Molecular Characterization of Mosquitoes (Diptera: Culicidae) in Northwestern Iran by Using rDNA-ITS2

Khoshdel-Nezamiha, Farahnaz; Vatandoost, Hassan; Oshaghi, Mohammad Ali; Azari-Hamidian, Shahyad; Mianroodi, Reza Arabi; Dabiri, Farrokh; Bagheri, Masoomeh; Terenius, Olle; Chavshin, Ali Reza

doi:10.7883/yoken.jjid.2015.269

Cited by 20 publications

(12 citation statements)

References 32 publications

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“…To overcome these impediments, sequencing of the COI mtDNA barcode region of organisms, particularly mosquitoes, is now widely used (Hebert et al, 2003a,b;Cywinska et al, 2006;Kumar et al, 2007;Gunay et al, 2015;Versteirt et al, 2015). In addition, ribosomal genes such as the internal transcribed spacer (ITS2) sequence of ribosomal DNA is also used in identification of mosquito species, either independently or in combination with COI (Linton et al, 2001(Linton et al, , 2005Danabalan et al, 2012;Khoshdel-Nezamiha et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Molecular approaches for blood meal analysis and species identification of mosquitoes (Insecta: Diptera: Culicidae) in rural locations in southern England, United Kingdom

Hernández‐Triana

Brugman

Prosser

et al. 2017

Zootaxa

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Thirty-four species of Culicidae are present in the UK, of which 15 have been implicated as potential vectors of arthropod-borne viruses such as West Nile virus. Identification of mosquito feeding preferences is paramount to the understanding of vector-host-pathogen interactions which, in turn, would assist in the control of disease outbreaks. Results are presented on the application of DNA barcoding for vertebrate species identification in blood-fed female mosquitoes in rural locations. Blood-fed females (n = 134) were collected in southern England from rural sites and identified based on morphological criteria. Blood meals from 59 specimens (44%) were identified as feeding on eight hosts: European rabbit, cow, human, barn swallow, dog, great tit, magpie and blackbird. Analysis of the cytochrome c oxidase subunit I mtDNA barcoding region and the internal transcribed spacer 2 rDNA region of the specimens morphologically identified as Anopheles maculipennis s.l. revealed the presence of An. atroparvus and An. messeae. A similar analysis of specimens morphologically identified as Culex pipiens/Cx. torrentium showed all specimens to be Cx. pipiens (typical form). This study demonstrates the importance of using molecular techniques to support species-level identification in blood-fed mosquitoes to maximize the information obtained in studies investigating host feeding patterns.

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Section: Introductionmentioning

confidence: 99%

Molecular approaches for blood meal analysis and species identification of mosquitoes (Insecta: Diptera: Culicidae) in rural locations in southern England, United Kingdom

Hernández‐Triana

Brugman

Prosser

et al. 2017

Zootaxa

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“…From 2013, the guideline for susceptibility test was modified, 90% mortality indicating the resistance, so that the results of previous years indicated the susceptible strains resistant to insecticides. abundance, diurnal activity and man-biting behavior [31][32][33][34][35][36][37][38][39][40].…”

Section: Resultsmentioning

confidence: 99%

Ecology, monitoring and mapping of insecticide resistance of malaria vector, Anopheles culicifacies (Diptera: Culicidae) to different imagicides in Iran

Vatandoost¹,

Hanafi‐Bojd²,

Ahmad³

et al. 2016

Asian Pac J Trop Dis

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“…longiareolata ) in the study area. Previous studies have also identified these mosquito species in the northwest of Iran [ 43 – 46 ].…”

Section: Main Textmentioning

confidence: 99%

“…Given the role of this species in the transmission of some important diseases and its widespread presence in different parts of the world, including the northwestern region [ 34 , 43 – 46 ] and West Azerbaijan Province (which shares border with four countries) in Iran, the symbiotic bacteria of Culiseta longiareolata were investigated in this study. In the present study, the cultivable bacterial symbionts of Culiseta longiareolata were isolated, cultivated and identified using 16S rRNA fragment analysis.…”

Section: Introductionmentioning

confidence: 99%

Identification of symbiotic bacteria in the midgut of the medically important mosquito, Culiseta longiareolata (Diptera: Culicidae)

2020

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Objective The potential use of symbiotic bacteria for the control of mosquito-borne diseases has attracted the attention of scientists over the past few years. Culiseta longiareolata is among the medically important mosquitoes that transmit a wide range of vector-borne diseases worldwide. However, no extensive studies have been done on the identification of its symbiotic bacteria. Given the role of this species in the transmission of some important diseases and its widespread presence in different parts of the world, including northwestern parts and the West Azerbaijan Province in Iran, a knowledge about the symbiotic bacteria of this species may provide a valuable tool for the biological control of this mosquito. Accordingly, the present study was conducted to isolate and identify the cultivable isolates bacterial symbionts of Culiseta longiareolata using 16S rRNA fragment analysis. Results The midguts of 42 specimens of Cs. longiareolata were dissected, and the bacteria were cultured on agar plates. After the purification of the bacterial colonies, 16srRNA region amplification and gene sequence analysis were performed, and the sequences were confirmed by biochemical methods. In the present study, 21 isolates belonging to the genera Acinetobacter , Aerococcus , Aeromonas , Bacillus , Carnobacterium , Klebsiella , Morganella , Pseudomonas , Shewanella and Staphylococcus were identified.

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Molecular Characterization of Mosquitoes (Diptera: Culicidae) in Northwestern Iran by Using rDNA-ITS2

Cited by 20 publications

References 32 publications

Molecular approaches for blood meal analysis and species identification of mosquitoes (Insecta: Diptera: Culicidae) in rural locations in southern England, United Kingdom

Molecular approaches for blood meal analysis and species identification of mosquitoes (Insecta: Diptera: Culicidae) in rural locations in southern England, United Kingdom

Ecology, monitoring and mapping of insecticide resistance of malaria vector, Anopheles culicifacies (Diptera: Culicidae) to different imagicides in Iran

Identification of symbiotic bacteria in the midgut of the medically important mosquito, Culiseta longiareolata (Diptera: Culicidae)

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