Molecular Characterization of Penicillium expansum Isolated from Grapes and its Management by Leaf Extract of Chenopodium murale

Khan, Iqra Haider; Javaid, Arshad; Naqvi, Syeda Fakehha

doi:10.33687/phytopath.010.01.3506

Cited by 6 publications

(3 citation statements)

References 28 publications

(26 reference statements)

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“…Grape pomace fluid extract had considerable potency against the Candida species C. albicans , C. krusei and C. parapsilosis 26 . Other study by Khan et al 27 revealed effective antifungal activity of grape leaves extracts against Penicillium expansum. In current study the ethanolic grape peduncles EGP extract was analyzed by agar mix method and resulted in potent antifungal activity against three fungal strains E. floccosum , S. cerevisiae and T. tonsurans.…”

Section: Discussionmentioning

confidence: 90%

High throughput biochemical profiling, and functional potential analysis for valorization of grape peduncles

Akbar

Gul

Hussain

et al. 2023

Sci Rep

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Vitis vinifera L., commonly known as grape is a major fruit crop in the world. Grapes seem to confer health benefits due to their chemical components, biological and antioxidant activities. The present study is conducted to evaluate the biochemical constituents, antioxidant, and antimicrobial potential of ethanolic grape peduncles (EGP) extract. The result of phytochemical analysis revealed the presence of various phytochemicals such as flavonoid, tannin, carbohydrates, alkaloids, cardiac glycoside, phenol, steroid, terpenoids, quinones and anthraquinones. Furthermore, total phenolic content (TPC) and total flavonoid contents (TFC) were 7.35 ± 0.25 mg GAE/g (Gallic Acid Equivalent per gram) and 29.67 ± 0.13 mg QE/g (Quercetin Equivalent per gram) respectively. DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging assay revealed IC50 = 159.3 μg/mL. The antibacterial and antifungal study disclosed that the extract was highly potent against Salmonella typhi with maximum zone of inhibition of 27.2 ± 1.60 mm and Epidermophyton floccosum with 74 ± 1.81% inhibition. The extract was analyzed for its cytotoxicity and antileishmanial activity and showed no activity against HeLa cell line and promastigotes of Leishmania major. Elements Fe, Mn, Ni, Pb and Cd were determined by atomic absorption spectroscopy and approximately 50 compounds were identified by Gas Chromatography-Mass Spectrometry (GC–MS). Current work suggest that grape peduncles can be a promising source of bioactive medicinal component.

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Section: Discussionmentioning

confidence: 90%

High throughput biochemical profiling, and functional potential analysis for valorization of grape peduncles

Akbar

Gul

Hussain

et al. 2023

Sci Rep

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“…After morphological identification for conforming identification of pathogen molecular sequencing was outsourced from Eurofins Genomics, Bengaluru by using ITS1/ITS4 markers [42,43], yielded 706 bp amplicons (Plate 3) (Fig. 3) identified as binucleate Rhizoctonia AG -E with a telemorphic stage Ceratobasidium sp.…”

Section: Molecular Confirmation Of the Pathogenmentioning

confidence: 99%

Survey, Pathogenicity and Molecular Characterization of Root Pathogens Associated with Root Rot of Cedrus deodara (Roxb.) G. Don in District Solan, Himachal Pradesh, India

Thakur

Tomar

Sharma

2022

IJECC

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Root rot disease is one of the most serious diseases that significantly reduces the plant's productivity, commonly known as collar rot, foot rot, or damping off. The main symptom of this condition is damping off. The newly emerging seedlings collapse and die in areas with severe infection, and the majority of seedlings may die before or after emergence. Due to Root rot disease in C. Deodara which is our state tree, the damage caused by it to the seedlings there was urgent need to identify and study the disease so as to develop proper management strategies. In Himachal Pradesh, a field survey was carried out to know the disease incidence of root rot pathogen in the Cedrus growing regions in district Solan. The survey included the Kanaghat, Solan, and Chail mountains. The Kandaghat range covered Kiari, Karol, and Bisha nurseries, the Solan range Shilli, Nauni nurseries, and the Chail range Chiunth, Chail, and Gaura nurseries were all covered. During the survey of root rot pathogen of Cedrus deodara, 10.90 to 62.15%incidence was recorded in district Solan nurseries. Maximum incidence of the root rot was recorded in Kandaghat range (37.35%) followed by Solan range (33.85%) and Chail range (30.93%), respectively. Amongst nurseries found that,Bisha nursery had maximum incidence (62.15%) followed by Nauni (48.46%) and Chiunth nurseries (44.65), respectively. Cultural and morphological characteristics of root rot pathogen were studied and identified as Rhizoctonia solani. ITS1/ITS4 markers were employed for the molecular identification of the pathogen that caused the root rot in Cedrus deodara, which was confirmed to be binucleate Rhizoctonia AG-E. For the root rot pathogen, isolation frequency was also observed in three solan ranges. Isolation frequency of Chail range comprising of two ranges showed the maximum of 37.71%followed by Kandaghat (34.42%) and Solan (19.37%), respectively. Numerous pathogens were isolated, however, three pathogen, binucleate Rhizoctonia AG-E, Fusarium oxysporum, and Phoma exigua were found to occur most frequently. Binucleate Rhizoctonia AG-E was the most often isolated of the three primary pathogens, occurring at a maximum frequency of 50.23%, followed by P. exigua (18.98%) and F. oxysporum (7.49%). Three different methods were used to test the pathogenicity of binucleate Rhizoctonia AG -E for their ability to produce root rot symptoms. The soil infestation inoculation method recorded the highest disease incidence (83.48%) followed by the root dip (34.53%) and stem application (23.76%), respectively.

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“…Farmers, in Pakistan employ various methods to tackle Globe amaranth weed. They utilize techniques like hand weeding, hoeing, and pulling to control small weeds, although these methods require significant labor (Khan et al, 2021). For farms, manual practices are often combined with approaches.…”

Section: Introductionmentioning

confidence: 99%

Exploring Morphological Traits Variation in Gomphrena Globosa: A Multivariate Analysis

HAIDER,

SAMI,

MAZHAR

et al. 2023

BASRJ

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Gomphrena globosa L. is a weed threatening important crops like wheat, maize, rice, sugarcane, and cotton. It competes with these crops for resources such as water, minerals, and nutrients, reducing crop yield. To address this problem, a study was conducted to examine the characteristics of Gomphrena globosa in three environments. The findings showed a positive relationship between traits studied, including height, weight (dry and fresh), leaf area, leaf length, width, and root length. Interestingly, the plant leaves' width stood out as the factor contributing to its overall height. Location two was identified as favorable for the growth and development of Gomphrena globosa plants. To minimize losses in crop yield caused by this weed species, removing or controlling Gomphrena globosa populations is recommended while emphasizing effective population management techniques.

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Molecular Characterization of Penicillium expansum Isolated from Grapes and its Management by Leaf Extract of Chenopodium murale

Cited by 6 publications

References 28 publications

High throughput biochemical profiling, and functional potential analysis for valorization of grape peduncles

High throughput biochemical profiling, and functional potential analysis for valorization of grape peduncles

Survey, Pathogenicity and Molecular Characterization of Root Pathogens Associated with Root Rot of Cedrus deodara (Roxb.) G. Don in District Solan, Himachal Pradesh, India

Exploring Morphological Traits Variation in Gomphrena Globosa: A Multivariate Analysis

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