Molecular characterization of multidrug-resistant Escherichia coli of the phylogroups A and C in dairy calves with meningitis and septicemia

Uriarte, Enrique Leopoldo Louge; Pasayo, Ramón A. González; Massó, Mariana; Páez, Laura C. Carrera; Moncla, Manuel Domínguez; Donis, Nicolás; Malena, Rosana; Méndez, Alejandra; Morrell, Eleonora; Giannitti, Federico; Armendano, Joaquín I.; Faverín, Claudia; Centrón, Daniela; Parreño, Viviana; Odeón, Anselmo; Quiroga, María Paula; Moreira, Ana Rita Silva

doi:10.1016/j.micpath.2021.105378

Cited by 4 publications

(6 citation statements)

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“…In another study, researchers found that 7% of calves with bacteremia symptoms showed growth from blood cultures, and 20% of the positive blood cultures were identified as E. coli [35]. However, in another study on this subject, researchers emphasized that 80% of septicemia was caused by E. coli [37]. These researchers reported that in addition to E. coli, Salmonella spp., Enterobacter aerogenes, Campylobacter fetus, Klebsiella spp., Streptococcus dysgalactiae, and Trueperella pyogenes were isolated from blood cultures of septicemic calves [35,37].…”

Section: Resultsmentioning

confidence: 99%

“…However, in another study on this subject, researchers emphasized that 80% of septicemia was caused by E. coli [37]. These researchers reported that in addition to E. coli, Salmonella spp., Enterobacter aerogenes, Campylobacter fetus, Klebsiella spp., Streptococcus dysgalactiae, and Trueperella pyogenes were isolated from blood cultures of septicemic calves [35,37].…”

Section: Resultsmentioning

confidence: 99%

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Microbiological characterization and genetic analysis of bacteria isolated from blood cultures and fecal samples in calves with symptoms of septicemia and diarrhea

Uslu,

Sayin,

Balevi

et al. 2024

RC FCV-LUZ

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Diarrhea in calves can be caused by bacteria, viruses, and parasites. Among bacteria, Escherichia coli is considered responsible for the appearance of enteric diarrhea and septicemia in these animals, conditions that require immediate attention. Among E. coli infections of calves, more focus is placed on intestinal pathogenic (InPEC) infections, and extra – intestinal pathogenic (ExPEC) infections are ignored. This study aims to reveal which E. coli pathotype causes the infection as molecular and serotype and to reveal the differences according to the age groups of the factors in the herd. Blood and fecal samples of 10 calves aged 3 – 15 d with diarrhea were analyzed. The primary agent causing enteritis was determined by examining the stool samples with BoviD – 5 Ag. Then, samples were subjected to culture and identification processes. It was determined that the stool samples had 2/10 with E. coli K99, 4/10 with rotavirus, and 4/10 with mixed rotavirus infections and Cryptosporidium spp. E. coli was detected from all blood samples by hemoculture. The study isolated only the SepEC and ETEC groups from samples. All SepEC isolates were determined to carry type 1 pilus responsible for adhesion. In addition, it was determined that 9/10 of the SepEC group carried the colicin V gene responsible for pathogenicity. Also, all E. coli isolated from calves aged 3 – 15 d were found to be resistant to antibiotics. In conclusion, primary enteritis is caused by rotavirus Cryptosporidium spp. and ETEC. However, it was determined that SepEC group E. coli causing septicemia showed different antigenic and genetic features than E. coli in the intestinal tract. The virulence factors of the SepEC group may vary due to genomic plasticity, and their antigenic structures should be more closely examined and added to vaccine test studies.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Microbiological characterization and genetic analysis of bacteria isolated from blood cultures and fecal samples in calves with symptoms of septicemia and diarrhea

Uslu,

Sayin,

Balevi

et al. 2024

RC FCV-LUZ

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“…Es muy importante determinar los genes que están involucrados en estos procesos de resistencia relacionados a la utilización de estos fármacos, como genes de adhesión, transportadores de hierro (33,34) , como es el caso del gen iucD (9,35) , además, los procesos de transferencia horizontal de genes, la presión de selección, que provoca el uso indiscriminado de antibióticos (36,37) , todo lo mencionado es conocimiento necesario para evaluar los procesos de tratamiento y control de diarrea en terneros en la región de Cajamarca.…”

Section: Discussionunclassified

“…Las terneras eliminan frecuentemente microorganismos a través de sus heces, generando la diseminación de bacterias en el ambiente de la granja, lo que podría causar infecciones en los demás animales. Se han obtenido aislamientos de E. coli provenientes de heces de terneras lecheras que presentan resistencia a múltiples antibióticos del grupo de las fluoroquinolonas y se determinó el gen iucD como el más prevalente (9) . Asimismo, en otros estudios se menciona sobre el operón de aerobactina (iucD), que produce cuatro tipos de sideróforos: enterobactina, salmoquelina, aerobactina y yersiniabactina.…”

