Molecular Characterization of Porcine Picobirnaviruses and Development of a Specific Reverse Transcription-PCR Assay

Carruyo, Gabriela; Mateu, Guaniri; Martı́nez, Laura C.; Pujol, Flor H.; Nates, Silvia; Liprandi, Ferdinando; Ludert, Juan E.

doi:10.1128/jcm.00655-08

Cited by 37 publications

(77 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of other studies also suggest that porcine PBV strains that are genetically diverse, are related to human strains, and cause frequent and persistent infections among young pigs with or without diarrhea or any sign of illness [11,56]. So, stringent surveillance is required to monitor the genotype diversity and to determine the possible selective pressures under which PBV variants adapt to different hosts and environmental conditions.…”

Section: Discovery Of Picobirnavirusmentioning

confidence: 99%

See 1 more Smart Citation

Animal Picobirnavirus

2014

View full text Add to dashboard Cite

Picobirnavirus (PBV) is a small, non-enveloped, bisegmented double-stranded RNA (dsRNA) virus of vertebrate hosts. The name 'Picobirnavirus' derives from the prefix 'pico' (latin for 'small') in reference to the small virion size, plus the prefix 'bi' (latin for 'two') and the word 'RNA' to indicate the nature of the viral genome. The serendipitous discovery of PBV dates back to 1988 from Brazil, when human fecal samples collected during the acute gastroenteritis outbreaks were subjected for routine rotavirus surveillance by polyacrylamide gel electrophoresis (PAGE) and silver straining (S/S). The PAGE gels after silver staining showed a typical 'two RNA band' pattern, and it was identified as Picobirnavirus. Likewise, the feces of wild black-footed pigmy rice rats (Oryzomys nigripes) subjected for PAGE assay by the same research group in Brazil reported the presence of PBV (Pereira et al., J Gen Virol 69:2749-2754. PBVs have been detected in faeces of humans and wide range of animal species with or without diarrhoea, worldwide. The probable role of PBV as either a 'primary diarrhoeal agent' in 'immunocompetent children'; or a 'potential pathogen' in 'immunocompromised individuals' or an 'innocuous virus' in the intestine remains elusive and needs to be investigated despite the numerous reports of the presence of PBV in fecal samples of various species of domestic mammals, wild animals, birds and snakes; our current knowledge of their biology, etiology, pathogenicity or their transmission characteristics remains subtle. This review aims to analyse the veterinary and zoonotic aspects of animal Picobirnavirus infections since its discovery.

show abstract

Section: Discovery Of Picobirnavirusmentioning

confidence: 99%

“…Genogroup I PBVs those were detected and sequenced from pigs in Hungary [4], Venezuela and Argentina [11,41], showed close relationship to human genogroup I PBVs. The PBV strains detected among children in Kolkata, India were reported to be closely related to porcine PBV strains [28,29].…”

Section: Discovery Of Picobirnavirusmentioning

confidence: 99%

Animal Picobirnavirus

2014

View full text Add to dashboard Cite

show abstract

“…Genogroup I PBVs detected and sequenced from pigs in Hungary [2] and Venezuela and Argentina [5,22], were observed to be closely related to human genogroup I PBVs. These results strongly suggest that PBV strains may circulate in the shared environment where humans and pigs live.…”

mentioning

confidence: 96%

“…However, the PAGE assay detects the PBVs readily as it is not dependent on their genomic sequences. PBVs are known to cause chronic diarrhoea with prolonged shedding of the virus in humans [24] and various animals [30] besides frequent infections among piglets [5,29]. Moreover, the presence of genogroup I PBVs in humans and different animals, rodents and reptiles, suggests that any specific genogroup is not restricted to specific host [2,3,5,12,16,19,31].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Detection and Molecular Characterization of Porcine Picobirnavirus in Feces of Domestic Pigs from Kolkata, India

et al. 2012

View full text Add to dashboard Cite

Picobirnaviruses (PBVs) are small, non-enveloped, 35-41 nm virion with bisegmented double-stranded RNA genome. PBVs are widespread and were detected in feces of humans and a wide variety of animals. Domestic pig, one of the ubiquitous farm animal reported incessant association with a variety of viral zoonoses. The objective of our study is to find out the incidence of PBV infection in healthy domestic pigs. The study was conducted by collecting feces of healthy/asymptomatic pigs from a piggery located in an urban slum at Kolkata, India to detect PBV infections. All the 11 fecal samples were tested by polyacrylamide gel electrophoresis and reverse transcriptionpolymerase chain reaction assay. In this study, we report the first incidence of detection and molecular characterization of porcine PBV (BG-Por-2/2010 and BG-Por-7/ 2010) in feces of domestic pigs from India using the human PBV genogroup I specific primer pair: PicoB25(?) and PicoB43(-). Sequence comparison and phylogenetic analysis of partial RNA-dependent RNA polymerase gene of genome segment 2 revealed genetic relatedness to hitherto reported porcine, murine and human genogroup I PBVs from different geographical regions. This warrants a stringent global surveillance to study the potential zoonotic and emerging PBV infections.

show abstract

Picobirnavirus infections: viral persistence and zoonotic potential

Ganesh

Bányai

Martella

et al. 2012

Reviews in Medical Virology

View full text Add to dashboard Cite

Picobirnaviruses (PBVs) are small, non-enveloped, bisegmented double-stranded RNA genomic viruses of vertebrate hosts. Since their discovery in the late 1980s in clinical specimens from outbreaks of acute gastroenteritis in children, significant efforts have been made to investigate the role of PBV in diarrheic diseases. PBV has been detected in sporadic episodes of diarrhea as sole pathogen or coinfection as well as in outbreaks of acute gastroenteritis and in immunocompromised patients with diarrhea. However, PBV is frequently detected in non-diarrheic healthy hosts, and prolonged shedding has been observed in some individuals. Of interest, similar patterns of PBV infection have also been observed in pigs and other animal hosts. The increasing amount of PBV sequence data gathered from molecular epidemiological studies has evidenced a great sequence diversity of PBVs in various hosts and environmental samples. Importantly, evidence has been found for genetic relatedness between human and animal PBV strains, suggesting extant crossing points in the ecology and evolution of heterologous PBV strains. At present, no cell culture and animal model exists for PBVs. Well-structured epidemiological studies are still the only alternative to demonstrate the potential etiological role of PBVs in acute gastroenteritis or other diseases. This review aims to analyze the public health aspects of PBV infection, especially its possible association with zoonosis.

show abstract

Molecular Characterization of Porcine Picobirnaviruses and Development of a Specific Reverse Transcription-PCR Assay

Cited by 37 publications

References 28 publications

Animal Picobirnavirus

Animal Picobirnavirus

Detection and Molecular Characterization of Porcine Picobirnavirus in Feces of Domestic Pigs from Kolkata, India

Picobirnavirus infections: viral persistence and zoonotic potential

Contact Info

Product

Resources

About