Molecular characterization of LvAV in response to white spot syndrome virus infection in the Pacific white shrimp (Litopenaeus vannamei)

He, Shulin; Song, Lei; Qian, Zhaoying; Hou, Fujun; Liu, Yongjie; Wang, Xian-Zong; Peng, Zhangming; Sun, Chengbo; Liu, Xiaolin

doi:10.1016/j.dci.2015.02.020

Cited by 18 publications

(3 citation statements)

References 29 publications

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“…However, some shrimp C-type lectins had apparently opposite functions during viral infection. It had been found that WSSV and yellow head virus (YHV) can hijack the shrimp C-type lectin for successful adhesion and entry into the host cell (Junkunlo et al, 2012;Wang et al, 2014;He et al, 2015). These different research results reflect the functional diversity and complexity of shrimp C-type lectins.…”

Section: Discussionmentioning

confidence: 99%

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

Zhao

Chen

et al. 2021

Front. Microbiol.

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As a new type of shrimp lethal virus, decapod iridescent virus 1 (DIV1) has caused huge economic losses to shrimp farmers in China. Up to now, DIV1 has been detected in a variety of shrimps, but there is no report in Marsupenaeus japonicus. In the current study, we calculated the LC50 to evaluate the toxicity of DIV1 to M. japonicus and determined through nested PCR that M. japonicus can be the host of DIV1. Through enzyme activity study, it was found that DIV1 can inhibit the activities of superoxide dismutase, catalase, lysozyme, and phenoloxidase, which could be a way for DIV1 to achieve immune evasion. In a comprehensive study on the transcriptomic changes of M. japonicus in response to DIV1 infection, a total of 52,287 unigenes were de novo assembled, and 20,342 SSR markers associated with these unigenes were obtained. Through a comparative transcriptomic analysis, 6,900 differentially expressed genes were identified, including 3,882 upregulated genes and 3,018 downregulated genes. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that some GO terms related to virus invasion, replication, and host antiviral infection were promoted under DIV1 infection, such as carbohydrate binding, chitin binding, chitin metabolic process, and DNA replication initiation, and some KEGG pathways related to immune response were significantly influenced by DIV1 infection, including Toll and IMD signaling pathway, JAK-STAT signaling pathway, IL-17 signaling pathway, C-type lectin receptor signaling pathway, complement and coagulation cascades, antigen processing and presentation, necroptosis, apoptosis, NOD-like receptor signaling pathway, apoptosis—multiple species, and TNF signaling pathway. Further analysis showed that STAT, Dorsal, Relish, heat shock protein 70 (HSP70), C-type lectins, and caspase play an important role in DIV1 infection. This is the first detailed study of DIV1 infection in M. japonicus, which initially reveals the molecular mechanism of DIV1 infection in M. japonicus by using the transcriptome analysis of hemocytes combined with enzyme activity study.

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Section: Discussionmentioning

confidence: 99%

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

Zhao

Chen

et al. 2021

Front. Microbiol.

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“…Differential expression of 19 proteins (32% of proteomic changes) concerned inflammation and immunity. Lectins are critical immune effectors of cellular defense in hemocytes of marine invertebrates (He et al, 2015;Watanabe et al, 2009) and we showed that two transmembrane lectin receptors (K1RFT9, K1PWN9) were down-regulated. The changes observed in the expression of the mannose receptor 1 (K1PWN9), a membrane receptor of immune cells involved in pathogen recognition (Jia et al, 2021), were indicative of changes in immunity.…”

Section: Boost Of Inflammation and Immunity In Intertidal Oystersmentioning

confidence: 89%

Harsh intertidal environment enhances metabolism and immunity in oyster (Crassostrea gigas) spat

Corporeau

Petton

Vilaça

et al. 2022

Marine Environmental Research

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“…In M. japonicus , a soluble stomach virus-associated CTL (MjsvCL) can capture and present virions to the cell-surface receptor to facilitate WSSV infection [14] . A C-type lectin (LvAV) from L. vannamei may be subverted by WSSV to enhance viral replication or immune avoidance [15] .…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of LvAV in response to white spot syndrome virus infection in the Pacific white shrimp (Litopenaeus vannamei)

Cited by 18 publications

References 29 publications

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

The Molecular Mechanism of Hemocyte Immune Response in Marsupenaeus japonicus Infected With Decapod Iridescent Virus 1

Harsh intertidal environment enhances metabolism and immunity in oyster (Crassostrea gigas) spat

Characterization and functional analysis of tandem threonine containing C-type lectin (Thr-Lec) from the ridgetail white prawn Exopalaemon carinicauda

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