Molecular characterization of PgUFGT gene and R2R3-PgMYB transcription factor involved in flavonoid biosynthesis in four tissues of wild pomegranate (Punica granatum L.)

Kaur, Ramanjeet; Kapoor, Nisha; Aslam, Lubna; Mahajan, Ritu

doi:10.1007/s12041-019-1141-y

Cited by 12 publications

(4 citation statements)

References 62 publications

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“…Of the flavonoids, cyanidin-3- O -rutinoside, peonidin-3,5- O -diglucoside, cyanidin-3- O -glucoside, and cyanidin were the most abundant anthocyanin substances. Two types of genes are involved in anthocyanin biosynthesis: structural genes that encode enzymes in metabolic pathways that catalyze anthocyanin biosynthesis and regulatory genes that control the transcription of these structural genes. ,,,− , …”

Section: Discussionsupporting

confidence: 60%

“…Two types of genes are involved in anthocyanin biosynthesis: structural genes that encode enzymes in metabolic pathways that catalyze anthocyanin biosynthesis and regulatory genes that control the transcription of these structural genes. 1,3,15,[46][47][48]56 Through KEGG enrichment analysis and gene functional annotation, 11 DEGs were identified as anthocyanin biosynthesis candidate genes, including the structural genes ZjANS, ZjUGT79B1, and ZjCCoAOMT. The transcription factor genes ZjMYB113 and ZjMYB5 had expression patterns similar to those of ANS and UFGT, and their expression trends were closely related to the contents of cyanidin, cyanidin-3-O-rutinoside, and peonidin-3,5-O-diglucoside.…”

Section: ■ Discussionmentioning

confidence: 96%

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Metabolomic and Transcriptomic Analyses of Anthocyanin Biosynthesis Mechanisms in the Color Mutant Ziziphus jujuba cv. Tailihong

Shi

Zhu

et al. 2020

J. Agric. Food Chem.

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The purplish-red color of “Tailihong” jujube fruit skins is caused primarily by anthocyanin accumulation, but the mechanisms that underlie anthocyanin biosynthesis in jujube fruit have rarely been studied. We performed metabolomic and transcriptomic analyses of jujube fruit skins at different developmental stages and identified a total of 158 flavonoids, among which cyanidin-3-O-rutinoside and peonidin-3,5-O-diglucoside were the primary anthocyanins. During fruit development and maturation, the anthocyanin content was strongly correlated with the expression of ZjANS and ZjUGT79B1, suggesting that these are key genes in the anthocyanin biosynthesis process. Transcriptomic analysis indicated that the transcription factors ZjMYB5, ZjTT8, and ZjWDR3 regulated anthocyanin biosynthesis in jujube fruit skins. Subcellular localization experiments confirmed that ZjANS and ZjUGT79B1 were localized to the nucleus and the endoplasmic reticulum. ZjMYB5 and ZjTT8 were found only in the nucleus, whereas strong fluorescence signals from ZjWDR3 were observed in the nucleus and cytoplasm. Prokaryotic expression and in vitro enzyme activity assays showed that the recombinant ZjANS protein catalyzed the formation of cyanidin from (+)-catechin. Secondary glycosylation by ZjUFGT79B1 modified cyanidin-3-O-glucoside to produce cyanidin-3-O-rutinoside, and ZjCCoAOMT readily catalyzed the production of the methylated anthocyanin peonidin-3,5-O-diglucoside from cyanidin 3,5-O-glucoside. Dual-Luciferase and GUS activity assays showed that the ZjANS and ZjUGT79B1 promoters were activated by ZjMYB5, ZjTT8, and ZjWDR3. All data indicated that these three transcription factors played important roles in anthocyanin biosynthesis in the color mutant Ziziphus jujuba cv. Tailihong, contributing to anthocyanin accumulation by enhancing the expression of ZjANS and ZjUGT79B1.

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Section: Discussionsupporting

confidence: 60%

Section: ■ Discussionmentioning

confidence: 96%

Metabolomic and Transcriptomic Analyses of Anthocyanin Biosynthesis Mechanisms in the Color Mutant Ziziphus jujuba cv. Tailihong

Shi

Zhu

et al. 2020

J. Agric. Food Chem.

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“…The primers used for qRT-PCR are listed in (Table 1). And, the PgActin gene was used as the reference gene [43,44].…”

Section: Extraction Of Rna and Qrt-pcr Validationsmentioning

confidence: 99%

Effects of 5-Aminolevulinic Acid (5-ALA) on Physicochemical Characteristics and Growth of Pomegranate (Punica granatum L.)

