Molecular characterization of freshwater snails in the genus Bulinus: a role for barcodes?

Kane, Richard A.; Stothard, J. Russell; Emery, Aidan M.; Rollinson, David

doi:10.1186/1756-3305-1-15

Cited by 84 publications

(161 citation statements)

References 28 publications

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“…Accurate identification of such intermediate snail hosts is a prerequisite for understanding disease epidemiology and aids in focusing control, especially if resources are limited and parasites have a wide host range (Kane et al, 2008;Rollinson et al, 2009). Historically, species of Radix have been identified using shell morphology, reproductive system anatomy and colouration and these parameters continue to be used to describe new species (Glöer and Pešic, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Molecular-based taxonomy of disease vectors often contradicts traditional views and can result in extensive taxonomic revisions. For example several medically important snail species within the genus Bulinus were originally named and identified based on morphological characteristics, such as shell structure and internal anatomy, but have since been separated into distinct species based on molecular evidence (Kane et al, 2008). In fact, molecular approaches have aided in the elucidation of cryptic species and species complexes that appear morphologically identical (Pfenninger et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Such markers have included the nuclear ribosomal gene 18s, and the internal transcribed spacer regions ITS1 and ITS2 (Bargues et al, 2001) as well as the international barcoding gene cytochrome oxidase 1 (cox1) and ribosomal 16s (Correa et al, 2010;Pfenninger et al, 2011). Historically, both ITS regions have been used to differentiate between species of aquatic snail intermediate hosts of schistosomes that cause human urinary and hepatointestinal African schistosomiasis; including Bulinus and Biomphalaria, respectively (Dejong et al, 2003;Kane et al, 2008;Raahauge and Kristensen, 2000). However, Kane et al (2008) and Dejong et al (2003) argued that mitochondrial genes such as cox1 and 16s were more effective at differentiating species than nuclear genes, and could provide a biogeographical perspective because of their higher rate of evolution.…”

Section: Introductionmentioning

confidence: 99%

“…Historically, both ITS regions have been used to differentiate between species of aquatic snail intermediate hosts of schistosomes that cause human urinary and hepatointestinal African schistosomiasis; including Bulinus and Biomphalaria, respectively (Dejong et al, 2003;Kane et al, 2008;Raahauge and Kristensen, 2000). However, Kane et al (2008) and Dejong et al (2003) argued that mitochondrial genes such as cox1 and 16s were more effective at differentiating species than nuclear genes, and could provide a biogeographical perspective because of their higher rate of evolution. Cox1 has been used to differentiate between species and populations of snails including Indoplanorbis exustus (Liu et al, 2010), Theba pisana pisana (Däumer et al, 2012) and species of Pomacea (Hayes et al, 2008;Lv et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Unravelling the riddle of Radix: DNA barcoding for species identification of freshwater snail intermediate hosts of zoonotic digeneans and estimating their inter-population evolutionary relationships

Lawton

Lim

Dukes

et al. 2015

Infection, Genetics and Evolution

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Please cite this article as: Lawton, S.P., Lim, R.M., Dukes, J.P., Kett, S.M., Cook, R.T., Walker, A.J., Kirk, R.S., Unravelling the riddle of Radix: DNA barcoding for species identification of freshwater snail intermediate hosts of zoonotic digeneans and estimating their inter-population evolutionary relationships, Infection, Genetics and Evolution (2015), doi: http://dx.doi.org/10. 1016/j.meegid.2015.07.021 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. and ITS2 sequences, identified populations of Radix auricularia and R. balthica from specimens originally morphologically identified as R. peregra from the UK. Assessment of cox1 and ITS2 as species identification markers showed that, although both markers differentiated species, cox1 possessed greater molecular diversity and higher phylogenetic resolution. Cox1 also proved useful for gaining insights into the evolutionary relationships of Radix species populations. Phylogenetic analysis and haplotype networks of cox1 indicated that Radix auricularia appeared to have invaded the UK several times; some haplotypes forming a distinct UK specific clade, whilst others are more akin to those found on mainlandEurope. This was in contrast to relationships between R. balthica populations, which had low molecular diversity and no distinct UK specific haplotypes, suggesting recent and multiple invasions from mainland Europe. Molecular techniques therefore appear to be crucial for distinguishing Radix spp., particularly using cox1. This barcoding marker also enables the population biology of Radix spp. to be explored, and is invaluable for monitoring the epidemiology of fluke diseases especially in the light of emerging diseases and food security.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Unravelling the riddle of Radix: DNA barcoding for species identification of freshwater snail intermediate hosts of zoonotic digeneans and estimating their inter-population evolutionary relationships

