Molecular characterization of mutations induced by gamma irradiation in rice

Morita, Ryouhei; Kusaba, Makoto; Iida, Shūichi; Yamaguchi, Hiroyasu; Nishio, Takeshi; Nishimura, Masaharu

doi:10.1266/ggs.84.361

Cited by 119 publications

(101 citation statements)

References 51 publications

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“…1A and 3; Supplementary Fig. S1) in comparison to deletions that are induced by γ-rays (0%, Morita et al, 2009) and heavy ions with lower LETs (0% Fig. 3.…”

Section: Complex Rearrangement Induced By Fe-ion Irradiationmentioning

confidence: 98%

Effect of high-LET Fe-ion beam irradiation on mutation induction in <i>Arabidopsis thaliana</i>

Kazama¹,

Hirano²,

Nishihara

et al. 2013

Genes Genet. Syst.

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Heavy-ion beams are powerful mutagens. They cause a broad spectrum of mutation phenotypes with high efficiency even at low irradiation doses and short irradiation times. These mutagenic effects are due to dense ionisation in a localised region along the ion particle path. Linear energy transfer (LET; keV·μm -1 ), which represents the degree of locally deposited energy, is an important parameter in heavy-ion mutagenesis. For high LET radiation above 290 keV•μm -1 , however, neither the mutation frequency nor the molecular nature of the mutations has been fully characterised. In this study, we investigated the effect of Fe-ion beams with an LET of 640 keV•μm -1 on both the mutation frequency and the molecular nature of the mutations. Screening of well-characterised mutants (hy and gl) revealed that the mutation frequency was lower than any other ion species with low LET. We investigated the resulting mutations in the 4 identified mutants. Three mutants were examined by employing PCR-based methods, one of which had 2-bp deletion, another had 178 bp of tandemly duplication, and other one had complicated chromosomal rearrangements with variable deletions in size at breakpoints. We also detected large deletions in the other mutant by using array comparative genomic hybridisation. From the results of the analysis of the breakpoints and junctions of the detected deletions, it was revealed that the mutants harboured chromosomal rearrangements in their genomes. These results indicate that Fe-ion irradiation tends to cause complex mutations with low efficiency. We conclude that Fe-ion irradiation could be useful for inducing chromosomal rearrangements or large deletions.

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“…1A and 3; Supplementary Fig. S1) in comparison to deletions that are induced by γ-rays (0%, Morita et al, 2009) and heavy ions with lower LETs (0% Fig. 3.…”

Section: Complex Rearrangement Induced By Fe-ion Irradiationmentioning

confidence: 98%

Effect of high-LET Fe-ion beam irradiation on mutation induction in <i>Arabidopsis thaliana</i>

Kazama¹,

Hirano²,

Nishihara

et al. 2013

Genes Genet. Syst.

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“…On the other hand, the absence of 26 kDa globulin induced the collapse of PB-II structures in K × 433. Lgc1 and glb1 have deletions of a glutelin gene and the 26 kDa globulin gene, respectively (Kusaba et al 2003, Morita et al 2007). Glutelin and globulin are components of PB-II (Yamagata et al 1982), but the influence of their reduction on PB structure was different.…”

Section: Ultrastructure Of Protein Bodies During Seed Developmentmentioning

confidence: 99%

“…K × 433 is derived from a backcross of Koshihikari and the mutant line 89WPKG30-433 (Ashida et al 2006, Iida et al 1998 and shows the lack of a 26 kDa globulin, which is controlled by a single recessive gene, glb1. Morita et al (2007) reported that the glb1 mutant has a 63 kb deletion in a region including the 26 kDa globulin gene.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of protein bodies in mutant rice (Oryza sativa L.) with altered storage protein composition

et al. 2011

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Many rice mutants with altered storage protein composition, which relate to protein body II (PB-II), have been produced in order to obtain rice with new cooking properties or lowered protein digestibility, but the PB structures in the endosperm of such mutants remain obscure. We examined the polypeptide composition and ultrastructure of the endosperm during seed development using rice mutants with the low-glutelin-content gene (Lgc1) and/or 26 kDa globulin deficient gene (glb1). Lgc1 and glb1 have deletions of a region including glutelin structural genes and 26 kDa globulin gene, respectively. Mutants with Lgc1 accumulated prolamin polypeptides from an early stage of seed development, and numerous intact PB-I and a few, small, chipped PB-II were observed in the endosperm. The glb1 mutant showed deformed PB-II structures, which had similar electron density to wild-type PB-II. Both glutelins and globulins accumulate in PB-II, but the influence of the change in their composition on PB-II structure would be different. The unique PB-II structure in the glb1 mutant may bring about new processing properties for rice.

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“…Gamma irradiation results in small deletions (1-10 bp) while neutrons cause 300 bp to 12 kbp deletions and chemical mutagens result in point mutations mainly G/C-to-A/T transitions (Morita et al, 2009). On the other hand, ion beams have high linear energy transfer (LET) ranging from 22.5 keV μm -1 to 4000 keV μm -1 compared to 0.2 to 2 keV μm 1 LET of γ-rays and X-rays (Ryuto et al, 2008).…”

Section: Developing 'Mutant' Resourcesmentioning

confidence: 99%

Mutant Resources and Mutagenomics in crop plants

Suprasanna

Jain

2017

Emir J Food Agric

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Agricultural sustainability and food security are major challenges facing continued population growth. Integration of existing and new technologies for the induction and exploitation of genetic diversity towards developing healthier, nutritious and productive crops is the need of the hour. Mutagenesis is a proven technology for the development of improved or novel varieties with desirable traits. Several mutant genes have been successfully explored, either directly or indirectly, to complement crop productivity. The advent of genomics approaches and plant genome sequencing has benefitted mutation discovery and mutant characterization. Plant mutant repositories are being established to serve as platforms for basic and applied research in crop improvement. This review briefly outlines the impact and molecular/genomic characterization of induced mutations in crop improvement.

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Molecular characterization of mutations induced by gamma irradiation in rice

Cited by 119 publications

References 51 publications

Effect of high-LET Fe-ion beam irradiation on mutation induction in <i>Arabidopsis thaliana</i>

Effect of high-LET Fe-ion beam irradiation on mutation induction in <i>Arabidopsis thaliana</i>

Ultrastructure of protein bodies in mutant rice (Oryza sativa L.) with altered storage protein composition

Mutant Resources and Mutagenomics in crop plants

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