Molecular Characterization of Peruvian  Fowl Adenovirus (FAdV) Isolates

Rodríguez, Jorge; Koga, Ysabel; Alvarado, Arnaldo; Tinoco, Robert

doi:10.4236/aim.2014.410065

Cited by 6 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Avian adenovirus has been reported in many countries and is a virus associated with diseases such as inclusion body hepatitis (IBH), hydropericardium syndrome (HS), and respiratory and enteric conditions [2,4,6,14,20,24]. FAdV-I has also been described in South American countries like Ecuador, Chile and Peru [16,26,31], including Brazil [22]. In this study, FAdV-I was detected in its target tissues where replication occurs [7,29], based on PCR amplification of the 897 bp fragment of the hexon gene [19].…”

Section: Sequencing Analysis and Phylogenetic Treementioning

confidence: 99%

Molecular characterization of fowl adenovirus group I in commercial broiler chickens in Brazil

et al. 2018

View full text Add to dashboard Cite

Avian adenovirus has been reported in many countries and is an infectious agent related with inclusion body hepatitis, hepatitis-hydropericardium syndrome (HHS), and respiratory and enteric conditions in chickens worldwide. The objective of this study was to detect and establish the molecular sequences of the hexon gene from the avian adenovirus strains of group I (FAdV-I) isolated from birds with hepatitis-hydropericardium syndrome (HHS), malabsorption syndrome and runting-stunting syndrome, to characterize the serotype of virus affecting commercial flocks in Brazil. Molecular characterization was performed by polymerase chain reaction (PCR), using specific primers to amplify the Loop 1 (L1) variable region of the gene in the FAdV-I genome and subsequent sequencing of the PCR product for each positive sample. The results have revealed the presence of the FAdV-8a, FAdV-8b, and FAdV-11 serotypes circulating in Brazilian chicken flocks. Phylogenetic analysis grouped these sequences into three (3) distinct groups, 14 samples were aligned with the FAdV-11 group, three (3) samples in the FAdV-8b group and one (1) sample in the FAdV-8a group. The serotypes FAdV-8a, FAdV-8b, and FAdV-11 are circulating in Brazilian chicken flocks. Therefore, these results are very important for improvement biosecurity measurements and vaccine production.

show abstract

Section: Sequencing Analysis and Phylogenetic Treementioning

confidence: 99%

Molecular characterization of fowl adenovirus group I in commercial broiler chickens in Brazil

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Serological detections have been reported in Cuba, Guyana and Trinidad & Tobago and molecular detection of the virus has been reported in the Dominica Republic (Brown Jordan, Gongora, Hartley, & Oura, 2018;Brown Jordan, Sookhoo, et al, 2018;Schachner, Marek, Grafl, & Hess, 2016). There have however been a number of reports of FAdV being present and causing disease in poultry from the nearby territories of Latin America, including the presence of FAdV-4 (Hafez, 2011;Liu et al, 2016;Mazaheri et al, 1998;Pereira et al, 2014;Rodríguez, Koga, Alvarado, & Tinoco, 2014;Toro et al, 1999). A recent publication on the molecular characterization of disease causing FAdV strains from Brazil revealed the presence of FAdV-8a, FAdV-8b and FAdV-11 (De la Torre, Nunez, Parra, Astolfi-Ferreira, & Ferreira, 2018).…”

Section: Introductionmentioning

confidence: 99%

Identification of four serotypes of fowl adenovirus in clinically affected commercial poultry co‐infected with chicken infectious anaemia virus in Trinidad and Tobago

