Molecular Characterization of Irish
            <i>Salmonella enterica</i>
            Serotype Typhimurium: Detection of Class I Integrons and Assessment of Genetic Relationships by DNA Amplification Fingerprinting

Daly, M.; Buckley, J. J. C.; Power, E.G.M.; O’Hare, Colette; Cormican, Martin; Cryan, B.; Fanning, Séamus

doi:10.1128/aem.66.4.1767-1767.2000

Cited by 19 publications

(41 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular characterisation of antimicrobial resistance in S. enterica isolates from Ireland and France also reported the presence of multiple integrons in strains obtained from human, animal and food sources [12,27]. These integrons preferentially carried one or two of the pse-1, aadA2, aadA1 and dhfrA1 gene cassettes confirming descriptions reported for the Spanish Salmonella.…”

Section: Integron Epidemiologysupporting

confidence: 75%

Importance of integrons in the diffusion of resistance

2001

View full text Add to dashboard Cite

Summary -Horizontal transfer of resistance genes is a successful mechanism for the transmission and dissemination of multiple drug resistance among bacterial pathogens. The impact of horizontally transmitted genetic determinants in the evolution of resistance is particularly evident when resistance genes are physically associated in clusters and transferred en bloc to the recipient cell. Recent advances in the molecular characterisation of antibiotic resistance mechanisms have highlighted the existence of genetic structures, called integrons, involved in the acquisition of resistance genes. These DNA elements have frequently been reported in multi-drug resistant strains isolated from animals and humans, and are located either on the bacterial chromosome or on broad-host-range plasmids. The role of integrons in the development of multiple resistance relies on their unique capacity to cluster and express drug resistance genes. Moreover, the spread of resistance genes among different replicons and their exchange between plasmid and bacterial chromosome are facilitated by the integration of integrons into transposable elements. The association of a highly efficient gene capture and expression system, together with the capacity for vertical and horizontal transmission of resistance genes represents a powerful weapon used by bacteria to combat the assault of antibiotics. antimicrobial resistance / integron / transposon / horizontal gene transferRésumé -Importance des intégrons dans la diffusion de la résistance. Le transfert horizontal de gènes est un mécanisme efficace pour la transmission et la dissémination, parmi les agents pathogènes bactériens, de résistances multiples aux antibiotiques. L'impact des déterminants génétiques transmis horizontalement dans l'évolution de la résistance est particulièrement évident lorsque les gènes de résistance sont physiquement regroupés, et transférés en bloc à la cellule réceptrice. Des avancées récentes dans la caractérisation moléculaire des mécanismes de résistance aux antibiotiques ont mis en évidence l'existence de structures génétiques, appelées intégrons, impliqués dans l'acquisition de gènes de résistance. Ces éléments d'ADN ont été fréquemment observés dans des souches bactériennes multi-résistantes isolées chez des animaux ou chez des humains, localisés soit sur le chromosome bactérien, soit sur des plasmides à large spectre d'hôte. Le rôle des intégrons dans le développement de la multi-résistance repose sur leur capacité unique à former des clusters de gènes et à exprimer des gènes Vet. Res. 32 (2001) 243-259 243

show abstract

Section: Integron Epidemiologysupporting

confidence: 75%

Importance of integrons in the diffusion of resistance

2001

View full text Add to dashboard Cite

show abstract

“…Class 1 and 2 integrons have been reported in S. sonnei (19). Class 1 integrons are frequently reported in clinical and veterinary isolates of the family Enterobacteriaceae and are associated with lateral transfer of antimicrobial resistance genes (5,10,13). The gene cassettes are integrated between two conserved segments at the 5Ј and 3Ј ends at a receptor attI1 site.…”

mentioning

confidence: 99%

Antimicrobial Resistance and Genetic Diversity of Shigella sonnei Isolates from Western Ireland, an Area of Low Incidence of Infection

