Molecular characterization of microorganisms with industrial potential for methane production in sludge from Kangemi sewage treatment plant, Nyeri county–Kenya

Kimisto, K. Allan; Muia, Anastasia W.; Ong'ondo, Geoffrey Odhiambo; Kimani, N. Cyrus

doi:10.1016/j.heliyon.2023.e15715

Cited by 3 publications

(6 citation statements)

References 29 publications

(36 reference statements)

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“…Meanwhile, at thermophilic temperature (T0), the RA of archaea above 5% were Methanothermobacter (48.5%), Methanoculleus (15.9%), Methanosaeta (14.7%) and Methanospirillum (5.0%). Methanosaeta was highly adaptable to anaerobic environments, which could utilize a wide range of organic substances as substrates for growth and methanogenesis [57]. Methanobacterium was a hydrogenotrophic methanogen that could interact with other methanogens and fermenters to promote the conversion of organic matter [58].…”

Section: Archaea Communitymentioning

confidence: 99%

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Effects of One-Step Abrupt Temperature Change on Anaerobic Co-Digestion of Kitchen Waste with Dewatered Sludge

Hu,

Zhou,

Zhu

et al. 2023

Fermentation

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The operating temperature of anaerobic digesters should be adjusted to adapt to seasonal variations in environmental temperature and the composition of organic solid waste. This study investigated the effects of one-step abrupt temperature changes (from mesophilic to thermophilic temperature, M–T, and from thermophilic to mesophilic temperature, T–M) and the inoculation ratio on methane yield and microbial diversity during the anaerobic co-digestion of kitchen waste with dewatered sludge. The results showed that the cumulative methane yield (CMY) level resulting from thermophilic control and the M–T digesters was greater than that resulting from mesophilic control and the T–M digesters. The CMF of M–T digesters increased, whereas the CMY of T–M digesters gradually decreased with an increase in the inoculation ratio. The maximal CMY was 385.1 mL/g-VSSadded, which corresponded to an M–T digester with a 5% inoculation ratio. In the later stage of anaerobic digestion, the bacterial community of T–M was more diverse than that of M–T, but the archaeal community of M–T was more diverse than that of T–M. The one-step temperature change from thermophilic to mesophilic temperature was more stable than that from mesophilic to thermophilic temperature.

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Section: Archaea Communitymentioning

confidence: 99%

“…Methanofollis was a hydrogenotrophic methanogen with an optimum growth temperature of 37 • C [25]. Methanosarcina was an acetic acid-nutrient methanogen that typically grew at mesophilic temperatures [57]. Methanothermobacter was a hydrogenotrophic methanogen at thermophilic temperatures [59].…”

Section: Archaea Communitymentioning

confidence: 99%

Effects of One-Step Abrupt Temperature Change on Anaerobic Co-Digestion of Kitchen Waste with Dewatered Sludge

Hu,

Zhou,

Zhu

et al. 2023

Fermentation

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“…These acids combine with alkali metal ions in the reactor to produce the corresponding salts. The conversion of VFA to methane via methanogenic bacteria consumes hydrogen ions and may result in an increase in pH [27].…”

Section: Ph Tan and Fanmentioning

confidence: 99%

“…Moreover, Methanothermobacter is also very effective in degrading proteins [52], which is beneficial to sludge anaerobic digestion (proteins are the main component of sludge organic matter). Methanosarcina typically grows at temperatures in the range of 30-45 • C, with some strains able to grow at a wider range of temperatures [27]. Methanosarcina is an acetic acid-nutrient methanogenic archaea, which means they utilize acetic acid as a substrate for growth and methanogenesis [47].…”

Section: Archaea Distributionmentioning

confidence: 99%

Comparison of Thermophilic–Mesophilic and Mesophilic–Thermophilic Two-Phase High-Solid Sludge Anaerobic Digestion at Different Inoculation Proportions: Digestion Performance and Microbial Diversity

