Molecular Characterization of Extended-Spectrum β-Lactamases Produced by Nosocomial Isolates of
            <i>Enterobacteriaceae</i>
            from an Italian Nationwide Survey

Perilli, Mariagrazia; Dell'Amico, Emanuela; Segatore, Bernardetta; Massis, Maria Rosaria De; Bianchi, Ciro; Luzzaro, Francesco; Rossolini, Gian María; Toniolo, Antonio; Nicoletti, Giuseppe; Amicosante, Gianfranco

doi:10.1128/jcm.40.2.611-614.2002

Cited by 125 publications

(111 citation statements)

References 25 publications

(29 reference statements)

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“…In addition, they may be resistant to other groups of antimicrobial agents [18]. Because ESBL-producing enterobacteriaceae frequently cause urinary tract infections, cross resistance with fluoroquinolones is a very challenging issue since intravenous therapy is not recommended in these infections except for the most serious (Pyelonephritis).…”

Section: Discussionmentioning

confidence: 99%

“…For instance, if a strain contained blaTEM and the IEF results showed a betalactamase with a pI of 5.4, an assumption was made that this beta-lactamase most likely represented the non-ESBL TEM-1 and the other gene was sequenced. All strains were examined for the presence of blaTEM, blaSHV, blaCTX-M, and blaOXA-1 using PCR conditions as previously described [16,18].The sequence of the blaTEM, blaSHV, and blaCTX-M ORF PCR products were determined by using a Big Dye PCR (Perkin-Elmer/Cetus) and analyzed on an ABI Prism 3130 DNA Genetic Analyzer (PerkinElmer Applied Biosystems, Foster City, USA).…”

Section: Pcr Detection and Sequencing Of Blatem Blashv Blactx-m Anmentioning

confidence: 99%

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Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae

Daoud

Sokhn

Azar

et al. 2014

J Infect Dev Ctries

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Introduction: The aim of this work was to study the ability of ciprofloxacin to restrict the development of resistant mutants of Escherichia coli and Klebsiella pneumoniae through determination of the Mutant Prevention Concentration (MPC). Methodology: We studied 140 strains of E. coli and 86 strains of K. pneumoniae with different profiles of sensitivity to fluoroquinolones and extended spectrum beta lactamase (ESBL) production. The MPCs were determined using an inoculum of 10 10 CFU/ml in Mueller-Hinton agar plates with serial concentrations of ciprofloxacin. Results: Ciprofloxacin-susceptible ESBL-producing strains showed a higher MPC for ciprofloxacin (P <0.001) than ciprofloxacin-susceptible non ESBL-producing strains, while ciprofloxacin-resistant ESBL-producing and non ESBL-producing strains did not significantly differ. The presence of qnr variants was associated with elevated MPCs. This was observed for both tested organisms.

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Section: Discussionmentioning

confidence: 99%

Section: Pcr Detection and Sequencing Of Blatem Blashv Blactx-m Anmentioning

confidence: 99%

Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae

Daoud

Sokhn

Azar

et al. 2014

J Infect Dev Ctries

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“…The SHV ESBL are prevalent in USA, Europe and Latin America. TEM type ESBLs have been found to be predominant in Italy and France [18] . The CTX-M-encoding genes are believed to have been captured from the chromosome of Kluyvera spp.…”

Section: Arjuna Camellia Sinensis Holarrhena Antidysentrica ---------mentioning

confidence: 99%

“…Antimicrobial assay was done by agar well dilution method [17,18] with control. Test organisms were diluted to 0.1 mL and spread on MHA plates.…”

Section: Plant Activity Against Esbl Producing Organismsmentioning

confidence: 99%

Identification of Clonal Clusters of Klebsiella pneumoniae Isolates from Chennai by Extended Spectrum Beta Lactamase Genotyping and Antibiotic Resistance Phenotyping Analysis

Kamatchi¹,

Magesh²,

Sekhar³

et al. 2009

American J. of Infectious Diseases

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Problem statement: An increased resistance to antibiotics has been reported in Klebsiella pneumoniae, an opportunistic gram negative pathogen belonging to Entrobacteraceae due to the evolution and spread of plasmid encoded extended spectrum beta lactamases and other genes conferring cross-resistance to other antibiotics. This is of concern due to the increasing cost of antibiotic treatment and the spread of multidrug resistance to more pathogenic microorganisms. This study was undertaken to analyze the extent of the problem, to identify the most prevalent MDR isolates of Klebsiella pneumoniae in Chennai and to identify new plant based drugs. Approach: About 188 clinical isolates of Klebsiella pneumoniae were collected from Chennai during the period Nov. 2007-Oct. 2008 and their resistance to different groups of antibiotics were analyzed. These isolates were further characterized by molecular studies to identify the ESBL genes conferring the resistance phenotype. Plant extracts were tested against the MDR isolates to identify new treatment methods. Results: Our results showed that the combination therapy of clavulanic acid with cephalosporins and fluroroquinolones-norfloxacin and ciprofloxacin were the most effective antibiotics for treatment of Klebsiella pneumoniae infections. However resistance to clavulanic acid was increasing among the isolates (17%). All the isolates harbored a plasmid containing one or more of the genes for ESBL resistance. Plasmid isolation from the Isolates followed by ESBL PCR genotyping showed that CTX-M was present in 75 % isolates and TEM in 73% isolates either alone or in combination with the other ESBL genes. SHV ESBL type was present only in 42% of isolates. We identified 4 presumptive clonal spreads which were likely to be prevalent in India by clustering based on ESBL genotypic and antibiotic resistance. The isolates with both SHV and CTX were correlated with the most pathogenic and clavulanic acid resistant isolates. The extracts of Hemidesmus indicus and Terminalia arjuna had a significant antimicrobial activity against the MDR isolates. Conclusion: There was an increase in the spread of the ESBL genes and clavulanic acid resistance in the clinical isolates of Klebsiella pneumoniae in Chennai. Our data also supported a higher incidence of CTX-M genotype in India though the SHV genotype was associated with the most resistant forms of ESBL. Future research with separation of plant extracts to identify bioactive principles will pave the way for newer plant based antibiotics.

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“…Since 1996, only one isolate of TEM-52-producing E. coli has been identified in France during a national hospital survey (8). In 1997 and 1999, two studies demonstrated TEM-52 to be the most prevalent TEM-type ESBL among E. coli strains in Korea and among enterobacterial species in Italy (15,16). The first report of TEM-52 in the genus Salmonella described two strains isolated in 1998 from a hospitalized Yugoslavian infant (20).…”

mentioning

confidence: 99%

Extended-Spectrum-β-Lactamase (TEM-52)-Producing Strains of Salmonella enterica of Various Serotypes Isolated in France

et al. 2004

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Molecular Characterization of Extended-Spectrum β-Lactamases Produced by Nosocomial Isolates of Enterobacteriaceae from an Italian Nationwide Survey

Cited by 125 publications

References 25 publications

Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae

Mutant prevention concentrations of ciprofloxacin against urinary isolates of Escherichia coli and Klebsiella pneumoniae

Identification of Clonal Clusters of Klebsiella pneumoniae Isolates from Chennai by Extended Spectrum Beta Lactamase Genotyping and Antibiotic Resistance Phenotyping Analysis

Extended-Spectrum-β-Lactamase (TEM-52)-Producing Strains of Salmonella enterica of Various Serotypes Isolated in France

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