Molecular Characterization of Listeria Monocytogenes Isolated from Different Animal-Origin Food Items from Urban and Rural Areas

Abstract

| Listeriosis is classified as the third main cause of food-borne diseases and it is one of the significant bacterial zoonotic infections causing high fatality rates worldwide. The aim of this research was to isolate and characterize Listeria monocytogenes (L. monocytogenes) from different food items in Sharkia Province, Egypt. A total of 200 food samples were collected aseptically from local markets and subjected to the phenotypic and genotypic characterization of L. monocytogenes. Listeria species were isolated from 28 (56%), 9 (18%), 3 (6%) and 5 (10%) of the minced meat, poultry meat, tilapia fish, and raw milk samples, respectively. Overall, L. monocytogenes were isolated from 12 and 8 samples from urban and rural areas with percentages of 6 and 4%, respectively. Of interest, inlA, actA, prfA, and hlyA virulence genes were detected in all 20 L. monocytogenes isolates by multiplex PCR. In conclusion, L. monocytogenes is an organism of public health implications, and its recovery from the food samples sold at retail outlets indicates a breach of quality assurance.

Molecular Detection of Listeria Species Isolated From Raw Milk with Special Reference to Virulence Determinants and Antimicrobial Resistance in Listeria monocytogenes

“…The presence of the hlyA gene in all strains examined for our study's virulotyping analysis was consistent with research by Osman et al (2016), Şanlıbaba et al (2018), Owusu-Kwarteng et al (2018), El-Demerdash et al (2019 and Skowron et al (2019). Additionally, it was found that L. monocytogenes' ability to form biofilms is impacted by the presence of the hlyA virulence gene (Price et al, 2018), as this organism is better able to withstand ineffective disinfection during cleaning procedures, which results in the continued presence of L. monocytogenes in the final product.…”

Progress in research on the safety of silicone rubber products in food processing

“…Elskens et al. (2012) proposed a general modeling framework based on the first and second migration values to predict the third migration results of SR samples. The main factors influencing the migration of cyclic siloxane oligomers from SR to food simulants are temperature, time, and the type of food simulant (i.e., n ‐hexane, n ‐heptane, and ethanol).…”

“…Listeriosis is the third leading cause of foodborne disease and one of the major zoonotic bacterial infections that cause high mortality worldwide. Listeria monocytogenes is the most common cause of listeriosis in humans and animals, owing to food and environmental pollution; it has a low incidence but a high case fatality rate (El‐Demerdash & Raslan, 2019; Sołtysiuk et al., 2022). L. monocytogenes has the ability to adhere to FCMs (Casarin et al., 2016; Gram et al., 2007; Iacumin et al., 2016).…”

“…Silicone rubber (SR) is an elastomer (rubberlike material) with an alternating backbone of silicon (Si) and oxygen (O) atoms, each with one or more hydrocarbon groups such as methyl, ethyl, or benzyl (Elskens et al., 2012; Wang et al., 2013). SR has the glass transition temperature ( T g ) of −127°C and is the most entropically elastic material of any entropy elastic material currently available (Halpin, 2014; Shit & Shah, 2013).…”

Isolation, phenotypic characterization and public health implications of Listeria monocytogenes circulating in smallholder dairy farms of Kombolcha town and Kutaber district, Amhara regional state, Ethiopia

“…Listeriosis is the main food-borne zoonotic illness because 99% of human infections are resulted from eating food products contaminated with mainly L. monocytogenes [2]. It is a Gram-positive, rod-shaped, motile, and non-spore-forming bacterium [3] and is primarily known as a veterinary pathogen, which causes basilar meningitis (circling disease) and abortion in sheep and cattle [4].…”