Molecular characterization of human adenoviruses in urban wastewaters using next generation and Sanger sequencing

Iaconelli, Marcello; Valdazo-González, Begoña; Equestre, Michele; Ciccaglione, Anna Rita; Marcantonio, Cinzia; Libera, S. Della; Rosa, Giuseppina La

doi:10.1016/j.watres.2017.05.039

Cited by 53 publications

(33 citation statements)

References 47 publications

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“…The results of this study show that the presence of HBoV is a common and steady feature in the water environment where shellfish are grown and harvested and corroborate data from an environmental study conducted in 2016 in Italy, in which HBoVs were detected in 79% of urban wastewaters (30), with a predominance of species 2 and 3. Moreover, HBoV species 2 and 3 were found to be the most frequently detected viruses in river waters in Italy (31), outnumbering even adenovirus, known to be widespread in water environments (46). The high rate of detection of HBoV in FIG 1 Phylogenetic tree constructed with the partial HBoV VP1/2 sequences.…”

Section: Resultsmentioning

confidence: 99%

Detection of Human Bocavirus Species 2 and 3 in Bivalve Shellfish in Italy

Rosa

Purpari

Guercio

et al. 2018

Appl Environ Microbiol

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Human bocavirus (HBoV) has been shown to be a common cause of respiratory infections and gastroenteritis in children. Recently, HBoVs have been detected in sewage and river waters in Italy and worldwide. However, studies on their presence in other water environments and in bivalve mollusks are not yet available. In this study, 316 bivalve shellfish samples collected in three Italian regions over a 6-year period (2012 to 2017) were analyzed by nested PCR and sequencing using broad-range primer pairs targeting the capsid proteins VP1 and VP2 of HBoV. The virus was detected in 27 samples (8.5% of the total samples), and a statistically significant difference was found within the three regions. A further 13 samples, collected in geographic and temporal proximity to positive samples, were included in the study to assess the spread of HBoV in shellfish production areas at the time of contamination. Twelve of these additional samples were found to be positive for HBoV. All positive samples in this study were characterized as HBoV species 2 (17 samples; 8 different sequences) or species 3 (22 samples; 4 different sequences). This study reports the occurrence of HBoV in bivalve shellfish and shows evidence of considerable spatial spread of the virus throughout shellfish production areas. Further studies are needed to elucidate both the role of HBoV as an agent of gastroenteritis and the risk for foodborne transmission of this virus. Human bocavirus is recognized as an important cause of acute respiratory tract infections and has recently been considered an etiological agent of gastroenteritis in the pediatric population. Our findings document that HBoVs are detected in bivalve shellfish with a relevant prevalence and suggest that an assessment of the risk for foodborne transmission of these viruses should be undertaken.

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Section: Resultsmentioning

confidence: 99%

Detection of Human Bocavirus Species 2 and 3 in Bivalve Shellfish in Italy

Rosa

Purpari

Guercio

et al. 2018

Appl Environ Microbiol

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“…In this study, NGS was useful to detect, in positive samples, rare types undetected by Sanger sequencing concealed by prevalent genotypes, as shown in previous investigations (Iaconelli et al . ; Suffredini et al . ), confirming the usefulness of wastewater surveillance coupled with NGS to study the molecular epidemiology of enteric viruses.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of human Sapovirus in untreated sewage in Italy by amplicon-based Sanger and next-generation sequencing

et al. 2018

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Aims: Human Sapoviruses (HSaVs) are etiological agents of sporadic cases and outbreaks of acute gastroenteritis in humans of all ages. Evidence of worldwide distribution of HSaV has been documented; however, little is known about HSaV circulation in Italy. To study their occurrence and genetic diversity a nation-wide environmental surveillance was undertaken. Methods and Results: One hundred and sixty-six raw sewage samples, collected from 16 wastewater treatment plants throughout Italy, were processed and analysed by a RT-nested PCR targeting the capsid region, followed by amplicon sequencing. HSaV was detected in 56 of 166 (33Á7%) samples, characterized as genotypes GI.1 (n = 30 samples), GI.2 (n = 3), GI.3 (n = 2) and GII.

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“…To date, 79 HAdV types have been identified and are classified into seven species (A‐G) . HAdV types 1 to 7 account for approximately 80% of all HAdV‐infected cases in children .…”

Section: Introductionmentioning

confidence: 99%

Epidemiology of human adenovirus infection in children hospitalized with lower respiratory tract infections in Hunan, China

Xie

Zhang

Xiao

et al. 2018

Journal of Medical Virology

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To investigate the current genotypes of circulating human adenovirus (HAdV) strains, we molecularly genotyped HAdV in the nasopharyngeal aspirates (NPAs) of patients with acute lower respiratory tract infections (ALRTIs) and attempted to determine their associations with clinical symptoms. A total of 4751 NPA samples were collected from 4751 patients admitted to Hunan Provincial People's Hospital from September 2007 to March 2014, of which 447 (9.4%) samples were HAdV positive. Fourteen different HAdV types were identified; HAdV types 1 to 7 (HAdV 1‐7) were identified in 95.7% of the 447 NPA samples with HAdV‐7 and HAdV‐3 being the most prevalent. In addition, 93.3% (417 of 447) of patients were younger than 5 years. The incidence of HAdV infection peaked in summer. Different HAdV types showed a predilection for different age groups and different seasonal distribution patterns. Coinfection of HAdVs and other respiratory viruses was detected in 63.3% (283 of 447) of the HAdV‐positive samples. The most common clinical diagnosis was pneumonia and the most common symptoms were fever and cough. In comparison with children infected with HAdV‐3 alone, those infected with HAdV‐7 alone had an increased frequency of severe pneumonia involvement (11.6% vs 32.4%; P = 0.031), higher intensive care unit admission rates (7.0% vs 26.5%; P = 0.019), and a longer length of hospital stay (P = 0.03). Mixed infections in younger children were associated with a longer hospital stay (P = 0.023). Our results demonstrate the recent changes in the trends of circulating HAdV genotypes associated with ALRTIs in Hunan China.

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Molecular characterization of human adenoviruses in urban wastewaters using next generation and Sanger sequencing

Cited by 53 publications

References 47 publications

Detection of Human Bocavirus Species 2 and 3 in Bivalve Shellfish in Italy

Detection of Human Bocavirus Species 2 and 3 in Bivalve Shellfish in Italy

Molecular characterization of human Sapovirus in untreated sewage in Italy by amplicon-based Sanger and next-generation sequencing

Epidemiology of human adenovirus infection in children hospitalized with lower respiratory tract infections in Hunan, China

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