Molecular characterization of Irish E. coli O157:H7 isolates of human, bovine, ovine and porcine origin

Lenahan, M.; O’Brien, S.; Byrne, Catriona; Ryan, M. F.; Kennedy, C.A.; McNamara, Eleanor; Fanning, Séamus; Sheridan, J.J.; Sweeney, T.

doi:10.1111/j.1365-2672.2009.04320.x

Cited by 16 publications

(17 citation statements)

References 47 publications

(105 reference statements)

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“…The panel of virulence genes in this study included 69 targets, and our results were in agreement with those of another study suggesting that increasing the number of virulence genes in the panel would increase the resolution of the virulence gene profiling (57). Various virulence gene profiles in swine STEC strains have also been reported elsewhere (38)(39)(40)(41)(42)(58)(59)(60). However, it was challenging to compare the results of this study with the results of those previous reports because all of the studies employed different panels of virulence genes.…”

Section: Discussionsupporting

confidence: 81%

“…The degrees of genetic relatedness of swine STEC strains to strains from other animal species differed in those studies, and most studies focused on STEC O157:H7 (60,(69)(70)(71). Here, we found that STEC O59:H21 strains, which predominated in this swine population, were closely related among pigs in the same cohort.…”

Section: Discussionmentioning

confidence: 48%

“…Our study is the first one to use PFGE to analyze STEC strains recovered from repeated samples collected from pigs, while most of the previous studies used PFGE to determine the genetic relatedness of STEC strains from swine and other species (60,(69)(70)(71)(72). The degrees of genetic relatedness of swine STEC strains to strains from other animal species differed in those studies, and most studies focused on STEC O157:H7 (60,(69)(70)(71).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Diverse Virulence Gene Content of Shiga Toxin-Producing Escherichia coli from Finishing Swine

Tseng

Fratamico

Bagi

et al. 2014

Appl Environ Microbiol

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dShiga toxin-producing Escherichia coli (STEC) infections are a critical public health concern because they can cause severe clinical outcomes, such as hemolytic uremic syndrome, in humans. Determining the presence or absence of virulence genes is essential in assessing the potential pathogenicity of STEC strains. Currently, there is limited information about the virulence genes carried by swine STEC strains; therefore, this study was conducted to examine the presence and absence of 69 virulence genes in STEC strains recovered previously from finishing swine in a longitudinal study. A subset of STEC strains was analyzed by pulsed-field gel electrophoresis (PFGE) to examine their genetic relatedness. Swine STEC strains (n ‫؍‬ 150) were analyzed by the use of a high-throughput real-time PCR array system, which included 69 virulence gene targets. Three major pathotypes consisted of 16 different combinations of virulence gene profiles, and serotypes were determined in the swine STEC strains. The majority of the swine STEC strains (n ‫؍‬ 120) belonged to serotype O59:H21 and carried the same virulence gene profile, which consisted of 9 virulence genes: stx 2e , iha, ecs1763, lpfA O113 , estIa (STa), ehaA, paa, terE, and ureD. The eae, nleF, and nleH1-2 genes were detected in one swine STEC strain (O49:H21). Other genes encoding adhesins, including iha, were identified (n ‫؍‬ 149). The PFGE results demonstrated that swine STEC strains from pigs raised in the same finishing barn were closely related. Our results revealed diverse virulence gene contents among the members of the swine STEC population and enhance understanding of the dynamics of transmission of STEC strains among pigs housed in the same barn.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Diverse Virulence Gene Content of Shiga Toxin-Producing Escherichia coli from Finishing Swine

Tseng

Fratamico

Bagi

et al. 2014

Appl Environ Microbiol

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“…In a study carried out in Ireland, the prevalence of E. coli O157:H7 in pigs was found to be very low, and only four isolates were recovered from 1,710 pigs examined. However, three of these four contained the genes vtx2, eae, and hlyA, indicating their potential to cause illness in humans (68). While pork meat and products have generally not been implicated in human illness, in California in 2006, feral swine were implicated in contamination of agricultural fields and surface waterways with E. coli O157:H7, which caused a large outbreak linked to bagged spinach.…”

