Molecular characterization of glyoxalase-I from a higher plant; upregulation by stress

Espartero, Joaquín; Sánchez‐Aguayo, Inmaculada; Pardo, José M.

doi:10.1007/bf00020464

Cited by 161 publications

(108 citation statements)

References 28 publications

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“…Under stress conditions, the increased level of methylglyoxal could further lower the glutathione level required for scavenging of reactive oxygen species, which increase under stress conditions [25]. Since the glyoxalase I has been shown to be upregulated under different abiotic stresses [14,15] and an increase in the glyoxalase II transcript has been observed under various abiotic stresses in Brasssica juncea [17] and in rice [18], the overexpression of the enzymes of the glyoxalase system is presumed to be involved in stress tolerance by detoxifying methylglyoxal and elevating the level of glutathione. This has been recently demonstrated in transgenic tobacco plants overexpressing the glyoxalase I and glyoxalase II gene.…”

Section: Resultsmentioning

confidence: 99%

“…An inverse correlation between glyoxalase I activity and differentiation in vitro was also observed in plants [12,13]. Glyoxalase I from tomato and Brassica juncea has been shown to be upregulated under different abiotic stresses [14,15]. Moreover; the overexpression of glyoxalase I from B. juncea has been shown to impart tolerance in tobacco plants under salinity and heavy metal stress [15].…”

Section: Introductionmentioning

confidence: 99%

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GOverexpression of the Glyoxalase II Gene Leads to Enhanced Salinity Tolerance in Brassica Juncea

Saxena¹

2011

TOPSJ

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Engineering of salinity tolerance in agronomically important crop plants is required to increase their productivity by enabling them to grow in saline soils, which are otherwise left uncultivated. Since an increase in the enzymes of glyoxalase system has been shown to impart salinity tolerance in the model plant tobacco, we used the glyoxalase II gene for engineering salinity tolerance in an important oil yielding crop, Brassica juncea. The transgenic plants of B. juncea overexpressing the glyoxalase II gene showed higher salinity tolerance as compared to the untransformed control plants as observed by delayed senescence in leaf discs at 400 mM and 800 mM NaCl in T1 generation. The percentage of germination of the T2 transgenic seeds was higher at 150 mM and 200 mM NaCl as compared to the seeds of untransformed plants. This for the first time demonstrates the applicability of utilizing the glyoxalase II gene for enhanced salinity tolerance in an oilseed crop plant B. juncea.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

GOverexpression of the Glyoxalase II Gene Leads to Enhanced Salinity Tolerance in Brassica Juncea

Saxena¹

2011

TOPSJ

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“…The physiological significance of the glyoxalase system has not been clearly defined in plants; however, this system has been often regarded as a ''marker for cell growth and division'' (11). Gly I has been shown to be up-regulated in tomato in response to salt and osmotic stress and to phytohormonal stimuli (12).…”

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confidence: 99%

Genetic engineering of the glyoxalase pathway in tobacco leads to enhanced salinity tolerance

Singla‐Pareek

Reddy²,

Sopory³

2003

Proc. Natl. Acad. Sci. U.S.A.

355

259

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The glyoxalase pathway involving glyoxalase I (gly I) and glyoxalase II (gly II) enzymes is required for glutathione-based detoxification of methylglyoxal. We had earlier indicated the potential of gly I as a probable candidate gene in conferring salinity tolerance. We report here that overexpression of gly I؉II together confers improved salinity tolerance, thus offering another effective strategy for manipulating stress tolerance in crop plants. We have overexpressed the gly II gene either alone in untransformed plants or with gly I transgenic background. Both types of these transgenic plants stably expressed the foreign protein, and the enzyme activity was also higher. Compared with nontransformants, several independent gly II transgenic lines showed improved capability for tolerating exposure to high methylglyoxal and NaCl concentration and were able to grow, flower, and set normal viable seeds under continuous salinity stress conditions. Importantly, the double transgenic lines always showed a better response than either of the single gene-transformed lines and WT plants under salinity stress. Ionic measurements revealed higher accumulation of Na ؉ and K ؉ in old leaves and negligible accumulation of Na ؉ in seeds of transgenic lines as compared with the WT plants. Comparison of various growth parameters and seed production demonstrated that there is hardly any yield penalty in the double transgenics under nonstress conditions and that these plants suffered only 5% loss in total productivity when grown in 200 mM NaCl. These findings establish the potential of manipulation of the glyoxalase pathway for increased salinity tolerance without affecting yield in crop plants.

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“…When plant exposed to stress conditions, enzymes of glycolysis and TCA cycles cells showed increased activity due to rapid functioning of cell (Umeda et al, 1994;Espartero et al, 1995;Sommer et al, 2001) and as a result increased triose phosphates are converted to MG instead of pyruvate only. So higher amount of methylglyoxal production under drought and salinity stresses confirmed by , Alam et al (2014) and Nahar et al (2015).…”

Section: Figmentioning

confidence: 99%

“…During conversion of glyceraldehyde 3-phosphate (G3P) from dihydroxyacetone phosphate (DHAP) in glycolysis, MG is formed spontaneously in plants by non-enzymatic mechanisms under physiological conditions (Espartero et al, 1995;Yadav et al, 2005a). The rate of glycolysis increases under stress conditions, leading to an imbalance (in the initial and latter five reactions) in the pathway.…”

Section: Introductionmentioning

confidence: 99%

Salinity and drought-induced methylglyoxal detoxification in Brassica spp. and purification of a high active glyoxalase I from tolerant genotype

Hossain¹,

Hasanuzzaman²,

Hoque³

et al. 2016

Plant Omics

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This experiment was conducted to study the role of glyoxalase system in conferring salinity and drought stress in Brassica spp. Two Brassica genotypes viz. BARI Sharisha16 (tolerant) and Tori7 (susceptible) were exposed to salt (16 dS m -1 ) and drought for 2, 4 and 6 days. The comparative study of two Brassica genotypes under salinity and drought stresses revealed that BARI Sharisha16 is more tolerant than Tori7 in both stresses. Under drought stress and salinity stress, Gly I activity increased significantly in both genotypes. Notably, concomitant increased activities of Gly I and Gly II with increased methylglyoxal (MG) suggested their role in MG detoxification in Brassica Spp. At six-day of salt stress, it was remarkable that Gly I and Gly II activities were 49 and 36 % higher in BARI Sharisha16 than Tori7. In addition, Gly I and Gly II activities were 24 and 21 % higher in BARI Sharisha16 than Tori7 after sixth day of drought, and hence, using different column chromatography Gly I was purified from BARI Sharisha16 seedlings. In purification, the fraction eluted from affinity chromatography showed specific activity of 173.51 µM min -1 mg -1 protein. In SDS-PAGE, the purified Gly I protein migrated as a single band on with an apparent molecular mass of 27 kDa. In final purification, the recovery of Gly I activity was 0.38% along with purification fold 112.7. In this study, role of glyoxalase system in detoxification of MG was observed and subsequently, Gly I was purified from tolerant genotypes.

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Molecular characterization of glyoxalase-I from a higher plant; upregulation by stress

Cited by 161 publications

References 28 publications

GOverexpression of the Glyoxalase II Gene Leads to Enhanced Salinity Tolerance in Brassica Juncea

GOverexpression of the Glyoxalase II Gene Leads to Enhanced Salinity Tolerance in Brassica Juncea

Genetic engineering of the glyoxalase pathway in tobacco leads to enhanced salinity tolerance

Salinity and drought-induced methylglyoxal detoxification in Brassica spp. and purification of a high active glyoxalase I from tolerant genotype

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