Molecular characterization of Leishmania spp. isolated from Brazilian stray dogs from an endemic area for canine visceral leishmaniasis

Coiro, C. J.; Coelho, Laiza Gabriela Gavioli; Silva, Rodrigo da; Langoni, Hélio

doi:10.1016/j.vprsr.2016.11.005

Cited by 2 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Leishmaniasis is endemic in South America, and epidemiological data are available from some of its countries: for example in Brazil, canine VL is caused mainly by L. infantum, which was detected in blood (28.05%), lymph nodes (57.32%), and bone marrow (69.51%) samples from stray dogs in a VL-endemic area (12). L. infantum (L. chagasi) is also responsible for leishmaniasis in domestic cats in Brazil (32).…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence, clinical, and pathological characteristics of canine leishmaniasis in a central region of Colombia

Picón

Almario

Rodríguez

et al. 2020

Journal of Veterinary Research

View full text Add to dashboard Cite

IntroductionLeishmaniasis is a zoonotic disease which is caused by protozoan parasites of the genus Leishmania. Canids are the most important reservoir of the parasites; however, limited data are available on the species of Leishmania prevalent in these animals and their impact on human health. The objective of this study was to estimate the seroprevalence of leishmaniasis in dogs from an inter-Andean region of Colombia during July 2016–July 2017, and to describe the clinical and histopathological features of the disease.Material and MethodsA total of 155 dogs were subjected to clinical examination and a serological test for detection of antibodies against Leishmania. Necropsy was carried out on positive animals and tissue samples were processed by routine histopathology.ResultsAltogether 19 dogs were positive in the serological test, establishing a 12% seroprevalence of Leishmania. Clinical examination and necropsy revealed exfoliative and ulcerative dermatitis with haemorrhagic borders on the ears, head, nose, and legs. Histopathology revealed severe multifocal dermatitis with abundant Leishmania amastigotes within the cytoplasm of phagocytic cells, depletion of lymphocytes in lymphoid tissues, interstitial pneumonia, and interstitial nephritis. Tissue samples were positive for Leishmania by PCR.ConclusionThe macro- and microscopic changes correlated with the presence of Leishmania as established by serological test and PCR.

show abstract

Section: Discussionmentioning

confidence: 99%

Seroprevalence, clinical, and pathological characteristics of canine leishmaniasis in a central region of Colombia

Picón

Almario

Rodríguez

et al. 2020

Journal of Veterinary Research

View full text Add to dashboard Cite

show abstract

“…Regarding the biological material analyzed, the choice of leukocyte pellet was based on the results of Mary et al (2004). They associated the sensitivity of molecular tests to the DNA extraction method and identified greater effectiveness of the tests when the nucleated cells were isolated from whole blood before digestion with Proteinase K. Although blood may not be the best option for detection of DNA by PCR (Lombardo et al 2012, Aschar et al 2016, Coiro et al 2017, the collection of this material is less invasive than bone marrow/lymph node. Furthermore, it facilitates the process, since optimizing collection time in canine epidemiological surveys has better acceptance and collaboration by the owners.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of visceral leishmaniasis in dogs from Barão de Melgaço, Pantanal region of Mato Grosso, Brazil

et al. 2021

View full text Add to dashboard Cite

The increasing expansion of visceral leishmaniasis (VL) in the Brazilian territory evidences the need for studies focused on the main reservoir of this parasite: the dog. This study aimed to conduct an epidemiological survey in the municipality of Barão de Melgaço, Pantanal region of the state of Mato Grosso (MT), Brazil. Conventional polymerase chain reaction (PCR) and qualitative SYBR®Green real-time PCR (qPCR) were used to diagnose canine VL (CVL) and characterize the factors associated with this infection. Of the 402 dogs that had blood samples collected, 31 presented the parasite DNA, representing a prevalence of 7.71% in the population studied. Positivity indices for PCR and qPCR were 3.48 (14/402) and 7.21% (29/402), respectively. Comparison of the results obtained by both techniques showed moderate agreement (Kappa = 0.5364). Of the independent variables analyzed, presence of clinical signs (p≤0.05) was the only one associated with CVL. Based on this study, we conclude that VL is a circulating disease, with relatively low prevalence, in dogs of Barão de Melgaço/MT, and that the presence of clinical signs is the only variable associated with canine infection.

show abstract

Molecular characterization of Leishmania spp. isolated from Brazilian stray dogs from an endemic area for canine visceral leishmaniasis

Cited by 2 publications

References 26 publications

Seroprevalence, clinical, and pathological characteristics of canine leishmaniasis in a central region of Colombia

Seroprevalence, clinical, and pathological characteristics of canine leishmaniasis in a central region of Colombia

Molecular detection of visceral leishmaniasis in dogs from Barão de Melgaço, Pantanal region of Mato Grosso, Brazil

Contact Info

Product

Resources

About