Molecular characterization of larval anisakid nematodes from marine fishes off the Moroccan and Mauritanian coasts

Farjallah, Sarra; Busi, Marina; Mahjoub, Mohamed Ould; Slimane, Badreddine Ben; Paggi, L.; Saïd, Khaled; D’Amelio, Stefano

doi:10.1016/j.parint.2008.05.002

Cited by 41 publications

(25 citation statements)

References 19 publications

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“…Previously, both Pontes et al (2005) and Sequeira et al (2010) have detected high anisakid species richness (seven species) in fish from Madeira. Studies conducted at similar latitudes (Moroccan and Mauritanian Atlantic coasts) also reported high diversity of anisakid species (Farjallah et al 2008;Kijewska et al 2009). The region that includes Madeira, the Canary Islands, and the North African Atlantic coast is located at the confluence of different biogeographic areas, namely the North Atlantic, the Central Atlantic, and the Mediterranean, a factor which greatly contributes to an increased biodiversity.…”

Section: Discussionmentioning

confidence: 85%

Infection levels and diversity of anisakid nematodes in blackspot seabream, Pagellus bogaraveo, from Portuguese waters

et al. 2011

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The blackspot seabream, Pagellus bogaraveo, is a sparid fish of great economic importance in the northeast Atlantic. The main aim of this work was to assess the infection levels and diversity of anisakid nematodes parasitizing P. bogaraveo from Portuguese waters. The anisakid larvae were identified by polymerase chain reaction-restriction fragment length polymorphism analysis and ten different patterns were observed, four of which were not previously reported in the literature. Moreover, several species were detected for the first time in this host: Anisakis simplex × Anisakis pegreffii hybrids, Anisakis ziphidarum, Anisakis typica, Anisakis physeteris, as well as three undescribed anisakids Anisakis sp. PB-2009, Anisakis sp. PB-2010, and Contracaecum sp. PB-2010. The ITS1-5.8S-ITS2 region was sequenced and analyzed phylogenetically, revealing that our anisakids were distributed by the two distinct clades reported previously, corresponding to the two recognized larval morphotypes. Moreover, a group of organisms, including our specimens from Madeira and the previously reported Anisakis sp. HC-2005, cluster together and seem to belong to clade I. A certain degree of intraspecific diversity was also detected. Samples from mainland waters had the highest infection levels and were dominated by A. pegreffii. Madeira had the highest diversity overall, dominated by Anisakis sp. PB-2010. Fish from the Azores had the lowest infection levels, and the species with the highest relative abundance was A. physeteris. The anisakid nematode communities were relatively similar in mainland waters but very distinct in both the Azores and Madeira islands, suggesting the existence of at least three different stocks of P. bogaraveo in the northeast Atlantic.

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Section: Discussionmentioning

confidence: 85%

Infection levels and diversity of anisakid nematodes in blackspot seabream, Pagellus bogaraveo, from Portuguese waters

et al. 2011

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“…In the most marine fish, there is a wide range of common parasites, such as larvae and adults of A. simplex [17][18][19]. The A. simplex appear in fish at the larval stage and its infection of T. trachurus was previously reported in the Mediterranean and North Atlantic coasts [15,20,21].…”

Section: Discussionmentioning

confidence: 99%

Anisakis simplex (Nematoda: Anisakidae) from horse mackerel (Trachurus trachurus) in Atlantic coast of Morocco

Shawket¹

2017

APJTD

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“…It has indeed been demonstrated that mt markers based on the cox2 gene are useful for distinguishing sibling species of anisakids within Anisakis (see Valentini et al, 2006) and Contracaecum (both those maturing in pinnipeds-see Mattiucci et al, 2008a-and those from piscivorous birds-see Mattiucci et al, 2008b). On the other hand, the mtDNA cox2 also has been used by others to identify taxa belonging to Anisakis (see Umehara et al, 2007;Iglesias et al, 2008;Quiazon et al, 2008;Farjallah et al, 2008a, b). This finding seems to suggest that mitochondrial gene barcodes using cox2 sequences represent an effective identification method for sister-species as well as for detecting putative new species of anisakid nematodes.…”

