Molecular characterization of highly pathogenic Eimeria species among beef cattle on Java Island, Indonesia

Ekawasti, Fitrine; Nurcahyo, Wisnu; Wardhana, April Hari; Shibahara, Tomoyuki; Tokoro, Masaharu; Sasai, Kazumi; Matsubayashi, Makoto

doi:10.1016/j.parint.2019.101927

Cited by 35 publications

(84 citation statements)

References 22 publications

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“…The prevalence of calves over 6 months old is higher than that of calves 1–6 months old, which may be related to good care and colostrum feeding, especially colostrum can improve the immunity of calves. In addition, 18-month-old cattle are also unlikely to be infected with bovine Eimeria due to their highly immunity [ 3 , 22 , 25 , 37 , 57 ] – this is consistent with our findings. The eimeriosis prevalence of calves before weaning (<2 months) is significantly lower than that of calves after weaning (2–12 months), which may occur because calves acquire antibodies from breast milk [ 23 ].…”

Section: Discussionsupporting

confidence: 92%

Prevalence and infection risk factors of bovine Eimeria in China: a systematic review and meta-analysis

Gong

et al. 2021

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Eimeria spp. cause the disease coccidiosis, which results in chronic wasting of livestock and can lead to the death of the animal. The disease, common worldwide, has caused huge economic losses to the cattle industry in particular. This is the first systematic review and meta-analysis of the prevalence of bovine Eimeria in China. Our search of five databases including PubMed, ScienceDirect, China National Knowledge Infrastructure (CNKI), Chongqing VIP, and Wan Fang for articles published up to February 29, 2020 on the prevalence of Eimeria in cattle in mainland China yielded 46 articles, in which the prevalence of cattle ranged from 4.6% to 87.5%. The rate of bovine Eimeria infection has been decreasing year by year, from 57.9% before 2000 to 25.0% after 2015, but it is still high. We also analyzed the region, sampling years, detection methods, feeding model, seasons, and species of bovine Eimeria. We recommend that prevention strategies should focus on strengthening detection of Eimeria in calves in the intensive farming model.

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Section: Discussionsupporting

confidence: 92%

Prevalence and infection risk factors of bovine Eimeria in China: a systematic review and meta-analysis

Gong

et al. 2021

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“…It was also reported that diagnosis based on the qualitative identification of oocysts in feces using the sugar flotation modified method was highly sensitive for detecting Eimeria spp. [12]. Indonesia is a tropical country that has a total beef cattle population of approximately 16.5 million, which is more than that of dairy cattle, whose population is only approximately 0.5 million [13].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and risk factors associated with Eimeria species infection in cattle of different geographical regions of Indonesia

et al. 2021

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Background and Aim: Eimeria spp. are gastrointestinal protozoans that affect animal productivity, thereby causing symptoms that range from bloody diarrhea to death. These symptoms cause economic losses to farmers. The distribution of Eimeria spp. in cattle has, therefore, been reported to have spread widely, especially in the tropics and subtropics. Indonesia is a tropical country at high risk of Eimeria infections. This study aimed to identify the prevalence and risk factors related to the levels of eimeriosis in beef cattle originating from different geographic areas in Indonesia. Materials and Methods: Here, 817 fecal samples were collected from beef cattle in Indonesia, including 282 calves, 535 adults, 530 males, and 287 females. In addition, 156 semi-intensively and 661 intensively managed cattle were randomly collected. Then, fecal samples were analyzed by parasitology examinations. Results: Screening examination using the sugar flotation modification method showed that Eimeria spp. were prevalent in Indonesia, as 65.4% of the bacterial strain was detected. The prevalence of identified Eimeria spp. in Indonesia was highest in North Maluku (Maluku Island) (94.1%), whereas the lowest levels were observed in West Java (24.0%) (Java Island). The prevalence was also found to be higher in males (79.3%) than females (51.9%). Similarly, levels in semi-intensively managed cattle (66.7%) were higher than those subjected to intensive management (65.9%). However, its prevalence in calf and adult cattle was similar. Conclusion: Bovine eimeriosis spp. were detected at high prevalence in Indonesia, and high-level risks were observed in infected males, including those under the semi-intensive management. In addition, although the results from oocyst examinations were based on qualitative analysis, the endemicity levels of Eimeria spp. among farms in Indonesia should be considered because Eimeria spp. were distributed in most parts of Indonesia. Based on the results of this study, we provide the first information about the prevalence of bovine eimeriosis from different geographical locations in Indonesia, which have differing climates associated with the level of the existing risk factors. Hence, farmers are advised to pay more attention to strict biosecurity techniques on their farms, thereby favoring the early control of bovine eimeriosis.

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“…All chickens were healthy without any clinical symptoms when fecal samples were collected. Each fecal sample was examined by the sugar flotation method [3] with the modification that the final flotation was conducted by settling for 30 min instead of centrifugation. Oocysts per gram (OPG) were determined using a plankton calculation slide (Matsunami Glass, Osaka, Japan).…”

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confidence: 99%

“…Fecal examinations were conducted by the Research Institute for Animal Science in Biochemistry and Toxicology (Kanagawa, Japan). Oocysts purified by the sugar floatation method were diluted in 1-2 ml PBS, and DNA was extracted as previously reported [3]. For identification of seven chicken Eimeria spp., PCR targeting the internal transcribed spacer-1 (ITS-1) region of ribosomal RNA gene was carried out as reported previously [6].…”

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confidence: 99%

Morphological and molecular identification of <i>Eimeria</i> spp. in breeding chicken farms of Japan

Matsubayashi

Shibahara

Matsuo

et al. 2020

The Journal of Veterinary Medical Science

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Molecular characterization of highly pathogenic Eimeria species among beef cattle on Java Island, Indonesia

Cited by 35 publications

References 22 publications

Prevalence and infection risk factors of bovine Eimeria in China: a systematic review and meta-analysis

Prevalence and infection risk factors of bovine Eimeria in China: a systematic review and meta-analysis

Prevalence and risk factors associated with Eimeria species infection in cattle of different geographical regions of Indonesia

Morphological and molecular identification of <i>Eimeria</i> spp. in breeding chicken farms of Japan

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