Molecular characterization of<i>Chlamydia</i>species in commercial and backyard poultry farms in Costa Rica

Morales, Álvaro; Dolz, Gaby

doi:10.1017/s0950268821002715

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…However, few C. psittaci-specific surveillance and genotyping studies (and no genomes sequenced from the South Americas) exist to date [8]. Among the limited number of South American studies conducted, the majority only performed partial C. psittaci ompA characterisation [61][62][63][64][65], of which ompA genotype A was detected the most, followed by a lower frequency of genotypes B, E/B and WC. To contextualise these findings within a global phylogenetic framework and to estimate strain emergence, additional WGS of C. psittaci strains from South America is required, particularly from the clonal ST24 lineages and genetically distinct E/B and WC genotypes (in which little WGS data currently exists).…”

Section: What About C Psittaci Strains From South America?mentioning

confidence: 99%

Whole-genome sequencing of Chlamydia psittaci from Australasian avian hosts: A genomics approach to a pathogen that still ruffles feathers

et al. 2023

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Chlamydia psittaci is a globally distributed veterinary pathogen with zoonotic potential. Although C. psittaci infections have been reported in various hosts, isolation and culture of Chlamydia is challenging, hampering efforts to produce contemporary global C. psittaci genomes. This is particularly evident in the lack of avian C. psittaci genomes from Australia and New Zealand. In this study, we used culture-independent probe-based whole-genome sequencing to expand the global C. psittaci genome catalogue. Here, we provide new C. psittaci genomes from two pigeons, six psittacines, and novel hosts such as the Australian bustard (Ardeotis australis) and sooty shearwater (Ardenna grisea) from Australia and New Zealand. We also evaluated C. psittaci genetic diversity using multilocus sequence typing (MLST) and major outer membrane protein (ompA) genotyping on additional C. psittaci -positive samples from various captive avian hosts and field isolates from Australasia. We showed that the first C. psittaci genomes sequenced from New Zealand parrots and pigeons belong to the clonal sequence type (ST)24 and diverse ‘pigeon-type’ ST27 clade, respectively. Australian parrot-derived strains also clustered in the ST24 group, whereas the novel ST332 strain from the Australian bustard clustered in a genetically diverse clade of strains from a fulmar, parrot, and livestock. MLST and ompA genotyping revealed ST24/ompA genotype A in wild and captive parrots and a sooty shearwater, whilst ‘pigeon-types’ (ST27/35 and ompA genotypes B/E) were found in pigeons and other atypical hosts, such as captive parrots, a little blue penguin/Kororā (Eudyptula minor) and a zebra finch (Taeniopygia guttata castanotis) from Australia and New Zealand. This study provides new insights into the global phylogenomic diversity of C. psittaci and further demonstrates the multi-host generalist capacity of this pathogen.

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Section: What About C Psittaci Strains From South America?mentioning

confidence: 99%

Whole-genome sequencing of Chlamydia psittaci from Australasian avian hosts: A genomics approach to a pathogen that still ruffles feathers

et al. 2023

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“…Among these, C. psittaci , C. gallinacea , C. abortus , C. muridarum , C. suis , and C. pecorum are able to infect poultry. The first three species naturally infect poultry, whereas the latter three are only sporadically detected in birds, presumably after close contact of the birds with their respective natural hosts [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Belgian Cross-Sectional Epidemiological Study on Zoonotic Avian Chlamydia spp. in Chickens

De Meyst,

De Clercq,

Porrez

et al. 2024

Microorganisms

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Chlamydia psittaci, Chlamydia gallinacea, and Chlamydia abortus are the most common Chlamydia spp. in chickens and have a confirmed or suggested zoonotic potential. No recent data are available on their prevalence and impact in the Belgian chicken industry or in the recreational chicken branch. Therefore, a cross-sectional epidemiological study was executed where samples were collected from both factory-farmed and backyard chickens. More specifically, pharyngeal chicken swabs were obtained from 20 chicken farms, 5 chicken abattoirs, and 38 different backyard locations and were analyzed using species-specific Polymerase Chain Reactions (PCRs) for the presence of the three avian Chlamydia spp. To investigate their zoonotic potential, samples were simultaneously collected from 54 backyard chicken caretakes and 37 professional chicken caretakers or abattoir employees and analyzed using species-specific PCRs as well. This study confirmed the presence of DNA of all three Chlamydia species in both the chicken industry and backyard settings. Chlamydia psittaci was the most prevalent in the industry chickens (11.0%), whereas Chlamydia gallinacea was the dominant species in the backyard chickens (14.5%). Chlamydia abortus infections were more common in the commercial chickens (9.0%) compared to the backyard chickens (2.6%). The DNA of all three species was also detected in humans (3.9% Chlamydia psittaci, 2.9% Chlamydia gallinacea, and 1.0% Chlamydia abortus).

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“…Chlamydia psittaci infections in humans are well-documented [6][7][8][9][10][11][12][13][14][15]. Most reported human C. psittaci infection cases are attributed to close contact with poultry [16][17][18][19][20][21][22][23][24][25]. To date, the largest psittacosis outbreak was reported in the United States in August 2018, with 82 poultry processing plant workers diagnosed with C. psittaci infection [26].…”

Section: Introductionmentioning

confidence: 99%

Chlamydia psittaci detected at a live poultry wholesale market in central China

Zhang,

Fu,

Luo

et al. 2024

Preprint

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Background We investigated the presence of Chlamydia psittaci in poultry and the environment in live poultry wholesale markets in Changsha during 2021–2022 and conducted phylogenetic analysis to understand its distribution in this market. Methods In total, 483 samples were analyzed using real-time polymerase chain reaction and 17 C. psittaci-positive samples using high-throughput sequencing, BLAST similarity, and phylogenetic analysis. Results Twenty two out of 483 poultry and environmental samples were positive for C. psittaci (overall positivity rate: 4.55%) with no difference in positivity rates over 12 months. Chlamydia psittaci was detected at 11 sampling points (overall positivity rate: 27.5%), including chicken, duck, and pigeon/chicken/duck/goose shops, with pigeon shops having the highest positivity rate (46.67%). The highest positivity rates were found in sewage (12.5%), poultry fecal (7.43%), cage swab (6.59%), avian pharyngeal/anorectal swab (3.33%), and air (2.29%) samples. The ompA sequences were identified in two strains of C. psittaci, which were determined to bear genotype B using phylogenetic analysis. Thus, C. psittaci genotype B was detected in the poultry and environmental samples from the poultry wholesale market in Changsha during monitoring. Conclusions Monitoring programs for C. psittaci in live markets need to be strengthened to address the possible zoonotic threat.

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Molecular characterization ofChlamydiaspecies in commercial and backyard poultry farms in Costa Rica

Cited by 6 publications

References 29 publications

Whole-genome sequencing of Chlamydia psittaci from Australasian avian hosts: A genomics approach to a pathogen that still ruffles feathers

Whole-genome sequencing of Chlamydia psittaci from Australasian avian hosts: A genomics approach to a pathogen that still ruffles feathers

Belgian Cross-Sectional Epidemiological Study on Zoonotic Avian Chlamydia spp. in Chickens

Chlamydia psittaci detected at a live poultry wholesale market in central China

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