Section: Discussionmentioning

confidence: 99%

“…The systematics of the species of Anisakis Dujardin, 1845 has been advanced in recent decades by the application of genetic molecular methodologies (Nascetti et al, 1986;Mattiucci et al, 1997Mattiucci et al, , 1998Mattiucci et al, , 2001Mattiucci et al, , 2002Mattiucci et al, , 2005D'Amelio et al, 2000;Nadler et al, 2005;Pontes et al, 2005;Valentini et al, 2006;Mattiucci & Nascetti, 2006Umehara et al, 2007Umehara et al, , 2008Farjallah et al, 2008a, b;Quiazon et al, 2008). The taxonomy of the species so far recognised in this genus has recently been reviewed (Mattiucci & Nascetti, 2008).…”

Section: Introductionmentioning

confidence: 99%

Anisakis nascettii n. sp. (Nematoda: Anisakidae) from beaked whales of the southern hemisphere: morphological description, genetic relationships between congeners and ecological data

2009

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A new anisakid nematode, Anisakis nascettii n. sp., is described from beaked whales Mesoplodon spp. off the coast of New Zealand and South Africa. Morphological and molecular (allozymes and mtDNA cox2 sequence) data were used for diagnostic and identification purposes. Among the 19 allozymes studied, 10 were found to be unique and characteristic for A. nascettii n. sp. Analysis of allozymes demonstrated reproductive isolation from A. ziphidarum Paggi, Nascetti, Webb, Mattiucci, Cianchi & Bullini, 1998 and mtDNA cox2 sequences depict this Anisakis species as a distinct and unique entity. Key morphological diagnostic traits for A. nascettii with respect to the genetically closely related species A. ziphidarum include: spicule length, the spicule/body length ratio, the arrangement of the caudal papillae and the shape of the plectanes of the adult males. Genetic data confirmed that Anisakis sp. A of Pontes et al. (2005), which was partly described by Iglesias et al. (2008), and Anisakis sp. of Valentini et al. (2006) are conspecific with A. nascettii. Both molecular and morphological data indicate that the new species belongs to the 'ziphidarum-group'; however, it is genetically very distinct from A. ziphidarum (D ( Nei ) = 0.69, K2P = 0.09), as well as from all of the previously genetically characterised Anisakis spp. All tree topologies inferred by different methods (MP, NJ and Bayesian) support the finding that A. nascettii n. sp. and A. ziphidarum are sister-species. It is also confirmed that A. nascettii n. sp. is, at the adult stage, a parasite of beaked whales of the genus Mesoplodon, whereas, as a larva, it has been identified from the squid Moroteuthis ingens Smith. Furthermore, Mesoplodon bowdoini Andrews represents a new host record for A. ziphidarum. The parallelism between the clade formed by these two anisakine taxa, i.e. A. ziphidarum and A. nascettii, and that formed by their definitive hosts further supports the hypothesis of host-parasite co-evolutionary relationships, as previously suggested for Anisakis spp. and their cetacean hosts.

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Molecular characterization of larval anisakid nematodes from marine fishes off the Moroccan and Mauritanian coasts

Cited by 41 publications

References 19 publications

Infection levels and diversity of anisakid nematodes in blackspot seabream, Pagellus bogaraveo, from Portuguese waters

Infection levels and diversity of anisakid nematodes in blackspot seabream, Pagellus bogaraveo, from Portuguese waters

Anisakis simplex (Nematoda: Anisakidae) from horse mackerel (Trachurus trachurus) in Atlantic coast of Morocco

Anisakis nascettii n. sp. (Nematoda: Anisakidae) from beaked whales of the southern hemisphere: morphological description, genetic relationships between congeners and ecological data

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