et al. 2003

Self Cite

View full text Add to dashboard Cite

Shigella sonnei is a significant cause of gastroenteritis in both developing and industrialized countries. Definition of the diversity and antimicrobial susceptibility of S. sonnei isolates may be helpful in the management of individual cases and outbreaks. Antimicrobial susceptibility testing and pulsed-field gel electrophoresis (PFGE) were performed with 67 isolates of S. sonnei predominantly (n ‫؍‬ 59) from three counties in the west of Ireland. Phage typing (n ‫؍‬ 17), plasmid profiling (n ‫؍‬ 28), and integron analysis (n ‫؍‬ 24) were performed with subsets of strains. PFGE typing permitted recognition of two major clusters: PFGE type A (n ‫؍‬ 53) and PFGE type B (n ‫؍‬ 14). PFGE type A was associated with resistance to ampicillin, streptomycin, and sulfonamides (51 of 53 isolates), and those that were phage typed (n ‫؍‬ 6) were phage type 3. PFGE type B was associated with resistance to streptomycin, sulfonamides, tetracycline, and trimethoprim (11 of 14 isolates) and phage type 6 (9 of 11 isolates). Fifteen different plasmid profiles were identified among the 28 isolates analyzed. A class 2 integron was present in all 14 PFGE type B isolates. One of these isolates also contained a class 1 integron and showed a unique variant of the PFGE type B pattern. Sequence analysis of the gene cassette structures contained within these integrons identified distinct open reading frames that encoded determinants of resistance to trimethoprim, streptomycin, and streptothricin. Our data demonstrate two predominant PFGE types among S. sonnei isolates circulating in this region. The limited diversity of the S. sonnei isolates in this region means that detection of isolates indistinguishable by PFGE and according to their antibiograms in two or more patients is not persuasive evidence of a common-source food-or waterborne outbreak. Indistinguishable plasmid profiles in addition to indistinguishable PFGE and antibiogram types may be more suggestive of an epidemiologically relevant link between cases.

show abstract

“…By use of 37 different phages, serotype Typhimurium can be divided in more than 210 phage types (1). Besides serotyping and phage typing, powerful bacterial molecular typing methods, such as plasmid profiling, pulsed-field gel electrophoresis (PFGE), IS200 typing, ribotyping, random amplified polymorphic DNA analysis, and amplified fragment length polymorphism, are used for epidemiological investigation of salmonellae (3,7,8,13,16). These techniques are useful for defining clonal relationships between strains (17) and for assessing the distribution of Salmonella strains within food-processing environments (10,15).…”

mentioning

confidence: 99%

Phenotypic and Molecular Typing of Salmonella Strains Reveals Different Contamination Sources in Two Commercial Pig Slaughterhouses

Botteldoorn¹,

Herman²,

Rijpens³

et al. 2004

Appl Environ Microbiol

View full text Add to dashboard Cite

This study aimed to define the origin of Salmonella contamination on swine carcasses and the distribution of Salmonella serotypes in two commercial slaughterhouses during normal activity. Salmonellae were isolated from carcasses, from colons and mesenteric lymph nodes of individual pigs, and from the slaughterhouse environment. All strains were serotyped; Salmonella enterica serotype Typhimurium and Salmonella enterica serotype Derby isolates were additionally typed beyond the serotype level by pulsed-field gel electrophoresis (PFGE) and antibiotic resistance profiling (ARP); and a subset of 31 serotype Typhimurium strains were additionally phage typed. PFGE and ARP had the same discriminative possibility. Phage typing in combination with PFGE could give extra information for some strains. In one slaughterhouse, 21% of the carcasses were contaminated, reflecting a correlation with the delivery of infected pigs. Carcass contamination did not result only from infection of the corresponding pig; only 25% of the positive carcasses were contaminated with the same serotype or genotype found in the corresponding feces or mesenteric lymph nodes. In the other slaughterhouse, 70% of the carcasses were contaminated, and only in 4% was the same genotype or serotype detected as in the feces of the corresponding pigs. The other positive carcasses in both slaughterhouses were contaminated by genotypes present in the feces or lymph nodes of pigs slaughtered earlier that day or from dispersed sources in the environment. In slaughterhouses, complex contamination cycles may be present, resulting in the isolation of many different genotypes circulating in the environment due to the supply of positive animals and in the contamination of carcasses, probably through aerosols.

show abstract

Molecular Characterization of Irish Salmonella enterica Serotype Typhimurium: Detection of Class I Integrons and Assessment of Genetic Relationships by DNA Amplification Fingerprinting

Cited by 19 publications

References 0 publications

Importance of integrons in the diffusion of resistance

Importance of integrons in the diffusion of resistance

Antimicrobial Resistance and Genetic Diversity of Shigella sonnei Isolates from Western Ireland, an Area of Low Incidence of Infection

Phenotypic and Molecular Typing of Salmonella Strains Reveals Different Contamination Sources in Two Commercial Pig Slaughterhouses

Contact Info

Product

Resources

About