Wang,

et al. 2023

Microorganisms

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This study investigated the performance of thermophilic–mesophilic (T-M) and mesophilic–thermophilic (M-T) two-phase sludge anaerobic digestion at different inoculation proportions after a change in digestion temperature. After temperature change, the pH, total ammonia nitrogen (TAN), free ammonia nitrogen (FAN), solubility chemical oxygen demand (SCOD), and total alkalinity (TA) levels of two-phase digesters were between thermophilic control digesters and mesophilic control digesters. However, the volatile fatty acid (VFA) levels of two-phase digesters were higher than those of thermophilic or mesophilic control digesters. The bacteria communities of M-T two-phase digesters were more diverse than those of T-M. After a change in digestion temperature, the bacterial community was dominated by Coprothermobacter. After a change of digestion temperature, the relative abundance (RA) of Methanobacterium, Methanosaeta, and Methanospirillum of M-T two-phase digesters was higher than that of T-M two-phase digesters. In comparison, the RA of Methanosarcina of T-M two-phase digesters was higher than that of M-T two-phase digesters. The ultimate methane yields of thermophilic control digesters were greater than those of mesophilic control digesters. Nevertheless, the ultimate methane yield levels of M-T two-phase digesters were greater than those of T-M two-phase digesters. The ultimate methane yields of all two-phase digesters presented an earlier increase and later decrease trend with the increasing inoculation proportion. Optimal methane production condition was achieved when 15% of sludge (T-M15) was inoculated under mesophilic–thermophilic conditions, which promoted 123.6% (based on mesophilic control) or 27.4% (based on thermophilic control). An optimal inoculation proportion (about 15%) balanced the number and activity of methanogens of high-solid sludge anaerobic digestion.

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“…Methanothermobacter have been shown to be involved in the hydrogenotrophic route (i.e., they use CO2 and H2 to produce CH4) [66,69].…”

Section: Anaerobic Digestion Status and Current Limitationsmentioning

confidence: 99%

Microbial electromethanogenesis for co2 valorisation and electrical energy storage

Carrillo Peña

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Anion exchange membranes Arc Archaea Bact Bacteria BCGO Biotic cathode modified with graphene oxide BCUM Biotic cathode unmodified BES Bioelectrochemical systems BPM Bipolar membranes C1 Electrode Capacitance CA Chronoamperometry CA Chemical absorption CBIO or C2 Biofilm capacitance CCU Carbon capture and utilisation CCUS Carbon capture, utilisation, and storage CE Coulombic (or current) efficiency CEM Cation exchange membranes CF Carbon felt CHP Combined heat and power CO2 Carbon dioxide COD Chemical oxygen demand CPE Constant phase element CV Cyclic voltammetry CW Cheese Whey DEVS Diluted exhausted vine shoot fermentation broth DIET Direct interspecies electron transfer EDL Electrical double layer EEC Electrical equivalent circuit EET Extracellular electron transfer EIS Electrochemical impedance spectroscopy LIST OF ABBREVIATIONS VFAs Volatile fatty acids VS Volatile solids Wd or W Warburg element WS Water scrubbing WWTP Wastewater treatment plants ηQc Charge storage efficiency ρf Faradaic component ρi Current density

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Molecular characterization of microorganisms with industrial potential for methane production in sludge from Kangemi sewage treatment plant, Nyeri county–Kenya

Cited by 3 publications

References 29 publications

Effects of One-Step Abrupt Temperature Change on Anaerobic Co-Digestion of Kitchen Waste with Dewatered Sludge

Effects of One-Step Abrupt Temperature Change on Anaerobic Co-Digestion of Kitchen Waste with Dewatered Sludge

Comparison of Thermophilic–Mesophilic and Mesophilic–Thermophilic Two-Phase High-Solid Sludge Anaerobic Digestion at Different Inoculation Proportions: Digestion Performance and Microbial Diversity

Microbial electromethanogenesis for co2 valorisation and electrical energy storage

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