Molecular characterization of kisspeptin gene and effect of nano–encapsulted kisspeptin-10 on reproductive maturation in Catla catla

Rather, Mohd Ashraf; Bhat, Irfan Ahmad; Gireesh‐Babu, P.; Chaudhari, Aparna; Sundaray, Jitendra Kumar; Sharma, Rupam

doi:10.1016/j.domaniend.2016.01.005

Cited by 46 publications

(25 citation statements)

References 42 publications

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“…In other teleosts presenting two Kiss genes, both Kiss1 and Kiss2 mRNAs are also expressed in the central nervous system [RT-PCR and qPCR, zebrafish ( 40 – 42 ); RT-PCR, goldfish ( 43 ); RT-PCR, sea bass ( Dicentrarchus labrax ), medaka ( Oryzias latipes ) ( 42 ); RT-PCR and qPCR, chub mackerel ( 44 ); qPCR, rohu, Labeo rohita ( 45 ); qPCR, Catla catla ( 46 , 47 ); qPCR, pejerrey, Odontesthes bonariensis ( 48 )], suggesting that kisspeptins exert important functions in the teleost brain. Anatomical studies, using ISH and laser-capture microdissection coupled with qPCR, have demonstrated that each transcript is located in different brain nuclei: Kiss1 mRNA is observed in the ventral habenula , while Kiss2 mRNA is found in the preoptic region and in the hypothalamus [for review see Ref.…”

Section: Discussionmentioning

confidence: 99%

“…In other teleosts possessing two kisspeptin genes, different situations have been reported. In some species, both kisspeptins are detected in the gonads [medaka and zebrafish ( 42 , 53 ); sea bass ( 60 ); rohu ( 45 ); and Catla catla ( 46 , 47 )]. In contrast, in the chub mackerel, Kiss1 is expressed in the gonads of both sexes, while Kiss2 expression is not detected ( 44 ), whereas in the pejerrey, expression of both genes is very low in the gonads ( 48 ).…”

Section: Discussionmentioning

confidence: 99%

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Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression

et al. 2018

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The European eel (Anguilla anguilla) presents a blockade of sexual maturation at a prepubertal stage due to a deficient production of gonadotropins. We previously initiated, in the eel, the investigation of the kisspeptin system, one of the major gatekeepers of puberty in mammals, and we predicted the sequence of two Kiss genes. In the present study, we cloned and sequenced Kiss1 and Kiss2 cDNAs from the eel brain. The tissue distributions of Kiss1 and Kiss2 transcripts, as investigated by quantitative real-time PCR, showed that both genes are primarily expressed in the eel brain and pituitary. The two 10-residue long sequences characteristic of kisspeptin, eel Kp1(10) and Kp2(10), as well as two longer sequences, predicted as mature peptides, eel Kp1(15) and Kp2(12), were synthesized and functionally analyzed. Using rat Kiss1 receptor-transfected Chinese hamster ovary cells, we found that the four synthesized eel peptides were able to induce [Ca 2+ ]i responses, indicating their ability to bind mammalian KissR-1 and to activate second messenger pathways. In primary culture of eel pituitary cells, all four peptides were able to specifically and dose-dependently inhibit lhβ expression, without any effect on fshβ, confirming our previous data with heterologous kisspeptins. Furthermore, in this eel in vitro system, all four peptides inhibited the expression of the type 2 GnRH receptor (gnrh-r2). Our data revealed a dual inhibitory effect of homologous kisspeptins on both pituitary lhβ and gnrh-r2 expression in the European eel.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression

et al. 2018

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“…Mean particle size of G-AgNPs was found to be 35.4 nm. In nanotechnology, nanoparticles are particles between 1 and 100 nanometres in size (Auffan et al, 2009, Rather, Bhat, Gireesh, Aparna, Jeetendra & Sharma 2016. The distribution of particle size for G-AgNPs nanoparticles is given in Fig.…”

Section: Physicochemical Characterization Of Nanoparticles By Dls Andmentioning

confidence: 99%

Synthesis and characterization ofAzadirachta indicaconstructed silver nanoparticles and their immunomodulatory activity in fish

et al. 2016

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Green synthesis of nanoparticles by using different biological agents has emerged as an alternative to overcome the toxic effect of chemically synthesized nanoparticles. Among various biological agents, plants are mostly preferred. This study describes an eco‐friendly and green synthesis of silver nanoparticles (G‐AgNPs) using Azadirachta indica (neem) as a reducing agent. UV–Vis spectral analysis proved the wavelength of sample to be 420 nm, approaching the surface resonance peak specific for G‐AgNPs. Dynamic light scattering (DLS) analysis showed the mean diameter of particles as 35.4 nm with zeta potential +34.6 mV. TEM results revealed the compact and spherical shape of the particles. Fourier transform infrared spectroscopies (FT‐IR) demonstrate the presence of possible functional groups involved in synthesis of the silver nanoparticles. The functional activity of immunological parameters, such as nitroblue tetrazolium assay, myeloperoxidase activity, phagocytic activity, anti‐protease and lysozyme activity, increased significantly (P < 0.05) in fish treated with G‐AgNPs. Relative percentage survival (74%) and enhanced disease resistance were observed in G‐AgNP‐treated Cirrhinus mrigala fingerlings challenged with Aeromonas hydrophila. In summary, present results demonstrate biosynthesized silver nanoparticles have immunomodulatory and antibacterial activity.

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“…14 However, hsp70 showed a significant (p <0.05) up-regulation in the fish treated with 29nm Ag-15NPs at 400 µg l -1 on 1 st day and a slight increase to those exposed to 18nm at 100 µg l -1 as the 16 experiment progressed. In the mt gene, significant (p<0.05) up-regulation was observed in fish17 treated at 200 and 400 µg l -1 with 29nm size on 1 st and 7 th days, respectively.…”

mentioning

confidence: 94%

“…The liver tissues were digested using supra-16 pure (Merck, Germany) trace-metal free concentrated HNO3 and HCl in microwave assisted17 digestion system (Multiwave, Anton Parr, USA). After the digestion, the samples were dissolved18 in 5 ml of 0.1 M HNO3 and volume was made up to 10 ml using deionised water.…”

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confidence: 99%

Assessment of DNA damage and molecular responses in Labeo rohita (Hamilton, 1822) following short-term exposure to silver nanoparticles

Sharma

Rather

Ajima

et al. 2016

Food and Chemical Toxicology

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Molecular characterization of kisspeptin gene and effect of nano–encapsulted kisspeptin-10 on reproductive maturation in Catla catla

Cited by 46 publications

References 42 publications

Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression

Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression

Synthesis and characterization ofAzadirachta indicaconstructed silver nanoparticles and their immunomodulatory activity in fish

Assessment of DNA damage and molecular responses in Labeo rohita (Hamilton, 1822) following short-term exposure to silver nanoparticles

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