Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan

Kleschenko, Yuliya; Grybchuk, Danyil; Matveeva, Nadezhda S.; Macedo, Diego H.; Ponirovsky, E. N.; Lukashev, Alexander N.; Yurchenko, Vyacheslav

doi:10.3390/genes10100830

Cited by 23 publications

(27 citation statements)

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“…Symbiotic leishmaniaviruses have been associated with enhanced host type I interferon responses and contribute to the metastatic potential in L. Viannia species 58 . Although Leishmaniavirus has been detected at lower frequency in Old World L. major strains 59,60 , there is no conclusive data to suggest an involvement in pathogenesis and treatment failure. In Iranian cases, L. major infection was not influenced by the presence or absence of LRV2 61 .…”

Section: Discussionmentioning

confidence: 98%

Characterization of a new Leishmania major strain for use in a controlled human infection model

et al. 2021

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Leishmaniasis is widely regarded as a vaccine-preventable disease, but the costs required to reach pivotal Phase 3 studies and uncertainty about which candidate vaccines should be progressed into human studies significantly limits progress in vaccine development for this neglected tropical disease. Controlled human infection models (CHIMs) provide a pathway for accelerating vaccine development and to more fully understand disease pathogenesis and correlates of protection. Here, we describe the isolation, characterization and GMP manufacture of a new clinical strain of Leishmania major. Two fresh strains of L. major from Israel were initially compared by genome sequencing, in vivo infectivity and drug sensitivity in mice, and development and transmission competence in sand flies, allowing one to be selected for GMP production. This study addresses a major roadblock in the development of vaccines for leishmaniasis, providing a key resource for CHIM studies of sand fly transmitted cutaneous leishmaniasis.

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Section: Discussionmentioning

confidence: 98%

Characterization of a new Leishmania major strain for use in a controlled human infection model

et al. 2021

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“…Its presence is linked to the increased metastatic potential, parasite burden, immune response in mouse models of leishmaniasis and frequent treatment failures [ 434 , 435 ]. Notably, Old World leishmanias L. ( Leishmania ) possess a phylogenetically related Leishmaniavirus , LRV2, which is widespread in isolates of L. major [ 436 , 437 ], but its role in the disease progression is unknown. The phylogenies of viruses and their respective hosts are mainly congruent, suggesting long-term coevolution [ 438 ].…”

Section: Viruses In Euglenozoamentioning

confidence: 99%

Euglenozoa: taxonomy, diversity and ecology, symbioses and viruses

et al. 2021

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Euglenozoa is a species-rich group of protists, which have extremely diverse lifestyles and a range of features that distinguish them from other eukaryotes. They are composed of free-living and parasitic kinetoplastids, mostly free-living diplonemids, heterotrophic and photosynthetic euglenids, as well as deep-sea symbiontids. Although they form a well-supported monophyletic group, these morphologically rather distinct groups are almost never treated together in a comparative manner, as attempted here. We present an updated taxonomy, complemented by photos of representative species, with notes on diversity, distribution and biology of euglenozoans. For kinetoplastids, we propose a significantly modified taxonomy that reflects the latest findings. Finally, we summarize what is known about viruses infecting euglenozoans, as well as their relationships with ecto- and endosymbiotic bacteria.

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“…Symbiotic leishmaniaviruses have been associated with enhanced host type I interferon responses and contribute to the metastatic potential in L. Viannia species 54 . Although Leishmaniavirus has been detected at lower frequency in Old World L. major strains 55,56 , there is no conclusive data to suggest an involvement in pathogenesis and treatment failure. In Iranian cases, L. major infection was not influenced by the presence or absence of LRV2 57 .…”

Section: Discussionmentioning

confidence: 98%

Characterization of a newLeishmania majorisolate for use in a controlled human infection model

Ashwin

Sádlová

Vojtková

et al. 2020

Preprint

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Leishmaniasis is widely regarded as a vaccine-preventable disease, but the costs required to reach pivotal Phase 3 studies and uncertainty about which candidate vaccines should be progressed into human studies significantly limits progress in vaccine development for this neglected tropical disease. Controlled human infection models (CHIM) provide a pathway for accelerating vaccine development and to more fully understand disease pathogenesis and correlates of protection. Here, we describe the isolation, characterization and GMP manufacture of a new clinical isolate of Leishmania major. Two fresh isolates of L. major from Israel were initially compared by genome sequencing, in vivo infectivity and drug sensitivity in mice, and development and transmission competence in sand flies, allowing one (L. major_MRC-02) to be selected for GMP production. This study addresses a major roadblock in the development of vaccines for leishmaniasis, providing a key resource for CHIM studies of sand fly transmitted cutaneous leishmaniasis.

show abstract

Molecular Characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan

Cited by 23 publications

References 50 publications

Characterization of a new Leishmania major strain for use in a controlled human infection model

Characterization of a new Leishmania major strain for use in a controlled human infection model

Euglenozoa: taxonomy, diversity and ecology, symbioses and viruses

Characterization of a newLeishmania majorisolate for use in a controlled human infection model

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