Molecular Characterization of Postnatal Development of Testicular Steroidogenesis in Luteinizing Hormone Receptor Knockout Mice

Zhang, Fuping; Pakarainen, Tomi; Zhu, Fei; Poutanen, Matti; Huhtaniemi, Ilpo

doi:10.1210/en.2003-1049

Cited by 120 publications

(114 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The 8 week long testosterone replacement regimen started at the prepubertal age of 21 days to restore both anatomical abnormalities and accessory sex organ function in these hypogonadal LuRKO male mice. [163][164][165] Additionally, in some LuRKO mice testosterone replacement also induced severe inflammation in the coagulating glands, vasa deferentia, and epididymis. 162 Long-term administration of testosterone in Lobund-Wistar and Noble rat strains has been shown to induce invasive prostate adenocarcinomas.…”

Section: Mouse Models Of Prostatitismentioning

confidence: 99%

Experimental rodent models of prostatitis: limitations and potential

Vykhovanets

Resnick

MacLennan

et al. 2007

Prostate Cancer Prostatic Dis

View full text Add to dashboard Cite

Prostatitis is a polyetiological inflammation of the prostate gland in men characterized by pelvic pain, irritative voiding symptoms, and sexual dysfunction. Histologically prostatitis is characterized by poly-and mononuclear cell infiltrates (neutrophils, lymphocytes, macrophages and plasma cells) in the stromal connective tissue around the acini or ducts. Prostatitis is an important worldwide health problem in men. The pathogenesis and diagnostic criteria for the condition are obscure, with the result that the development of management programs for this condition has been hindered. Animal model(s) might be useful in elucidating mechanisms involved in the molecular pathogenesis of chronic nonbacterial prostatitis and chronic pelvic pain syndrome. Given that prostatitis might have a multifactorial etiology, several animal models with unique features may prove helpful. This review examines a number of experimental rodent models of prostatitis and evaluates their advantages and limitations.

show abstract

Section: Mouse Models Of Prostatitismentioning

confidence: 99%

Experimental rodent models of prostatitis: limitations and potential

Vykhovanets

Resnick

MacLennan

et al. 2007

Prostate Cancer Prostatic Dis

View full text Add to dashboard Cite

show abstract

“…In conclusion, poorly developed secondary sex characteristics and infertility are observed in both sexes. Further detail about the phenotype of this model are in our previous reports (Zhang et al, 2001a;Zhang et al, 2003;Zhang et al, 2004;Pakarainen et al, 2005c;Pakarainen et al, 2005b;Pakarainen et al, 2005a).…”

Section: Lh/hcg-r Knockout (Lurko) Micementioning

confidence: 99%

Extragonadal LH/hCG action—Not yet time to rewrite textbooks

Pakarainen¹,

Ahtiainen²,

Zhang³

et al. 2007

Molecular and Cellular Endocrinology

Self Cite

View full text Add to dashboard Cite

Please cite this article as: Pakarainen, T., Ahtiainen, P., Zhang, F.-P., Rulli, S., Poutanen, M., Huhtaniemi, I., Extragonadal LH/hCG action -not yet time to rewrite textbooks, Molecular and Cellular Endocrinology (2007), doi:10.1016/j.mce.2006 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.A c c e p t e d M a n u s c r i p t A c c e p t e d M a n u s c r i p t AbstractGonadotropins are indispensable in both sexes in the regulation of gonadal sex steroid production and gametogenesis. In addition to their well established classical actions, numerous recent publications have indicated the presence and function of luteinizing hormone/chorionic gonadotropin receptors (LH/hCG-R) in a variety of extragonadal tissues. However, the physiological significance of such effects has remained unclear. We have generated two genetically modified mouse models, one with excessive production of hCG and the other with targeted disruption of LH/hCG-R gene, and used them to address the functions of LH and hCG.Numerous gonadal and extragonadal phenotypes were found in the models with the two extremes of LH/hCG action. However, when the extragonadal effects were scrutinized in greater detail, they all appeared to arise through modification of gonadal function, either through enhanced or inhibited response to LH/hCG stimulation. Hence, further evidence is needed before the extragonadal LH/hCG-R expression can be considered functionally significant.

show abstract

“…This work showed that Leydig cell regeneration after EDS is dependent on LH (Molenaar et al ., 1986; Sriraman et al ., 2003) although some early proliferation and differentiation appears to occur independent of LH (Teerds et al ., 1989). Subsequent studies have shown that similar control mechanisms regulate normal adult Leydig cell development at puberty (Clark et al ., 2000; Gnessi et al ., 2000; Baker & O'Shaughnessy, 2001; Zhang et al ., 2001, 2004; Baker et al ., 2003). Other factors such as DHH, PDGF and anti‐Müllerian hormone (AMH) have been implicated in the development of Leydig cells both during development and re‐generation (Salva et al ., 2004; Josso et al ., 2006; O'Shaughnessy et al ., 2008b) and further studies on EDS regeneration have also suggested that nerve growth factor (NGF) and Sertoli cells are involved in Leydig cell proliferation, differentiation and survival (Yan et al ., 2000; Zhang et al ., 2013).…”

Section: Leydig Cellsmentioning

confidence: 99%

Cell‐specific ablation in the testis: what have we learned?

2015

View full text Add to dashboard Cite

SummaryTesticular development and function is the culmination of a complex process of autocrine, paracrine and endocrine interactions between multiple cell types. Dissecting this has classically involved the use of systemic treatments to perturb endocrine function, or more recently, transgenic models to knockout individual genes. However, targeting genes one at a time does not capture the more wide‐ranging role of each cell type in its entirety. An often overlooked, but extremely powerful approach to elucidate cellular function is the use of cell ablation strategies, specifically removing one cellular population and examining the resultant impacts on development and function. Cell ablation studies reveal a more holistic overview of cell–cell interactions. This not only identifies important roles for the ablated cell type, which warrant further downstream study, but also, and importantly, reveals functions within the tissue that occur completely independently of the ablated cell type. To date, cell ablation studies in the testis have specifically removed germ cells, Leydig cells, macrophages and recently Sertoli cells. These studies have provided great leaps in understanding not possible via other approaches; as such, cell ablation represents an essential component in the researchers’ tool‐kit, and should be viewed as a complement to the more mainstream approaches to advancing our understanding of testis biology. In this review, we summarise the cell ablation models used in the testis, and discuss what each of these have taught us about testis development and function.

show abstract

Molecular Characterization of Postnatal Development of Testicular Steroidogenesis in Luteinizing Hormone Receptor Knockout Mice

Cited by 120 publications

References 44 publications

Experimental rodent models of prostatitis: limitations and potential

Experimental rodent models of prostatitis: limitations and potential

Extragonadal LH/hCG action—Not yet time to rewrite textbooks

Cell‐specific ablation in the testis: what have we learned?

Contact Info

Product

Resources

About