Molecular characterization of new members of the Hevea brasiliensis hevein multigene family and analysis of their promoter region in rice

Pujade-Renaud, Valérie; Sanier, Christine; Cambillau, Laurence; Pappusamy, Arokiaraj; Jones, Heddwyn; Ruengsri, Natsuang; Tharreau, Didier; Chrestin, Hervé; Montoro, Pascal; Narangajavana, Jarunya

doi:10.1016/j.bbaexp.2004.12.013

Cited by 23 publications

(22 citation statements)

References 28 publications

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“…These genes are highly conserved in their transcribed region but highly divergent in their regulatory sequences upstream the position -40 from the transcription start. Functional analysis of the HEV2.1 gene promoter in fusion with the gusA reporter gene in transgenic rice revealed GUS activity in leaf blades, root vascular tissues and in pollen, and additional up-regulation by wounding and pathogen infection in leaves (Pujade-Renaud et al 2005). These results were in agreement with previously described wound-induced overexpression of the hevein genes in rubber tree (Broekaert et al 1990) and the proposed role of hevein in plant defence (d'Auzac et al 1995).…”

Section: Introductionsupporting

confidence: 89%

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Expression of the HEV2.1 gene promoter in transgenic Hevea brasiliensis

Montoro

Lagier

Baptiste

et al. 2008

Plant Cell Tiss Organ Cult

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Hevein is involved in the coagulation of rubber particles and is known to be highly expressed in latex cells from Hevea brasiliensis. As all HEVEIN genes are highly conserved in their transcribed region, a promoter functional analysis was carried out in transgenic rubber tree in order to evaluate the expression of HEV2.1, one member of the HEVEIN multigenic family. Three out of the 14 established independent transgenic lines carrying the HEV2.1:: GUS construct were selected and set to regenerate plantlets. In situ hybridization of transgenic and wildtype plant tissues was respectively carried out with gusA and HEVEIN antisense mRNA probes. This showed co-localized-expression of the HEV2.1 gene with the overall HEVEIN gene expression. Laticiferspecific expression was observed in roots and stems, but not in leaves where HEVEIN genes were expressed in all cell types. Since HEV2.1::GUS callus lines responded significantly to light stimulation, up-regulation of the expression of HEV2.1 genes by light was suggested in leaves.

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Section: Introductionsupporting

confidence: 89%

“…Hevein is an acidic protein belonging to the lectin super-family and is encoded by a multigene family consisting of at least five members (Pujade-Renaud et al 2005). These genes are highly conserved in their transcribed region but highly divergent in their regulatory sequences upstream the position -40 from the transcription start.…”

Section: Introductionmentioning

confidence: 99%

Expression of the HEV2.1 gene promoter in transgenic Hevea brasiliensis

Montoro

Lagier

Baptiste

et al. 2008

Plant Cell Tiss Organ Cult

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“…These motifs were initially found in the promoter of rolD genes, which are preferentially expressed in roots compared to leaves, and more specifically in the root elongation zone and vascular tissue. ATATT-motifs were also found in two promoters from Hevea brasiliensis that showed an expression in vascular tissues of roots in transgenic rice (Pujade-Renaud et al, 2005). A root-specific regulatory element was also identified in the Mec1 promoter, such as an SPBF-binding site, required by sporamin gene expression, a major protein in tuberous roots of Ipomea batatas Nakamura, 1992, 1994).…”

Section: In Silico Analysis Of the Mec1 Promoter Sequencementioning

confidence: 92%

“…In silico analysis has been used with success in the identification of putative regulatory elements in plant promoters (Wei-Min et al, 2005;Pujade-Renaud et al, 2005). In order to identify putative transcription factor binding sites and conserved plant cis-acting regulatory elements, the 952-bp Mec1 promoter sequence was analyzed using TFSearch algorithm and PlantCARE and PLACE databases.…”

Section: In Silico Analysis Of the Mec1 Promoter Sequencementioning

confidence: 99%

Isolation and characterization of the promoter sequence of a cassava gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in storage roots

Souza

Fj²,

Ec³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. Cassava is one of the most important tropical food crops for more than 600 million people worldwide. Transgenic technologies can be useful for increasing its nutritional value and its resistance to viral diseases and insect pests. However, tissuespecific promoters that guarantee correct expression of transgenes would be necessary. We used inverse polymerase chain reaction to isolate a promoter sequence of the Mec1 gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in cassava storage roots. In silico analysis revealed putative cis-acting regulatory elements within this promoter sequence, including root-specific elements that may be required for its expression in vascular tissues. Transient expression experiments showed that the Mec1 promoter is functional, since this sequence was able to drive GUS expression in bean embryonic axes. Results from our computational analysis can serve as a guide for functional experiments to identify regions with tissue-specific Mec1 promoter activity. The DNA sequence that we identified is a new promoter that could be a candidate for genetic engineering of cassava roots.

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“…( , 2005 also cloned and characterized the same gene from Indian Hevea clones and demonstrated prolonged accumulation of b -1,3-glucanase transcripts in abnormal leaf falltolerant RRII 105. Hevein, a lectin-like protein, belonging to a multigene family was found to play a crucial role in the protection of wound sites from fungal attack through latex coagulation (Broekaert et al 1990 ;Pujade-Renaud et al 2005 ) . Overexpression of chitinase was noticed during fungal infection and by ethylene stimulation.…”

Section: Defense/stress-related Genesmentioning

confidence: 99%

Genomics of Hevea Rubber

Saha

Priyadarshan

2012

Genomics of Tree Crops

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Hevea brasiliensis is the most recent domesticated tree species from Amazonian rain forest producing latex of commercial utility. Major hurdles for genetic improvement of rubber tree were attributed to its perennial nature, long juvenile period, and a narrow genetic base. Further, the limited availability of Hevea genomic resources/information is another impediment to genomics-assisted crop improvement. Improvement of rubber tree in terms of latex production through breeding was the major focus of the scienti fi c community dealing with the crop. Due to unidirectional selection for yield, other secondary attributes of rubber plants were lost during the process of developing high-yielding clones.

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Molecular characterization of new members of the Hevea brasiliensis hevein multigene family and analysis of their promoter region in rice

Cited by 23 publications

References 28 publications

Expression of the HEV2.1 gene promoter in transgenic Hevea brasiliensis

Expression of the HEV2.1 gene promoter in transgenic Hevea brasiliensis

Isolation and characterization of the promoter sequence of a cassava gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in storage roots

Genomics of Hevea Rubber

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