Molecular Characterization of<i>Leishmania</i>Parasites in Giemsa-Stained Slides from Cases of Human Cutaneous and Visceral Leishmaniasis, Eastern Algeria

Beldi, Nadia; Mansouri, Rachid; Bettaieb, Jihéne; Yaacoub, Alia; Souguir, Hejer; Aoun, Yusr Saadi Ben; Saadni, Farida; Guizani, Ikram; Guerbouj, Souheila

doi:10.1089/vbz.2016.2071

Cited by 8 publications

(4 citation statements)

References 34 publications

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“…In our study the highest number of specific positive results (highest sensitivity) were observed when PCR amplification was performed on DNA extracted from direct slides for both FTG and CL. These results show concordance with data published by other investigators[41,42].…”

Section: Discussionsupporting

confidence: 94%

Fish tank granuloma: An emerging skin disease in Iran mimicking Cutaneous Leishmaniasis

et al. 2019

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ObjectiveMycobacterium marinum causes a rare cutaneous disease known as fish tank granuloma (FTG). The disease manifestations resemble those associated with Cutaneous Leishmaniasis (CL). The aim of this study was to determine whether FTG was the cause of cutaneous lesions in patients who were referred to the Parasitology laboratory of Imam Reza Hospital in Mashhad to be investigated for CL.Materials/MethodsOne hundered patients, clinically diagnosed with CL between April 2014 and March 2015, were included in this study. Ziehl-Neelsen staining was performed to identify acid-fast Mycobacterium in addition to bacterial cultures using Löwenstein-Jensen medium. Skin lesion samples were also collected and kept on DNA banking cards for PCR testing.ResultsTwenty-nine of the 100 individuals with skin lesions, and therefore suspected of suffering from CL, tested positive for Mycobacterium marinum by PCR. Of these, 21 (72.4%) were male and 8(27.6%) were female. In 97% of these cases the lesions were located on hands and fingers. These patients had a history of manipulating fish and had been in contact with aquarium water. A sporotrichoid appearance was observed in 58.6% of the patients with mycobacterial lesions; 67% of patients had multiple head appearance.ConclusionPatients suspected to have CL and who test negative for CL could be affected by FTG. Therefore, after obtaining an accurate case history, molecular diagnosis is recommended for cases that give a negative result by conventional methods.

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Section: Discussionsupporting

confidence: 94%

Fish tank granuloma: An emerging skin disease in Iran mimicking Cutaneous Leishmaniasis

et al. 2019

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“…Optimal sampling, storage and transport to a receiving laboratory (such as the Centres for Disease Control and Prevention, US, or Fiocruz, Brazil) is critical. Before detection, DNA must first be extracted and purified; this is often performed by using commercial kits, such as the silica-based DNeasy Blood and Tissue Kit (Qiagen, Germany), NucliSENS easyMAG system (BioMerieux, France) or via in-house extraction protocols based on phenol extraction and ethanol precipitation [ 36 , 140 , 141 ]. Some rapid extraction methods have been developed recently, e.g., SpeedXtract (Qiagen, Hilden, Germany), which greatly reduce the time to test result [ 142 ].…”

Section: Methods For the Detection And Diagnosis Of Leishmaniasismentioning

confidence: 99%

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

et al. 2022

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Leishmania infections span a range of clinical syndromes and impact humans from many geographic foci, but primarily the world’s poorest regions. Transmitted by the bite of a female sand fly, Leishmania infections are increasing with human movement (due to international travel and war) as well as with shifts in vector habitat (due to climate change). Accurate diagnosis of the 20 or so species of Leishmania that infect humans can lead to the successful treatment of infections and, importantly, their prevention through modelling and intervention programs. A multitude of laboratory techniques for the detection of Leishmania have been developed over the past few decades, and although many have drawbacks, several of them show promise, particularly molecular methods like polymerase chain reaction. This review provides an overview of the methods available to diagnostic laboratories, from traditional techniques to the now-preferred molecular techniques, with an emphasis on polymerase chain reaction-based detection and typing methods. Graphical abstract

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“…The use of PCR to detect leishmanial DNA in Giemsa-stained smear scrapings can be used in investigations of the reservoir hosts and in research conducted with infected animal models 9 and therefore, this technique can be applied in Sri Lanka to identify the reservoir hosts. A previous study conducted by Beldi et al, (2017) 10 has confirmed the usefulness of slides for PCR identification of Leishmania parasites in retrospective epidemiological investigations. 10 Therefore, this method can be adopted for similar studies conducted in Sri Lanka.…”

Section: Limitations Of the Present Study And Future Directionsmentioning

confidence: 65%

“…A previous study conducted by Beldi et al, (2017) 10 has confirmed the usefulness of slides for PCR identification of Leishmania parasites in retrospective epidemiological investigations. 10 Therefore, this method can be adopted for similar studies conducted in Sri Lanka.…”

Section: Limitations Of the Present Study And Future Directionsmentioning

confidence: 65%

Genomic DNA extraction and amplification of Leishmania donovani using polymerase chain reaction (PCR) from archived, Giemsa- stained slides

Amarasinghe

Iddawela

2021

Sri Lankan J Infec Dis

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Molecular Characterization ofLeishmaniaParasites in Giemsa-Stained Slides from Cases of Human Cutaneous and Visceral Leishmaniasis, Eastern Algeria

Cited by 8 publications

References 34 publications

Fish tank granuloma: An emerging skin disease in Iran mimicking Cutaneous Leishmaniasis

Fish tank granuloma: An emerging skin disease in Iran mimicking Cutaneous Leishmaniasis

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

Genomic DNA extraction and amplification of Leishmania donovani using polymerase chain reaction (PCR) from archived, Giemsa- stained slides

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