Molecular characterization of group A rotavirus before and after the introduction of vaccines in Brazil

Abstract

Introduction: In this study, the molecular characteristics of group A rotavirus (RVA) were compared in samples obtained before and after RVA vaccine-introduction in Brazil. Methods: Eighty samples were screened for the presence of RVA. Positive samples were molecularly analyzed. Results: RVA positivity was 16.9%, with a predominance of G2P [4]. Periods: pre-vaccination: predominance of IId (G1), IId (G2) lineages, and I1 and E1 genotypes; post-vaccination: predominance of Ib (G1), IIa, and IIc (G2) lineages and I2 and E2 genotypes. Conclusions: Although changes in RVA-circulation pattern were observed in the post-vaccination period, it could not be attributed to vaccination process.

Molecular characterization of rotavirus group A strains circulating prior to vaccine introduction in rural coastal Kenya, 2002-2013

“…Such a scenario was observed in Japan where, a G8P[8] RVA strain appeared to emerge and was found in up to 66% (53/80) of children with acute gastroenteritis disease attending one pediatric clinic in Shizuoka Prefecture in February – July 2017 10 . Similarly, the predominance of non-vaccine type G2P[4] strains was observed in Rotarix® vaccinated populations of Belgium 11 and Brazil 12 . Although the rise was not linked to pressure induced by the vaccine, concerns still remain on the overall effect of the vaccine on circulating vaccine heterotypic strains.…”

Circulating Profile of Rotavirus A, G and P Genotypes Before and After Vaccine Introduction in the Brazilian Mid-West 1986-2015

“…Worldwide G1 and P [8] have been the predominant genotypes (Santos & Hoshino, 2005), being replaced at regular intervals, especially by G2 and P [4] ( Dóró et al, 2014), as well as other emerging genotypes such as G5 and G9 (Santos & Hoshino, 2005). Many studies of the genotypic profile of RVA, in the Brazilian Mid-West, have been conducted since 1986, detecting the occurrence of distinct G and P genotypes with changes over time, notably G1P [8], G2P [4] and G9P [8] (Cardoso et al 2000, Souza et al 2003, Costa et al 2004, Andreasi et al 2007, Munford et al 2009, Borges et al 2011, Almeida et al 2015.…”

“…Studies conducted in the Brazilian Mid-West have shown a temporal variation in the predominance of certain G and P genotypes, characterizing the occurrence of an antigenic shift (Cardoso et al, 2000, Souza et al, 2003, Costa et al, 2004, Andreasi et al, 2007, Munford et al, 2009, Borges et al, 2011, Almeida et al, 2015.…”

“…In order to analyze the circulation of G and P genotypes in the Brazilian Mid-West, data was compared from studies conducted in the Mid-West in the pre-vaccine period (Cardoso et al, 2000, Souza et al, 2003, Costa et al, 2004, Andreasi et al, 2007, Munford et al, 2009, and post-vaccine period (Munford et al, 2009, Borges et al, 2011, Almeida et al, 2015, with data from samples obtained in the period from 2014 to 2015.…”

“…In previous studies, fecal samples were collected from children with AGE (Souza et al, 2003, Costa et al, 2004, Andreasi et al, 2007, Munford et al, 2009, Almeida et al, 2015, without AGE (Borges et al, 2011), and from children with or without AGE (Cardoso et al, 2000). These studies included hospitalized children (Souza et al, 2003, Costa et al, 2004, Andreasi et al, 2007, Munford et al, 2009, Almeida et al, 2015, hospitalized and seen in outpatient clinics (Cardoso et al, 2000) and children that attended day-care centers (Borges et al, 2011).…”