Molecular Characterization of FLT3 Mutations in Acute Leukemia Patients

Abstract

Fms-like tyrosine kinase 3 (FLT3) performs a vital role in the pathogenesis of hematopoietic malignancies. Therefore in recent times, the focus of several studies was on use of FLT3 as a prognostic marker. The present study investigated the molecular characterization and incidence of FLT3 mutations in acute leukemia patients in Pakistan. A total of 55 patients were studied, of which 25 were suffering from acute lymphoblastic leukemia (ALL) and 30 were suffering from acute myeloid leukemia (AML). The polymerase chain reaction demonstrated FLT3/ ITD mutations in 1 (4%) of 25 ALL patients, a male with the L2 subtype. In AML cases the rate was 4 (13.3%) of 30, three males and one female. The AML-M4 subtype was found in three and the AML M2 subtype in the other. In the AML cases, a statistically significant (p=0.009) relationship was found between WBC (109/L) and FLT3/ ITD positivity. However, no significant relationship was found with other clinical parameters (p>0.05). In acute myeloid leukemia (AML) FLT3/ITD + mutation was more prevalent in elderly patients 31-40 age groups, 21-30 and 51-60 age groups respectively. In acute lymphoblastic leukemia (ALL) statistically no significant relationship was found between clinical features and FLT3/ITD positivity (p>0.05). However, in acute lymphoblastic leukemia (ALL) FLT3/ITD + mutation was more commonly found in age groups of 21-30.

Frequency and Prognostic Relevance ofFLT3Mutations in Saudi Acute Myeloid Leukemia Patients

“…In our study, the frequency of FLT3 -ITD mutation in adult AML was 14.43% which is lower than reported in other published studies [8, 19, 20, 22, 28, 35], but still in the variation range of frequency of ITD mutations (13–27%) reported by some study group [12, 21] (Table 2). In contrast to published studies the rate of FLT3 -ITD mutation in pediatric AML (20%) is more than the adult and higher than most of the reported studies [33, 34, 36].…”

“…One small study was done on 30 AML patients with frequency of FLT3 /ITD mutation in AML which was 4/30 (13.3%); three were males and one female [12]. However, these results should be treated with reservation, due to the relatively small sample sizes.…”

Staphylococcus Spp Bacteremia Complications in Acute Leukemia Patient After Chemotherapy

“…ALL-L1 is a more common morphological subtype might in our community because most of patients in our study were children 38/54 (70.4%) and according to FAB classification, ALL-L1 morphological subtype was the most common type in childhood accounts for over 80% of cases, whereas ALL-L2 morphological subtype was the most common type in adults, it constitute 11-14% of ALL cases in children. The mentioned results agree with some studies found that the percentage of ALL-L1 was higher 76% than other subtypes (Kamps demonstated that commonest morphological subtype in Erbil in Ishfaq et al (2012 were observed 68 %, 32% for L1 and L2, respectively, and disagree with (Al-Barazanch et al, 2005) who found that ALL more common type in both children and adults 87.2% and 92% respectively than L1 morphological subtype.…”

Detection of TET2 Mutation in Patients with De Novo Acute Myeloid Leukemia: A Mutation Analysis of 51 Iranian Patients

“…Metzeler et al report were the only study in line with our findings (Metzeler et al, 2011). Regarding our analyses, the TET2mut did not show any correlation with FLT3, NPM1 and CEBPA expression levels (Ishfaq et al, 2012;Sarojam et al, 2015;Alarbeed et al, 2021). Since the previous studies evaluated the molecular prognostic markers through the sequencing method, this discrepancy might be caused by the difference in method.…”