Molecular Characterization of Integron Class 1 (In196) Encoding the VIM-2 Metallo-β-Lactamase of<i>pseudomonas aeruginosa</i>Isolated from a Hospital Environment

Garza–Ramos, Ulises; Tinoco, Perla; Rojas, Thaís Cabrera Galvão; Carrillo, Berta; Barajas, Juan Manuel; Suárez, Sandra; Silva-Sánchez, Jesús

doi:10.1179/joc.2009.21.5.590

Cited by 9 publications

(3 citation statements)

References 3 publications

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“…One isolate (5104), bla GES-1 , was found in a new class 1 integron (genes GES-1-tniC) that lack the 3′CS but has the tniC (transposase), named In173 according to INTEGRALL data base. The MβL VIM-2-containing In559 (aacA7, VIM-2, dhfrB5, aacC5, tniC) class 1 integron identified in this work (isolate 6102) was previously described in a nosocomial outbreak of P. aeruginosa in the United States (Lolans et al, 2005); In559 differs from the In196 (VIM-2, aacA4, aac (6′)-IIa, OXA-2) class 1 integron identified in the P. aeruginosa isolates previously described in Mexico (Garza-Ramos et al, 2009). In P. aeruginosa, the chromosomal or plasmid localization of class 1 integrons encoding the carbapenemase gene is crucial for its dissemination; however, its location is rarely studied (Stokes et al, 2012).…”

mentioning

confidence: 89%

Widespread of ESBL- and carbapenemase GES-type genes on carbapenem-resistant Pseudomonas aeruginosa clinical isolates: a multicenter study in Mexican hospitals

Garza–Ramos

Barrios

Reyna-Flores

et al. 2015

Diagnostic Microbiology and Infectious Disease

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mentioning

confidence: 89%

Widespread of ESBL- and carbapenemase GES-type genes on carbapenem-resistant Pseudomonas aeruginosa clinical isolates: a multicenter study in Mexican hospitals

Garza–Ramos

Barrios

Reyna-Flores

et al. 2015

Diagnostic Microbiology and Infectious Disease

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“…The P. aeruginosa isolates P1165, P1483, P1503, P1546, and P1547 were obtained from clinical samples in the Instituto Nacional de Rehabilitación (National Institute of Rehabilitation) Luis Guillermo Ibarra Ibarra in Mexico City and identified by a semiautomated system Vitek 2 Compact® (bioMereux Marcy l'Etoile, France). P. aeruginosa isolates P6103 and P3536 were obtained from clinical environments [ 40 , 41 ]. Clinical isolates were confirmed as P. aeruginosa by phylogenetic analysis of their 16S rRNA gene using the EZBioCloud database [ 43 ] ( Table 2 ).…”

Section: Methodsmentioning

confidence: 99%

Pseudomonas aeruginosa Isolates from Water Samples of the Gulf of Mexico Show Similar Virulence Properties but Different Antibiotic Susceptibility Profiles than Clinical Isolates

Romero-González,

Montelongo-Martínez,

González-Valdez

et al. 2024

International Journal of Microbiology

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Pseudomonas aeruginosa is an opportunistic pathogen found in a wide variety of environments, including soil, water, and habitats associated with animals, humans, and plants. From a One Health perspective, which recognizes the interconnectedness of human, animal, and environmental health, it is important to study the virulence characteristics and antibiotic susceptibility of environmental bacteria. In this study, we compared the virulence properties and the antibiotic resistance profiles of seven isolates collected from the Gulf of Mexico with those of seven clinical strains of P. aeruginosa. Our results indicate that the marine and clinical isolates tested exhibit similar virulence properties; they expressed different virulence factors and were able to kill Galleria mellonella larvae, an animal model commonly used to analyze the pathogenicity of many bacteria, including P. aeruginosa. In contrast, the clinical strains showed higher antibiotic resistance than the marine isolates. Consistently, the clinical strains exhibited a higher prevalence of class 1 integron, an indicator of anthropogenic impact, compared with the marine isolates. Thus, our results indicate that the P. aeruginosa marine strains analyzed in this study, isolated from the Gulf of Mexico, have similar virulence properties, but lower antibiotic resistance, than those from hospitals.

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“…On the other hand, the pan-susceptible strain, Escherichia coli ATCC 25922 was used as negative control. Previously described P. aeruginosa clinical strains harboring bla VIM , bla IMP , bla OXA-23 , bla OXA-40 , and bla OXA-48 were also used as positive controls; they were kindly provided by Dr. Garza-Ramos and Dr. Silva-Sánchez from the Instituto Nacional de Salud Pública in Cuernavaca, Morelos, Mexico [24][25][26][27].…”

Section: Genotypic Detection Of Carbapenemasesmentioning

confidence: 99%

Acquired blaVIM and blaGES Carbapenemase-Encoding Genes in Pseudomonas aeruginosa: A Seven-Year Survey Highlighting an Increasing Epidemiological Threat

Martínez-Zavaleta,

Fernández-Rodríguez,

Hernández-Durán

et al. 2023

Pathogens

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(1) Background: Pseudomonas aeruginosa is a Gram-negative bacterium with several intrinsic and acquired antimicrobial resistance mechanisms. The spread of carbapenemase-encoding genes, an acquired mechanism, enables carbapenem resistance in clinical settings. Detection of the carbapenemase-producer strains is urgent. Therefore, we aimed to characterize carbapenemase production in the clinical strains of P. aeruginosa at a tertiary-care center. (2) Methods: We included clinical strains of P. aeruginosa (from August 2011 to December 2018) with resistance towards at least one carbapenem. Strains were isolated in a tertiary-care center in Mexico City. Antimicrobial susceptibility profiles were determined by broth microdilution. Screening for carbapenemase-encoding genes was performed in all strains. Phenotypic assays (CarbaNP and mCIM) were conducted. Additional modifications to mCIM were also tested. (3) Results: One-hundred seventy-one P. aeruginosa strains out of 192 included in this study were resistant towards at least one of the carbapenems tested. Forty-seven of these strains harbored a carbapenemase-encoding gene. VIM (59.6%) and GES (23.4%) were the most frequently found carbapenemases in our study, followed by IMP (14.9%). (4) Among the most frequent carbapenemase genes identified, metallo-ß-lactamases were the most prevalent, which impair new treatment options. Searching for carbapenemase genes should be performed in resistant isolates to stop transmission and guide antimicrobial treatment.

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Molecular Characterization of Integron Class 1 (In196) Encoding the VIM-2 Metallo-β-Lactamase ofpseudomonas aeruginosaIsolated from a Hospital Environment

Cited by 9 publications

References 3 publications

Widespread of ESBL- and carbapenemase GES-type genes on carbapenem-resistant Pseudomonas aeruginosa clinical isolates: a multicenter study in Mexican hospitals

Widespread of ESBL- and carbapenemase GES-type genes on carbapenem-resistant Pseudomonas aeruginosa clinical isolates: a multicenter study in Mexican hospitals

Pseudomonas aeruginosa Isolates from Water Samples of the Gulf of Mexico Show Similar Virulence Properties but Different Antibiotic Susceptibility Profiles than Clinical Isolates

Acquired blaVIM and blaGES Carbapenemase-Encoding Genes in Pseudomonas aeruginosa: A Seven-Year Survey Highlighting an Increasing Epidemiological Threat

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