Molecular characterization of polish blueberry red ringspot virus isolate

Kalinowska, E.; Paduch-Cichal, E.; Chodorska, M.

doi:10.1007/s11262-011-0679-4

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…ORF V (2,065–4107 nt) encodes the RNA-dependent DNA polymerase (RT) that contains 680 amino acids and is estimated to be 78 kDa. The RT contains some of the most conserved amino acid motifs indicating a common evolutionary origin of Caulimoviridae family [1, 20] As expected, the RT from WCSV-1 contains the conserved amino acid sequence (YIDDVIIF) for the putative RT domain found in other caulimoviruses at amino acid positions 351–358 [4]. The consensus sequences for RNase H were also identified (Fig.…”

Section: Resultsmentioning

confidence: 79%

Identification and characterization of water chestnut Soymovirus-1 (WCSV-1), a novel Soymovirus in water chestnuts (Eleocharis dulcis)

et al. 2019

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Background A disease of unknown etiology in water chestnut plants ( Eleocharis dulcis ) was reported in China between 2012 and 2014. High throughput sequencing of small RNA (sRNA) combined with bioinformatics, and molecular identification based on PCR detection with virus-specific primers and DNA sequencing is a desirable approach to identify an unknown infectious agent. In this study, we employed this approach to identify viral sequences in water chestnut plants and to explore the molecular interaction of the identified viral pathogen and its natural plant host. Results Based on high throughput sequencing of virus-derived small RNAs (vsRNA), we identified the sequence a new-to-science double-strand DNA virus isolated from water chestnut cv. ‘Tuanfeng’ samples, a widely grown cultivar in Hubei province, China, and analyzed its genomic organization. The complete genomic sequence is 7535 base-pairs in length, and shares 42–52% nucleotide sequence identity with viruses in the Caulimoviridae family. The virus contains nine predicated open reading frames (ORFs) encoding nine hypothetical proteins, with conserved domains characteristic of caulimoviruses. Phylogenetic analyses at the nucleotide and amino acid levels indicated that the virus belongs to the genus Soymovirus . The virus is tentatively named Water chestnut soymovirus-1 (WCSV-1). Phylogenetic analysis of the putative viral polymerase protein suggested that WCSV-1 is distinct to other well established species in the Soymovirus genus. This conclusion was supported by phylogenetic analyses of the amino acid sequences encoded by ORFs I, IV, VI, or VII. The sRNA bioinformatics showed that the majority of the vsRNAs are 22-nt in length with a preference for U at the 5′-terminal nucleotide. The vsRNAs are unevenly distributed over both strands of the entire WCSV-1 circular genome, and are clustered into small defined regions. In addition, we detected WCSV-1 in asymptomatic and symptomatic water chestnut samples collected from different regions of China by using PCR. RNA-seq assays further confirmed the presence of WCSV-1-derived viral RNA in infected plants. Conclusions This is the first discovery of a dsDNA virus in the genus Soymovirus infecting water chestnuts. Data presented also add new information towards a better understanding of the co-evolutionary mechanisms between the virus and its natural plant host. Electronic supplementary material The online version of this article (10.1186/s12870-019-1761-7) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 79%

Identification and characterization of water chestnut Soymovirus-1 (WCSV-1), a novel Soymovirus in water chestnuts (Eleocharis dulcis)

et al. 2019

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“…Red ringspot disease originally was described in New Jersey with associated symptoms observed on highbush blueberry in the 1950s [43] and since then has rapidly expanded to other states in the US, as well as other countries including Czech Republic, Japan, Korea, Poland, Serbia and Slovenia [10,34,[43][44][45][46][47][48][49]. Symptoms are usually seen in late summer and early fall on older leaves as red blotches resulted from the coalescence of round red spots (Figure 3A).…”

Section: Blueberry Red Ringspot Virus (Brrv)mentioning

confidence: 99%

Known and New Emerging Viruses Infecting Blueberry

Olmstead

Jones

Varsani

et al. 2021

Plants

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Blueberry (Vaccinium spp.) plants are exposed to existing and emerging viruses as a result of expanding acreage of blueberry plantations across the world, primarily in North America. Since blueberry is cultivated in areas where there are wild Vaccinium spp., there is increasing risk of virus movement between wild and cultivated blueberries. This is theoretically possible because viruses can spread from commercial cultivars to native species and vice versa causing the spread of existing and new viruses. The occurrence of these viruses in blueberry can be devastating to the industry considering the cost for cultivation and production of this perennial crop. However, the advent of high-throughput sequencing and bioinformatic sequence analysis have allowed for rapid identification of known and novel viruses in any crop including blueberry, thus facilitating proper intervention in response to serious viral diseases. In this paper, we aim to focus on the current status of known and novel viruses emerging in blueberry worldwide, which may impact the blueberry industry.

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“…To date, the virus has been detected outside of North America on imported plants in Japan (Isogai et al 2009), and in the three European countries: Slovenia, Czech Republic, and Poland (Mavrič Pleško et al 2009;Přibylová et al 2010;Kalinowska et al 2012). The infection in Slovenia was found in one plant of cv.…”

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confidence: 99%

Blueberry red ringspot virus eliminated from highbush blueberry by shoot tip culture

et al. 2014

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AbstractŠpak J., Pavingerová D., Přibylová J., Špaková V., Paprštein F., Sedlák J. (2013): Blueberry red ringspot virus eliminated from highbush blueberry by shoot tip culture. Plant Protect. Sci., 50: 174-178.In order to find an effective elimination method of Blueberry red ringspot virus (BRRV), shoot tips of 2-3 mm size originating from buds of highbush blueberry cv. Darrow naturally infected by BRRV and collected in winter months were regenerated and multiplied on woody-plant medium (WPM) with 1.5 mg/l zeatin and 20 g/l sucrose. Developed shoots tested negative by polymerase chain reaction (PCR) for BRRV were transferred onto a rooting medium consisting of WPM supplemented with 1 mg/l indole-3-butyric acid. Rooting plants were transferred into soil and tested twice by PCR 6 months apart. Ninety per cent eradication of BRRV was achieved while isolating shoot tips, multiplying them in one vegetative generation, and regenerating whole 50-60 cm high plants within 30 months. All plants developed rich fruits after their overwintering in a cold greenhouse and were tested BRRV negative in summer 2013.

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Molecular characterization of polish blueberry red ringspot virus isolate

Cited by 3 publications

References 7 publications

Identification and characterization of water chestnut Soymovirus-1 (WCSV-1), a novel Soymovirus in water chestnuts (Eleocharis dulcis)

Identification and characterization of water chestnut Soymovirus-1 (WCSV-1), a novel Soymovirus in water chestnuts (Eleocharis dulcis)

Known and New Emerging Viruses Infecting Blueberry

Blueberry red ringspot virus eliminated from highbush blueberry by shoot tip culture

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