Molecular characterization of head and neck tumors by analysis of telomerase activity and a panel of microsatellite markers

Fiedler, Wolfgang; Hoppe, Constance; Schimmel, Bettina; Koscielny, Sven; Dahse, Regine; Bereczki, Zsuzsa; Claussen, Uwe; Ernst, Günther; Eggeling, Ferdinand von

doi:10.3892/ijmm.9.4.417

Cited by 6 publications

(8 citation statements)

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“…Some researches have revealed that ATM gene is a target for epigenetic silencing through aberrant methylation of its promoter region (Ai et al 2004;Bolt et al 2005;Vo et al 2004). Nevertheless, LOH and MSI, which are also the causes of reduced or absent ATM expression (Haidar et al 2000), are common molecular processes during the development and progression of HNSCC and also of other types of cancer (Fiedler et al 2002). However, whether there is LOH or MSI at ATM locus in OSCC or not is still yet to be known.…”

Section: Discussionmentioning

confidence: 98%

“…Several groups have identiWed the 11q22-23 locus where ATM locates as a common deletion site in head and neck squamous cell carcinoma (HNSCC) (Friedlander 2001;Fiedler et al (2002). Accumulated evidences have also indicated that ATM inactivation could be found in several kinds of sporadic carcinoma such as lymphoid malignancies (Pause et al 2004), breast carcinoma (Angèle et al 2000;Kairouz et al 1999), prostate cancer (Angèle et al 2004), ovarian cancer (Koike et al 1999), colorectal cancer (Grabsch et al 2006), non-small cell lung cancer and esophageal cancer (Jiang et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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ATM in oral carcinogenesis: association with clinicopathological features

Chen

2008

J Cancer Res Clin Oncol

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

ATM in oral carcinogenesis: association with clinicopathological features

Chen

2008

J Cancer Res Clin Oncol

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“…The analysis of LOH indicated the involvement of a variety of genes in the development and progression of LSCC (Rainho et al, 2001;dos Reis et al, 2002;Fiedler et al, 2002;Gunduz et al, 2002). It is hypothesised that LSCC develops after the accumulation of six to 10 independent genetic events (Renan, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…The MLH1 promoter region was analysed using two pairs of specific primers: MLH1-881 and MLH1-1219 or MLH1-881 and MLH1-1470 described elsewhere. (Papadimitrakopoulou, 2000;Rainho et al, 2001;Fiedler et al, 2002) PCR products were separated in nondenaturated 6% PAA gels, stained in ethidium bromide and directly visualised by UV illumination.…”

Section: Dna Amplification and Loh Analysismentioning

confidence: 99%

Impairment of MLH1 and CDKN2A in oncogenesis of laryngeal cancer

et al. 2004

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Our study aimed at elucidating which genetic alterations tend to form a network and could be applied as molecular markers of larynx squamous cell carcinoma (LSCC). A panel of genes involved in tumorigenesis was investigated. To search for the possible mechanisms of gene silencing, loss of heterozygosity (LOH) was analysed followed by testing DNA methylation and protein expression for those genes found with the highest frequency of LOH (CDKN2A (55.4%), MLH1 (46.0%), RB1 (35.7%)). A correlation of both LOH and hypermethylation with the loss of expression for CDKN2A and MLH1 was found. Disrupted Rb pathway (loss of expression of RB1 and/or of CDKN2A) in 55.9% of analysed cases confirmed the hypothesis that RB1 pathway is altered in head and neck squamous cell carcinomas, with CDKN2A (45%), rather than RB1 (11.8%) being more frequently inactivated. In LSCC, LOH tends to occur together in gene pairs or triplets. The pair MLH1/CDKN2A and triplets MLH1/TSG on 8p22/CDKN2A and MLH1/ CDKN2A/RB1 are related to staging and grading. LOH in MLH1 correlates with lower and LOH in CDKN2A with higher grades of LSCC. It can be concluded that MLH1 and CDKN2A play an important role in LSCC development and progression.

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“…33,34,36,45,48,55 Dysplastic regions in the head and neck region that show more LOH seem to be more at risk for neoplastic evolution. 17,18,19,33 There are several reports that LOH is associated with more advanced stage and more aggressive HNSCC tumors, 13,34,36,37,56 and specifically with nodal involvement. 32,44 Other authors describe very specific correlations such as a link between LOH at 17p and mitotic index, 39 a connection of LOH at MLH1 with lower grade HNSCC, and LOH at CDKN2A with higher grade, 9 or a preference of LOH in tumors originating from the pharynx.…”

Section: Lohmentioning

confidence: 99%

The clinical relevance of microsatellite alterations in head and neck squamous cell carcinoma: a critical review

Schutter¹,

Spaepen²,

Bride

et al. 2007

Eur J Hum Genet

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Triggered by the existing confusion in the field, the current paper aimed to review the current knowledge of both microsatellite instability (MSI) and loss of heterozygosity (LOH) detected by microsatellite markers in head and neck squamous cell carcinoma (HNSCC), and to provide the reader with an assessment of their prognostic and predictive value in this tumor type. For both MSI and LOH, various detection methods were included such as mono-and polynucleotidemarkers and gel-as well as automated analyses. Only studies based on PCR techniques with microsatellite markers were considered. Taking the methodological problems occurring in investigations with microsatellite markers into account, LOH seems to be more common than MSI in HNSCC. Although both types of microsatellite alterations have been correlated with clinicopathological features of this tumor type, only LOH seems to have a clear prognostic value. The predictive value of both MSI and LOH is debatable. More research has to be performed to clearly establish LOH detection as a translational application in the HNSCC field, aiming to predict response to treatments or outcome, and eventually to use as a therapeutic target.

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Molecular characterization of head and neck tumors by analysis of telomerase activity and a panel of microsatellite markers

Cited by 6 publications

References 0 publications

ATM in oral carcinogenesis: association with clinicopathological features

ATM in oral carcinogenesis: association with clinicopathological features

Impairment of MLH1 and CDKN2A in oncogenesis of laryngeal cancer

The clinical relevance of microsatellite alterations in head and neck squamous cell carcinoma: a critical review

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