Lawton

Lim

Dukes

et al. 2015

Infection, Genetics and Evolution

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“…The current study described the medically important snails detected for the first time in Miangran Lake as follows: Lymnaeidae snails with a diverse distribution and parasitological importance because they are intermediate hosts of some trematodes that infect human e g. Fasciola spp. (5,15), genus Bulinus which act as intermediate hosts for Schistosomes that are responsible for a highly significant group of infections in humans termed schistosomiasis (14). Genus Bithynia that is important because some species are intermediate hosts of liver and intestinal trematodes; for example, the liver fluke Opisthorchis viverrini (13), and Genus Melanoides that is considered to be of medical significance.…”

Section: Discussionmentioning

confidence: 99%

Identification of Medically Important Snails of Miangran Lake in Izeh, Khuzestan Province of Iran

Nouroozi¹

2015

Avicenna J Clin Microbiol Infect

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Background: Some freshwater snails are acting as intermediate hosts for digenetic trematodes. Studies on distribution of freshwater snails are important to determine the transmission patterns of the trematoda. Objectives: The current study aimed to identify medically important snails of Miangran Lake in Izeh, Khuzestan province, Iran. Materials and Methods: Sampling was conducted in fourteen sites around Miangran Lake in 2014. The collected samples were placed in plastic containers containing 70% ethyl alcohol, prior to consideration. The identification was carried out according to shell characteristics. Data were analyzed descriptively. Results: All sampling sites were positive for medically important snails. Overall, nine genera and thirteen species were identified. The most diversity in genus was found in Melanopsis. Five genera of snails detected in the study with known medical importance include: Bithynia spp., Bulinus spp., Lymnaea spp., Melanoides spp. and Melanopsis spp. Melanoides spp. was observed in thirteen and Bellamya spp. was identified in two sites. Also, in this study Melanoides spp., Bulinus spp., and Lymnaea spp. were widespread snails around Miangran Lake. Conclusions: The reason for difference in the detected snail genera in sampling sites may be due to various physicochemical factors. According to the current study, medically important snails exist in Miangran Lake and they could be a source of trematode infections for the local people. Controlling measures after comprehensive studies should be applied.

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Genome‐wide single‐nucleotide polymorphism data reveal cryptic species within cryptic freshwater snail species—The case of the Ancylus fluviatilis species complex

Weiss

Weigand

et al. 2017

Ecology and Evolution

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DNA barcoding utilizes short standardized DNA sequences to identify species and is increasingly used in biodiversity assessments. The technique has unveiled an unforeseeably high number of morphologically cryptic species. However, if speciation has occurred relatively recently and rapidly, the use of single gene markers, and especially the exclusive use of mitochondrial markers, will presumably fail in delimitating species. Therefore, the true number of biological species might be even higher. One mechanism that can result in rapid speciation is hybridization of different species in combination with polyploidization, that is, allopolyploid speciation. In this study, we analyzed the population genetic structure of the polyploid freshwater snail Ancylus fluviatilis, for which allopolyploidization was postulated as a speciation mechanism. DNA barcoding has already revealed four cryptic species within A. fluviatilis (i.e., A. fluviatilis s. str., Ancylus sp. A-C), but early allozyme data even hint at the presence of additional cryptic lineages in Central Europe. We combined COI sequencing with high-resolution genome-wide SNP data (ddRAD data) to analyze the genetic structure of A. fluviatilis populations in a Central German low mountain range (Sauerland). The ddRAD data results indicate the presence of three cryptic species within A. fluviatilis s. str. occurring in sympatry and even syntopy, whereas mitochondrial sequence data only support the existence of one species, with shared haplotypes between species. Our study hence points to the limitations of DNA barcoding when dealing with organismal groups where speciation is assumed to have occurred rapidly, for example, through the process of allopolyploidization. We therefore emphasize that single marker DNA barcoding can underestimate the true species diversity and argue in strong favor of using genome-wide data for species delimitation in such groups. K E Y W O R D Sgastropoda, mito-nuclear discordance, molecular species delimitation, RAD-seq

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Molecular characterization of freshwater snails in the genus Bulinus: a role for barcodes?

Cited by 84 publications

References 28 publications

Unravelling the riddle of Radix: DNA barcoding for species identification of freshwater snail intermediate hosts of zoonotic digeneans and estimating their inter-population evolutionary relationships

Unravelling the riddle of Radix: DNA barcoding for species identification of freshwater snail intermediate hosts of zoonotic digeneans and estimating their inter-population evolutionary relationships

Identification of Medically Important Snails of Miangran Lake in Izeh, Khuzestan Province of Iran

Genome‐wide single‐nucleotide polymorphism data reveal cryptic species within cryptic freshwater snail species—The case of the Ancylus fluviatilis species complex

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