Molecular characterization of Pacific white shrimp ( Litopenaeus vannamei ) sodium bicarbonate cotransporter (NBC) and its role in response to pH stress

Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development

“…After acclimation in artificial seawater (30 ppt and pH 8.2) at 28°C for 2 weeks, fifty-five shrimp were randomly transferred into nine independent 20-L tanks (6 individuals per tank). The challenge conditions for shrimp included low temperature (13°C) and high temperature (37°C) [as validated by (Huang et al, 2017)], low pH (pH 6.8) and high pH (pH 8.9) [as validated by (Cai et al, 2017)], and low salinity (10 ppt) and high salinity (45 ppt) [as validated by (Chen et al, 2016b)]. Six shrimp from each group were killed at 6 h after challenge.…”

“…The effects of HSP40 on ovarian development were investigated using an RNAi approach. Small interfering RNA (siRNA) targeting sites were identified using the Ambion siRNA target finder program, and the selected sequences were subjected to global BLAST against non-redundant nucleotide sequences, as described previously (Cai et al, 2017). Three sets of siRNA (HSP40-42, HSP40-474, and HSP40-825, Supplementary Data 3) specific against Lv-HSP40 and the non-targeting nematode siRNA (NC-siRNA) were synthesized by Sangon Biotech Company.…”

“…Previtellogenic female shrimp with body sizes as described above were used for RNAi experiments. The injection of siRNA was conducted as described before (Cai et al, 2017). Briefly, one-hundred fifty shrimp were transferred randomly into three independent 1-m 3 tanks (fifty individuals per tank).…”

“…The Pacific white shrimp Litopenaeus vannamei is the most widely cultured and productive crustacean species, nearly 71% of the global economic penaeid shrimp production (Alfaro-Montoya, 2010). However, the development of the L. vannamei aquaculture industry in recent years has been seriously affected by various harsh culture environmental parameters, such as water temperature (Huang et al, 2017), acidity/alkalinity (Lemonnier et al, 2004; Cai et al, 2017), salinity (McGraw and Scarpa, 2004; Chen et al, 2016b), nutrition (Romano and Zeng, 2012) and disease exposure (Chen et al, 2016a; Tian et al, 2018a,b). The reproductive performance of shrimp is currently regarded as another major drawback in L. vannamei aquaculture (Alfaro-Montoya, 2010; Chen et al, 2014; Luo et al, 2015).…”

Comprehensive identification and profiling of Chinese shrimp (Fenneropenaeus chinensis) microRNAs in response to high pH stress using Hiseq2000 sequencing

“…It is well known that acid/base homeostasis is primarily regulated by ion transport proteins. The sodium bicarbonate cotransporter, an integral membrane ion transporter, was significantly up‐regulated both in low and high pH challenged Litopenaeus vannamei (Cai et al, ). Many studies have revealed that carbonic anhydrase (CA) plays a central role in branchial ion transport in crustaceans.…”