Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai

Abstract

Fusarium head blight (FHB) is a destructive disease of wheat worldwide. FHB resistance genes from Sumai 3 and its derivatives such as Ning 7840 have been well characterized through molecular mapping. In this study, resistance genes in Wangshuibai, a Chinese landrace with high and stable FHB resistance, were analyzed through molecular mapping. A population of 104 F 2 -derived F 7 recombinant inbred lines (RILs) was developed from the cross between resistant landrace Wangshuibai and susceptible variety Alondra's'. A total of 32 informative amplified fragment length polymorphism (AFLP) primer pairs (EcoRI/MseI) amplified 410 AFLP markers segregating among the RILs. Among them, 250 markers were mapped in 23 linkage groups covering a genetic distance of 2,430 cM. In addition, 90 simple sequence repeat (SSR) markers were integrated into the AFLP map. Fifteen markers associated with three quantitative trait loci (QTL) for FHB resistance (P < 0.01) were located on two chromosomes. One QTL was mapped on 1B and two others were mapped on 3B. One QTL on 3BS showed a major effect and explained up to 23.8% of the phenotypic variation for type II FHB resistance.Abbreviations: cM: centimorgan; LOD: log likelihood ratio; RILs: recombinant inbred lines; AFLP: amplified fragment length polymorphisms; FHB: Fusarium head blight; SSR: simple sequence repeat

A fast-neutron induced chromosome fragment deletion of 3BS in wheat landrace Wangshuibai increased its susceptibility to Fusarium head blight

“…The deletion in Wangshuibai decreased PSS to about 23.2%. The result was in consistent with previous reports that in average Fhb1 contributed to about 23.8% FHB resistance of Wangshuibai (Zhang et al 2004). Therefore, despite of the complication of FHB resistance, we deduced that it was the deletion of Fhb1 in Wangshuibai which led to its increased susceptibility.…”

“…Chinese wheat landrace Wangshuibai, which is originally from the severe FHB epidemic areas of the middle and lower region of the Yangtze River Area of China, has also been recognized worldwide as an excellent FHB-resistant source with all three types of resistances (Lin et al 2004(Lin et al , 2006Yu et al 2008) and can be an alternative source of FHB resistance in breeding programs. Mapping studies indicated that the major QTL for FHB resistance in Wangshuibai was also located in 3BS and was flanked by markers Xbarc147 and Xgwm493 (Zhang et al 2004;Lin et al 2004;Mardi et al 2005;Jia et al 2005). This QTL could explain up to 23.8% of the phenotypic variation for the FHB resistance of Wangshuibai (Zhang et al 2004).…”

“…Mapping studies indicated that the major QTL for FHB resistance in Wangshuibai was also located in 3BS and was flanked by markers Xbarc147 and Xgwm493 (Zhang et al 2004;Lin et al 2004;Mardi et al 2005;Jia et al 2005). This QTL could explain up to 23.8% of the phenotypic variation for the FHB resistance of Wangshuibai (Zhang et al 2004). However, this QTL may carry a different allele from that of Sumai 3, which explained about 42% of the phenotypic variation for the FHB resistance of Sumai 3 (Anderson et al 2001).…”

Quantitative trait loci for resistance to Fusarium head blight in the Chinese wheat landrace Huangfangzhu

“…HFZ contributed all favorable alleles. The RIL with 11 HFZ alleles at the QTL on 3BS and 7AL displayed significantly lower percentages of 12 infected spikelets (PIS) than RIL without these alleles in both greenhouse and field 13 environments. HFZ combined several alleles from germplasm reported previously and 14 is a promising alternative source for improving wheat FHB resistance.…”

“…HFZ combined several alleles from germplasm reported previously and 14 is a promising alternative source for improving wheat FHB resistance. Growing resistant cultivars within an integrated cultural system is the most 11 economic, effective and environmentally safe approach to reducing losses caused by 12 this disease. Because environments significantly affect FHB response, large-scale 13 phenotypic selection for resistance is difficult and requires costly and laborious field 14 evaluations with poor repeatability among testing seasons and locations.…”