] or circulating fibrocytes [19].…”

Section: Cancer-associated Fibroblastsmentioning

confidence: 99%

Functional subsets of mesenchymal cell types in the tumor microenvironment

Cortez

Roswall

Pietras

2014

Seminars in Cancer Biology

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In the field of tumor biology, increasing attention is now focused on the complex interactions between various constituent cell types within the tumor microenvironment as being functionally important for the etiology of the disease. The detailed description of tumor-promoting properties of cancer-associated fibroblasts, endothelial cells, pericytes, and immune cells, introduces novel potential drug targets for improved cancer treatments, as well as a rationale for exploring the tumor stroma as a previously unchartered source for prognostic or predictive biomarkers.However, recent work highlights the fact that cellular identity is perhaps too broadly defined and that subdivision of each cell type may reveal functionally distinct subsets of cells. Here, we will review our current understanding of the diversity of different subsets of mesenchymal cells, i.e., cancer-associated fibroblasts and pericytes, residing within the tumor parenchyma.

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Section: Cancer-associated Fibroblastsmentioning

confidence: 99%

Functional subsets of mesenchymal cell types in the tumor microenvironment

Cortez

Roswall

Pietras

2014

Seminars in Cancer Biology

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“…007909. Ebf2 GFP knock-in mice and Ebf2-GFP TG BAC (bacterial artificial chromosome)-transgenic mice were described before (37,38). Ebf2-GFP TG mice were developed by the Mutant Mouse Regional Resource Center.…”

Section: Methodsmentioning

confidence: 99%

Ebf2 is a selective marker of brown and beige adipogenic precursor cells

Wang

Kissig

Rajakumari

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

189

199

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Brown adipocytes and muscle and dorsal dermis descend from precursor cells in the dermomyotome, but the factors that regulate commitment to the brown adipose lineage are unknown. Here, we prospectively isolated and determined the molecular profile of embryonic brown preadipose cells. Brown adipogenic precursor activity in embryos was confined to platelet-derived growth factor α + , myogenic factor 5 Cre -lineage-marked cells. RNA-sequence analysis identified early B-cell factor 2 (Ebf2) as one of the most selectively expressed genes in this cell fraction. Importantly, Ebf2-expressing cells purified from Ebf2 GFP embryos or brown fat tissue did not express myoblast or dermal cell markers and uniformly differentiated into brown adipocytes. Interestingly, Ebf2-expressing cells from white fat tissue in adult animals differentiated into brown-like (or beige) adipocytes. Loss of Ebf2 in brown preadipose cells reduced the expression levels of brown preadipose-signature genes, whereas ectopic Ebf2 expression in myoblasts activated brown preadipose-specific genes. Altogether, these results indicate that Ebf2 specifically marks and regulates the molecular profile of brown preadipose cells.beige adipocyte | brown adipose tissue

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“…27,28 Transplantations BM cells from 7-to 10-week-old Sipa1 mice (lethally irradiated) were used as recipients. At the endpoints (7-9 months) of the primary transplantations, 3 million spleen cells from the primary Sipa1 2/2 recipients (CD45.2), which had developed MDS/MPN, were transplanted into sublethally irradiated Sipa1 1/1 recipients (CD45.2).…”

Section: Cfu-f Assaymentioning

confidence: 99%

Sipa1 deficiency–induced bone marrow niche alterations lead to the initiation of myeloproliferative neoplasm

et al. 2018

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Key Points• Sipa1 loss leads to BM niche alterations prior to the initiation of MPN.• Sipa1-deficient BM niche induces lethal MPN from normal hematopoietic cells.Mutations of signal-induced proliferation-associated gene 1 (SIPA1), a RAP1 GTPaseactivating protein, were reported in patients with juvenile myelomonocytic leukemia, a childhood myelodysplastic/myeloproliferative neoplasm (MDS/MPN). Sipa1 deficiency in mice leads to the development of age-dependent MPN. However, Sipa1 expression in bone marrow (BM) microenvironment and its effect on the pathogenesis of MPN remain unclear.We here report that Sipa1 is expressed in human and mouse BM stromal cells and downregulated in these cells from patients with MPN or MDS/MPN at diagnosis. By using the

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Molecular Characterization of Prospectively Isolated Multipotent Mesenchymal Progenitors Provides New Insight into the Cellular Identity of Mesenchymal Stem Cells in Mouse Bone Marrow

Cited by 33 publications

References 56 publications

Functional subsets of mesenchymal cell types in the tumor microenvironment

Functional subsets of mesenchymal cell types in the tumor microenvironment

Ebf2 is a selective marker of brown and beige adipogenic precursor cells

Sipa1 deficiency–induced bone marrow niche alterations lead to the initiation of myeloproliferative neoplasm

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