Section: Introductionunclassified

“…Los genes involucrados en la biosíntesis de sideróforos son encontrados en cepas uropatógenas (UPEC) y cepas comensales; sin embargo, salmoquelina, aerobactina y yersiniabactina son localizados en islas de patogenicidad asociados a UPEC, pero no en cepas comensales, sugiriendo que los sistemas de captación de hierro fueron adquiridos por transferencia horizontal de genes. La aerobactina es un sideróforo presente en la mayoría de las cepas UPEC, teniendo una gran estabilidad en la unión al Fe 3+ y es uno de los encargados en el secuestro de hierro durante una infección del tracto urinario (ITU), la combinación de genes de adherencia/captación de hierro/toxicidad muestra la elevada virulencia y el potencial de daño que poseen las cepas para causar una ITU (9,10) .…”

Section: Introductionunclassified

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Evaluación de resistencia a antibióticos en muestras de heces de terneros con diarrea en la región Cajamarca, Perú

Cabrera González,

Vásquez Pérez,

Quilcate-Pairazamán

et al. 2023

Rev. Mex. Cienc. Pecu.

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La diarrea está asociada con bacterias infecciosas que ocasionan mortalidad en terneros como Escherichia coli, representando un problema para los productores de leche y carne a nivel global, provocando grandes pérdidas económicas. En este estudio se evaluó la resistencia a cepas de E. coli aisladas de heces diarreicas de terneros recién nacidos de la región Cajamarca. Se recolectaron 52 muestras de heces de terneros de cinco provincias de la región Cajamarca para el aislamiento de E. coli en agar MacConkey con sorbitol. La identificación molecular de E. coli se realizó mediante la amplificación del gen uidA por PCR convencional y luego se evaluó la susceptibilidad/resistencia a antibióticos utilizando la metodología de Kirby-Bauer y el uso de discos de antibiótico con neomicina, tetraciclina, sulfametoxazol-trimetroprim y enrofloxacina. Los resultados fueron que el 96.15 % de cepas de E. coli fueron resistentes a tetraciclina, el 51.92 % a sulfametropim, el 26.92 % a neomicina y el 9.61 % a enrofloxacina. También se demostró que el 30.76 % presentaban resistencia a dos fármacos, el 19.23 % a tres fármacos y el 5.76 % a cuatro fármacos; se encontró diferencia significativa de resistencia a tetraciclina (P<0.0001). Se concluye que los terneros neonatos de la región Cajamarca que presentaban diarrea son portadores de E. coli resistentes a antibióticos, representando un problema para los criadores de ganado vacuno, ya que estas cepas pueden causar la muerte de los animales y contribuyen a la diseminación de la resistencia de antibióticos.

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Investigation of virulence factors, phylogenetic grouping, multiple-locus variable number tandem repeat analysis, and antimicrobial susceptibility of E. coli isolated from aborted bovine fetal tissue

Özgen,

Yanmaz,

Bağatir

2023

Letters in Applied Microbiology

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Escherichia coli is an important microorganism for cattle breeding. The aim of this study was to investigate the presence of phylogenetic groups, virulence factors, genotyping with multi-locus variable tandem repeat analysis (MLVA), and susceptibility to commonly used antimicrobial agents in E. coli strains isolated from aborted bovine fetal samples. In this study, phylogrouping and various virulence genes were analyzed by PCR in E. coli strains isolated from 637 bovine fetal tissue samples. Consequently, E. coli was isolated and identified in 24 samples in culture. Of the 24 isolates identified as positive, 12.5% were defined as group A, 83.3% as B1, and 4.2% as group B2. Of the E. coli isolates, virulence factor fimH was identified in eight (33.3%), traT in 15 (62.5%), ompT in five (20.8%), CNF1 in one (4.16%), and CNF2 in six (25%). Seven genotypic groups were determined as a result of the analysis with the MLVA 10 method. According to the antimicrobial susceptibility test results, high resistance was determined against amoxicillin/clavulanic acid and oxytetracycline. In conclusion, strains of E. coli containing CNF1, CNF2, fimH, traT, and ompT virulence factors can be associated with bovine abortions. It is noteworthy that the dominant phylogenetic group B1 has been observed in cases of cattle abortions.

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Molecular characterization of multidrug-resistant Escherichia coli of the phylogroups A and C in dairy calves with meningitis and septicemia

Cited by 4 publications

References 89 publications

Microbiological characterization and genetic analysis of bacteria isolated from blood cultures and fecal samples in calves with symptoms of septicemia and diarrhea

Microbiological characterization and genetic analysis of bacteria isolated from blood cultures and fecal samples in calves with symptoms of septicemia and diarrhea

Evaluación de resistencia a antibióticos en muestras de heces de terneros con diarrea en la región Cajamarca, Perú

Investigation of virulence factors, phylogenetic grouping, multiple-locus variable number tandem repeat analysis, and antimicrobial susceptibility of E. coli isolated from aborted bovine fetal tissue

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