Jordan

Blake

Bisnath³

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

Summary Fowl adenovirus (FAdV), which causes the high‐impact diseases such as inclusion body hepatitis and hepatitis‐hydropericardium syndrome, is of major concern to the poultry industry internationally. This study was carried out in direct response to mortality rates of up to 75% in commercial broiler flocks in Trinidad, West Indies. Symptoms in 3‐ to 8‐week‐old broilers and 13‐ to 18‐week‐old pullets pointed to infection with an immunosuppressive viral pathogen. The objectives of the study were to determine whether the infectious agent FAdV, along with other viral pathogens, was responsible for the clinical disease, and to obtain information on the serotypes of FAdV that were infecting the birds. Tissue samples from clinically affected birds from eight different farms were tested for chicken infectious anaemia virus (CIAV) and infectious bursal disease virus (IBDV) by real‐time reverse transcription polymerase chain reaction (PCR) and for FAdV by conventional PCR. The birds tested positive for FAdV and CIAV, but negative for IBDV. The gene corresponding to the L1 loop of the hexon protein for FAdV was amplified and sequenced. Phylogenetic analysis of seven FAdV strains inferred that four serotypes were likely to be circulating in the chickens. Well supported genetic relatedness was observed for serotype 8a (97.8%), 8b (97.8%), 9 (95.8%) and 11 (98.8%–99.5%). This is the first published report from Trinidad and Tobago on the presence and circulation of pathogenic FAdV strains, in combination with CIAV, in poultry. The data demonstrate a possible need for the introduction of serotype‐specific vaccines against FAdV, as well as vaccines against CIAV, in broilers in the region and emphasize the importance of maintaining high levels of biosecurity on farms to prevent the spread of these potentially devastating viruses between farms.

show abstract

“…In a report from Peru of C-4 isolates from outbreaks of adenoviral hepatitis consistent with IBH in broilers, hydropericardium was absent. 11 The 789-bp sequences analyzed in Peru are considerably different from the C-4 sequenced from our cases, and further comparisons cannot be made. Nevertheless, the serotype identified in the backyard chickens described here is identical to the strains identified in HHS outbreaks in China, and we cannot account for this discrepancy.…”

Section: Brief Reportmentioning

confidence: 64%

Emergence of fowl aviadenovirus C-4 in a backyard chicken flock in California

et al. 2021

View full text Add to dashboard Cite

Fowl aviadenovirus (FAdV) species D and E are associated with inclusion body hepatitis (IBH); species C, serotype 4 (hereafter, FAdV4) is associated with hepatitis–hydropericardium syndrome (HHS) in young chickens. Outbreaks of HHS have led to significant losses in the poultry industry in several countries, predominantly in China. In April 2020, FAdV4 was detected in a remote backyard flock in California. In a mixed flock of chickens of various breeds and ages (6 mo to 2 y old), 7 of 30 were found dead within a week without premonitory signs. One additional bird died after the flock was relocated to fresh pasture, bringing the total mortality to 8 of 30 (27%). Postmortem examination of 3 birds revealed good body condition scores and active laying. One chicken had subtle hemorrhages throughout the liver, and the other 2 had diffusely dark mahogany livers. On histopathology, 2 chickens had hepatic necrosis with hepatocytes containing large, mostly basophilic, intranuclear inclusion bodies, identified by electron microscopy as 82.2-nm diameter adenoviral particles. Virus isolation and genomic sequencing performed on a liver sample revealed strains with 99.9% homology to FAdV4 isolates reported from China. To our knowledge, FAdV4 has not been reported in the United States to date. Furthermore, the chickens affected here were all adults and exhibited a variation of serotype 4 disease in which IBH was present but not hydropericardium.

show abstract

Molecular Characterization of Peruvian Fowl Adenovirus (FAdV) Isolates

Cited by 6 publications

References 24 publications

Molecular characterization of fowl adenovirus group I in commercial broiler chickens in Brazil

Molecular characterization of fowl adenovirus group I in commercial broiler chickens in Brazil

Identification of four serotypes of fowl adenovirus in clinically affected commercial poultry co‐infected with chicken infectious anaemia virus in Trinidad and Tobago

Emergence of fowl aviadenovirus C-4 in a backyard chicken flock in California

Contact Info

Product

Resources

About