Molecular characterization of Pasteurella multocida isolates from swine lungs by Randomly Amplified Polymorphic DNA

Chitarra, Cristiane Silva; Silva, Mayara Inácio Vincenzi da; Brandão, Laila Natasha Santos; Kagueyama, Francielle Cristina; Cândido, Stéfhano Luís; Rosa, Janaina Marcela Assunção; Nakazato, Luciano; Dutra, Valéria

doi:10.1590/0103-8478cr20150153

Cited by 1 publication

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“…Te RAPD clustering performed in this study also supports the results of previous studies [26,31,45]. Te present study's method demonstrates that the M13 primer can be used for genetic diferentiation (DNA fngerprinting) of P. multocida and can thus describe the diversity among P. multocida isolates from Indonesia.…”

Section: Discussionsupporting

confidence: 86%

“…Isolates from Lampung Province and South Sulawesi Province were grouped into two diferent clusters (clusters II and III for Lampung Province; clusters II and IV for South Sulawesi Province). Previous research in Brazil demonstrated that 14 P. multocida isolates sourced from the city of Diamantino could be grouped into two diferent clusters when analyzed using primer M13 [31]. Findings in other countries also indicate that P. multocida originating from one region exhibits high diversity when analyzed using RAPD.…”

Section: Discussionmentioning

confidence: 96%

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Morphology, Biochemical, and Molecular Characterization of Pasteurella multocida Causing Hemorrhagic Septicemia in Indonesia

Desem

Handharyani

Setiyono

et al. 2023

Veterinary Medicine International

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Pasteurella multocida is a Gram-negative bacterium that causes hemorrhagic septicemia (HS) in buffaloes and cattle. The disease causes serious problems in Indonesian livestock and is classified as a serious transmissible animal disease. Previous research has determined the diversity of P. multocida using a serotyping method based on the antigenic properties of capsule polysaccharides. An alternative method for analysis utilizes sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and random amplified polymorphic DNA (RAPD). This study aimed to characterize and determine P. multocida diversity in several regions of Indonesia based on phenotypic character, protein profile, and the band pattern of RAPD results. Bacterial identification was performed using traditional biochemical techniques and API® 20NE systems and then confirmed molecularly using polymerase chain reaction (PCR). The freeze-thawing technique was performed to obtain the bacterial protein extract, and DNA extraction was executed using DNAzol. The extracted protein and RAPD product were then electrophoresed on 12% polyacrylamide gel and 1.5% agarose gel, respectively. The results indicate that the molecular weight range of the protein bands is 12–209 kDa, and the band pattern of the RAPD results ranged from 307–3,100 bp. Based on phenotypical analysis, P. multocida from South Sulawesi Province exhibited a variety of growth characteristics in MacConkey agar media. Using the hierarchical clustering analysis of the band patterns of RAPD and the whole-cell protein profiles, four and five clusters were formed, respectively. These results indicate molecular diversity among P. multocida from several regions of Indonesia.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 96%

Morphology, Biochemical, and Molecular Characterization of Pasteurella multocida Causing Hemorrhagic Septicemia in Indonesia

Desem

Handharyani

Setiyono

et al. 2023

Veterinary Medicine International

View full text Add to dashboard Cite

show abstract

Molecular characterization of Pasteurella multocida isolates from swine lungs by Randomly Amplified Polymorphic DNA

Abstract: Swine respiratory diseases such as atrophic rhinitis and bronchopneumonia caused by Pasteurella (P.) multocida cause important economic losses to the modern swine industry. The purpose of this study was to characterize P. multocida strains isolated from swine lungs by RAPD (Randomly

Cited by 1 publication

References 21 publications

Morphology, Biochemical, and Molecular Characterization of Pasteurella multocida Causing Hemorrhagic Septicemia in Indonesia

Morphology, Biochemical, and Molecular Characterization of Pasteurella multocida Causing Hemorrhagic Septicemia in Indonesia

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