Molecular Characterization of Extended-Spectrum-Cephalosporin-Resistant Enterobacteriaceae from Wild Kelp Gulls in South America

Liakopoulos, Apostolos; Olsén, Björn; Geurts, Yvon; Artursson, Karin; Berg, Charlotte; Mevius, Dik; Bonnedahl, Jonas

doi:10.1128/aac.01120-16

Cited by 29 publications

(18 citation statements)

References 22 publications

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“…The role of wildlife in the dissemination of resistant genes is supported by the recent isolation of ESBL‐producing bacteria carrying bla CTX‐M‐15 genes in wild birds of Nicaragua, Chile and Argentina, and raises public health concerns given the implications of these bacteria in both nosocomial and community‐acquired infections in humans (Guenther et al., ; Hasan et al., ; Liakopoulos et al., ). Our findings highlight the need to elucidate the role of bats and livestock in AMR bacterial transmission cycles and what risks these community sources may pose for zoonotic transmission to humans.…”

Section: Discussionmentioning

confidence: 99%

Extended‐spectrum beta‐lactamase‐producing Escherichia coli in common vampire bats Desmodus rotundus and livestock in Peru

Benavides

Shiva

Virhuez

et al. 2018

Zoonoses and Public Health

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Antibiotic resistance mediated by bacterial production of extended-spectrum beta-lactamase (ESBL) is a global threat to public health. ESBL resistance is most commonly hospital-acquired; however, infections acquired outside of hospital settings have raised concerns over the role of livestock and wildlife in the zoonotic spread of ESBL-producing bacteria. Only limited data are available on the circulation of ESBL-producing bacteria in animals. Here, we report ESBL-producing Escherichia coli in wild common vampire bats Desmodus rotundus and livestock near Lima, Peru. Molecular analyses revealed that most of this resistance resulted from the expression of bla genes carried by plasmids, which are disseminating worldwide in hospital settings and have also been observed in healthy children of Peru. Multilocus sequence typing showed a diverse pool of E. coli strains carrying this resistance that were not always host species-specific, suggesting sharing of strains between species or infection from a common source. This study shows widespread ESBL resistance in wild and domestic animals, supporting animal communities as a potential source of resistance. Future work is needed to elucidate the role of bats in the dissemination of antibiotic-resistant strains of public health importance and to understand the origin of the observed resistance.

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Section: Discussionmentioning

confidence: 99%

Extended‐spectrum beta‐lactamase‐producing Escherichia coli in common vampire bats Desmodus rotundus and livestock in Peru

Benavides

Shiva

Virhuez

et al. 2018

Zoonoses and Public Health

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“…Like other gull species, kelp gulls are extremely opportunistic and generalist feeders, very adapted to exploiting a wide variety of human-impacted and highly populated areas, and food subsidies (e.g., fishing discards and refuse disposals) ( 40 – 42 ). Such behaviors have been associated with the presence of ARGs in kelp gulls in Argentina ( 43 ), as well as in other gull species worldwide ( 17 , 44 , 45 ). Conversely, the Magellanic penguin is a migratory upper trophic level predator and the most abundant penguin in temperate areas, widely distributed along the southern coast of South America ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

Occurrence and Quantification of Antimicrobial Resistance Genes in the Gastrointestinal Microbiome of Two Wild Seabird Species With Contrasting Behaviors

Ewbank

Esperón²,

Sacristán

et al. 2021

Front. Vet. Sci.

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Antimicrobial resistance genes (ARGs) are environmental pollutants and anthropization indicators. We evaluated human interference in the marine ecosystem through the ocurrence and quantification (real-time PCRs) of 21 plasmid-mediated ARGs in enema samples of 25 wild seabirds, upon admission into rehabilitation: kelp gull (Larus dominicanus, n = 14) and Magellanic penguin (Spheniscus magellanicus, n = 11). Overall, higher resistance values were observed in kelp gulls (non-migratory coastal synanthropic) in comparison with Magellanic penguins (migratory pelagic non-synanthropic). There were significant differences between species (respectively, kelp gull and Magellanic penguin): ARGs occurrence (blaTEM [p = 0.032]; tetM [p = 0.015]; tetA [p = 0.003]; and sulII [p = 0.007]), mean number of ARGs per sample (p = 0.031), ARGs mean load percentage (aadA [p = 0.045], tetA [p = 0.031], tetM [p = 0.016], blaTEM [p = 0.032], sulII [p = 0.008]), percentage of genes conferring resistance to an antimicrobial class (betalactams [p = 0.036] and sulfonamides [p = 0.033]), mean number of genes conferring resistance to one or more antimicrobial classes (p = 0.024]), percentage of multiresistant microbiomes (p = 0.032), and clustering (p = 0.006). These differences are likely due to these species' contrasting biology and ecology - key factors in the epidemiology of ARGs in seabirds. Additionally, this is the first report of mecA in seabirds in the Americas. Further studies are necessary to clarify the occurrence and diversity of ARGs in seabirds, and their role as potential sources of infection and dispersal within the One Health chain of ARGs.

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“…Kelp gulls along the Patagonian coast, in closer proximity to humans and domestic animals, have previously been shown to harbour Yersinia enterocolitica bacteria at low prevalences (1 positive sample out of 60), and also other pathogen microbes [24]. Similarly, Kelp gulls in Ushuaia in southern Argentine, have been demonstrated to harbour antibiotic resistant Enterobacteriaceae including a variety of Extended-spectrum β-lactamaseproducing strains [26].…”

Section: Arctic/antarctic Regionmentioning

confidence: 99%

Occurrence ofYersinia rohdeiamong feral reindeer (Rangifer t. tarandus) and kelp gulls (Larus dominicanus) on the Sub-Antarctic island South Georgia

Bonnedahl

Berg

Andersson

et al. 2018

Infection Ecology & Epidemiology

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Introduction: During a research expedition in 2012, faecal samples were collected from feral reindeer and kelp gulls on the main island of South Georgia in the Sub-Antarctic region of the Atlantic. The samples were analysed for bacteria of the genus Yersinia with the aim of identifying isolates to the species level. Materials and Methods: A total of 11 reindeer samples and 26 Kelp Gull samples were retrieved from the location of Stromness, kept refrigerated and cultivated for gram-negative bacteria. Results: Three of the samples showed growth of Yersinia rohdei, as confirmed by biochemical typing, MALDI-TOF and 16S rRNA sequencing. The isolates were indistinguishable from each other by 16S sequencing, and differed by a single base pair from the type strain of Y. rohdei. Discussion: The Yersinia genus contains well-known pathogens of significance to both human and veterinary medicine, but the public health and animal health relevance of Y. rohdei is unknown. Although it is clear that Y. rhodei is present in the south Georgian biotope, its importance and relevance for biological diversity is unknown, as is if this presence is merely a reflection of human activities.

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Molecular Characterization of Extended-Spectrum-Cephalosporin-Resistant Enterobacteriaceae from Wild Kelp Gulls in South America

Cited by 29 publications

References 22 publications

Extended‐spectrum beta‐lactamase‐producing Escherichia coli in common vampire bats Desmodus rotundus and livestock in Peru

Extended‐spectrum beta‐lactamase‐producing Escherichia coli in common vampire bats Desmodus rotundus and livestock in Peru

Occurrence and Quantification of Antimicrobial Resistance Genes in the Gastrointestinal Microbiome of Two Wild Seabird Species With Contrasting Behaviors

Occurrence ofYersinia rohdeiamong feral reindeer (Rangifer t. tarandus) and kelp gulls (Larus dominicanus) on the Sub-Antarctic island South Georgia

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