Section: Carriage Of Vtec In Pigsmentioning

confidence: 99%

Veterinary Public Health Approach to Managing Pathogenic Verocytotoxigenic Escherichia coli in the Agri-Food Chain

Duffy

McCabe

2014

Microbiol Spectr

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Verocytoxigenic Escherichia coli (VTEC) comprises many diverse serogroups, but seven serogroups, O157, O26, O103, O145, O111, O21, and O45, have been most commonly linked to severe human infections, though illness has also been reported from a range of other VTEC serogroups. This poses challenges in assessing the risk to humans from the diverse range of VTEC strains that may be recovered from animals, the environment, or food. For routine assessment of risk posed by VTEC recovered from the agri-food chain, the concept of seropathotype can be used to rank the human risk potential from a particular VTEC serogroup on the basis of both serotype (top seven serogroups) and the presence of particular virulence genes (vt in combination with eae, or aaiC plus aggR). But for other VTEC serogroups or virulence gene combinations, it is not currently possible to fully assess the risk posed. VTEC is shed in animal feces and can persist in the farm environment for extended periods ranging from several weeks to many months, posing an ongoing reservoir of contamination for grazing animals, water courses, and fresh produce and for people using farmland for recreational purposes. Appropriate handling and treatment of stored animal waste (slurries and manures) will reduce risk from VTEC in the farm environment. Foods of animal origin such as milk and dairy products and meat may be contaminated with VTEC during production and processing, and the pathogen may survive or grow during processing operations, highlighting the need for well-designed and validated Hazard Analysis Critical Control Point management systems. This article focuses on a veterinary public health approach to managing VTEC, highlighting the various routes in the agri-food chain for transmission of human pathogenic VTEC and general approaches to managing the risk.

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“…Comparative studies in Ireland, Sweden and Japan revealed that cattle isolates possessing same phenotypic and genotypic traits as human clinical isolates have potential for causing human disease (Lenahan et al, 2009;Aspan and Eriksson, 2010;Lee et al, 2011). Kim et al (1999) suggested that E. coli O157:H7 strains isolated from diseased humans were members of a different lineage than strains typically isolated from healthy cattle.…”

Section: Introductionmentioning

confidence: 99%

Bovine Shiga toxin producing Escherichia coli O157:H7 of Bangladesh: Is it capable of causing diseases similar to clinical strains?

Rabbi¹,

Yasmin²,

Nessa³

et al. 2014

Afr. J. Microbiol. Res.

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Escherichia coli O157:H7 is a predominant serotype of Shiga toxin producing E. coli (STEC) and is responsible for many outbreaks worldwide. Until recently, there is no comparative study on the bovine and clinical isolates as no STEC O157:H7 has been isolated from patients in Bangladesh. In the present study, the local bovine isolates were compared with a reference clinical strain to investigate whether the bovine isolates are capable of producing same degree of illness as the clinical strain. Two local isolates (CD-11 and CD-17) of bovine origin and a reference clinical strain (E. coli O157:H7 NCTC 12079) were investigated for the presence of virulence genes by polymerase chain reaction (PCR), Shiga toxin production by VTEC-RPLA, invasive property by Congo red binding and Sereny's keratoconjunctivitis, and other enterotoxic, cytotoxic and mouse lethal activities. Both the local isolates and the reference clinical strain showed the presence of eae and stx 2 genes and were found to be non-invasive. The isolates also produced enterotoxin, cytotoxin and mouse lethality similar to that found with the reference strain. All these results strongly suggest that the local bovine STEC O157:H7 isolates have potential to cause diseases similar to clinical STEC O157:H7 strain, which might lead to any outbreak in Bangladesh.

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Molecular characterization of Irish E. coli O157:H7 isolates of human, bovine, ovine and porcine origin

Cited by 16 publications

References 47 publications

Diverse Virulence Gene Content of Shiga Toxin-Producing Escherichia coli from Finishing Swine

Diverse Virulence Gene Content of Shiga Toxin-Producing Escherichia coli from Finishing Swine

Veterinary Public Health Approach to Managing Pathogenic Verocytotoxigenic Escherichia coli in the Agri-Food Chain

Bovine Shiga toxin producing Escherichia coli O157:H7 of Bangladesh: Is it capable of causing diseases similar to clinical strains?

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