Liu

et al. 2023

Horticulturae

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The pomegranate is one of the most essential crop fruits in the world. 5-Aminolevulinic acid (ALA) regulates the growth and development of 5-year-old pomegranate. However, for plenty of pome crops, conventional information on the physio-chemical characteristics underlying 5-ALA is limited. Therefore, in this study, we applied four different concentrations of 5-ALA treatments (0 mg L−1, 10 mg L−1, 20 mg L−1, 50 mg L−1, and 100 mg L−1), where 0 mg L−1 was considered as a control group, to evaluate the effects of 5-ALA on the physiochemical characteristics of pomegranates. Our results showed that maximal photochemical efficiency (FV/FM), photosystem (ΦPSII), and photochemical quenching (qP) increased at concentrations of 50 mg L−1 and 100 mg L−1 compared with controls. Anthocyanin contents were elevated by 2.27% and 1.33% at the 5-ALA concentrations of 50 mg L−1 and 100 mg L−1. Furthermore, qRT-PCRs of the DEGs, such as punica granatum chalcone synthase (PgCHS), chalcone isomerase (PgCHI), flavanone 3-hydroxylase (PgF3H), dihydroflavonol 4-reductase (PgDFR), anthocyanidin synthase (PgANS), and ultrafine-grained (PgUFG), showed up-regulations, except for chalcone isomerase (PgCHI), after 5-ALA treatments. The fresh weight of the fruit and the weight of the grains were elevated under 50 mg L−1 and 100 mg L−1 concentrations, and both the fresh weight of the fruit and the grain weight were higher than controls. Total sugar (TS) increased by 8.49% and 24.99%, total soluble solids (TSS) increased by 2.02% and 6.07%, soluble proteins increased by 1.16% and 1.15%, and the pH level of juice increased by 0.12% and 0.19%, respectively. In addition, the contents of ascorbic acid, total phenols, and total flavonoids increased by 24.18%, 2.53%, and 1.29%, respectively, compared with controls. Taken together, the antioxidant activities of SOD and POD also increased by 13.33% and 11.95% at concentrations of 100 mg L−1. Our results show that concentrations of 5-ALA treatments at 50 mg L−1 and 100 mg L−1 will be beneficial for crop cultivation.

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“…In Malus domestica (Md), MdMYB1 can directly bind to the promoter of MdGSTF6 to promote the transfer of anthocyanin to a vacuole [6]; depending on the co-expression of MdbHLH3 and MdbHLH33, MdMYB10 induces efficient anthocyanin biosynthesis in transient assays [7]. In Oryza sativa (rice), the MBW complex formed by R2R3-MYB can activate the expression of all the structural genes involved in anthocyanin synthesis in leaves [8], while in pomegranate, R2R3-PgMYB controls the synthesis of coloration through UFGT [9]. A similar effect of R2R3-MYB has been found in plants such as pears (Pyrus ussuriensis) and peaches (Prunus persica) [10,11].…”

Section: Introductionmentioning

confidence: 99%

A Fruit-Expressed MYB Transcription Factor Regulates Anthocyanin Biosynthesis in Atropa belladonna

Liu,

Zhao,

Yuan

et al. 2024

IJMS

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Anthocyanins are water-soluble flavonoid pigments that play a crucial role in plant growth and metabolism. They serve as attractants for animals by providing plants with red, blue, and purple pigments, facilitating pollination and seed dispersal. The fruits of solanaceous plants, tomato (Solanum lycopersicum) and eggplant (Solanum melongena), primarily accumulate anthocyanins in the fruit peels, while the ripe fruits of Atropa belladonna (Ab) have a dark purple flesh due to anthocyanin accumulation. In this study, an R2R3-MYB transcription factor (TF), AbMYB1, was identified through association analysis of gene expression and anthocyanin accumulation in different tissues of A. belladonna. Its role in regulating anthocyanin biosynthesis was investigated through gene overexpression and RNA interference (RNAi). Overexpression of AbMYB1 significantly enhanced the expression of anthocyanin biosynthesis genes, such as AbF3H, AbF3′5′H, AbDFR, AbANS, and Ab3GT, leading to increased anthocyanin production. Conversely, RNAi-mediated suppression of AbMYB1 resulted in decreased expression of most anthocyanin biosynthesis genes, as well as reduced anthocyanin contents in A. belladonna. Overall, AbMYB1 was identified as a fruit-expressed R2R3-MYB TF that positively regulated anthocyanin biosynthesis in A. belladonna. This study provides valuable insights into the regulation of anthocyanin biosynthesis in Solanaceae plants, laying the foundation for understanding anthocyanin accumulation especially in the whole fruits of solanaceous plants.

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Molecular characterization of PgUFGT gene and R2R3-PgMYB transcription factor involved in flavonoid biosynthesis in four tissues of wild pomegranate (Punica granatum L.)

Cited by 12 publications

References 62 publications

Metabolomic and Transcriptomic Analyses of Anthocyanin Biosynthesis Mechanisms in the Color Mutant Ziziphus jujuba cv. Tailihong

Metabolomic and Transcriptomic Analyses of Anthocyanin Biosynthesis Mechanisms in the Color Mutant Ziziphus jujuba cv. Tailihong

Effects of 5-Aminolevulinic Acid (5-ALA) on Physicochemical Characteristics and Growth of Pomegranate (Punica granatum L.)

A Fruit-Expressed MYB Transcription Factor Regulates Anthocyanin Biosynthesis in Atropa belladonna

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