Molecular characterization of Enterobacteriacea isolated from gingivitis and periodontitis patients and the antimicrobial activity of mouth wash agents

Mohammed, D I; Moses, I B; Oyibo, A.O.; Ibitayo, O.O.; Ifeanyichukwu, Iroha

doi:10.1016/j.sciaf.2022.e01106

Cited by 3 publications

(14 citation statements)

References 24 publications

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“…Non-oral bacteria are transient or non-resident pathogenic bacteria that are not generally considered a common part of the oral microbiota [1]. The oral microbiota has been reported to contain more than one hundred thousand (1000) species of bacteria [2,3,4] belonging to the genera Streptococcus, Enterococcus, Staphylococcus, Actinomyces, Peptostreptococcus, Eubacterium, Treponema, Corynebacterium, Bacteroides, Lactobacillus, Fusobacterium, Leptotrichia, Campylobacter, Prophyromonas, etc., [5,6] with only a few proportions of these bacteria are associated with dental disease such as periodontitis, gingivitis, dental caries, etc., [7,8]. However, the invasion and colonization of the oral cavity by non-oral bacteria such as Staphylococci, enterococci and Gram-negative enteric rods (GNRs) depict an imbalance of oral flora in the oral cavity [6,9,10].…”

Section: Introductionmentioning

confidence: 99%

“…The oral microbiota has been reported to contain more than one hundred thousand (1000) species of bacteria [2,3,4] belonging to the genera Streptococcus, Enterococcus, Staphylococcus, Actinomyces, Peptostreptococcus, Eubacterium, Treponema, Corynebacterium, Bacteroides, Lactobacillus, Fusobacterium, Leptotrichia, Campylobacter, Prophyromonas, etc., [5,6] with only a few proportions of these bacteria are associated with dental disease such as periodontitis, gingivitis, dental caries, etc., [7,8]. However, the invasion and colonization of the oral cavity by non-oral bacteria such as Staphylococci, enterococci and Gram-negative enteric rods (GNRs) depict an imbalance of oral flora in the oral cavity [6,9,10]. The presence and proliferation of non-oral bacteria in the oral cavity have been associated with several oral diseases such as caries, periodontitis, gingivitis, and more systemic diseases such as rheumatoid arthritis, endocarditis and cystic fibrosis [1,6].…”

Section: Introductionmentioning

confidence: 99%

“…However, the invasion and colonization of the oral cavity by non-oral bacteria such as Staphylococci, enterococci and Gram-negative enteric rods (GNRs) depict an imbalance of oral flora in the oral cavity [6,9,10]. The presence and proliferation of non-oral bacteria in the oral cavity have been associated with several oral diseases such as caries, periodontitis, gingivitis, and more systemic diseases such as rheumatoid arthritis, endocarditis and cystic fibrosis [1,6].…”

Section: Introductionmentioning

confidence: 99%

“…Within the oral cavity, one of the most common groups of bacteria that are of medical importance in healthcare today is Gram-negative bacteria, which together with other highly important MDR Gram-positive pathogens of the non-oral cavity. In dental disease patients, the eradication of this non-oral bacteria from the dental plaque or biofilm seems to be more challenging due to their high MDR profile to antimicrobial agents has raised the probability of treatment failure and reinfection [6,19].…”

Section: Introductionmentioning

confidence: 99%

“…Generally, data about the prevalence of antibiotic resistance on non-oral bacteria are difficult to find, particularly in countries where antibiotics are easily obtainable Over The Counter (OTC). Despite the untenable rate of antibiotic-resistant bacterial infections reported in dentistry in most published studies in Nigerian [4,6,20,21], there is a substantial gap in the surveillance of these non-oral bacteria in several Nigerian cities especially in Southeastern Nigeria where limited studies have been done on the prevalence of resistant oral and non-oral bacteria [4,6]. Hence, it worthwhile investigating the antibiotic resistance of non-oral bacteria that inhabit or colonizes the oral cavity among patients attending FCDTTEN to optimize treatment and decrease mortality rates.…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic Characterization and Antibiogram of Non-Oral Bacteria Isolates from Patients Attending Dental Clinic at Federal College of Dental Technology and Therapy Medical Center Enugu

Edemekong

Iroha²,

Thompson³

et al. 2022

IJPR

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Background and Objectives: Antibiotic-resistance among microbiota found within the oral cavity is a growing concern due to extensive use of antibiotics in dental practice both for therapeutic and prophylactic reasons, but has so far received little attention in recent time. The aim of this study was to determine the antibiogram of non-oral bacteria isolates from patients attending dental clinic at Federal College of Dental Technology and Therapy Medical Center Enugu (FEDCODTTEN) Methodology: A total of two hundred (200) oral swab samples were collected from patients with dental disease, placed in sterilized Brain Heart Infusion broth and immediately transported to the Microbiology Laboratory Unit of Federal College of Dental Technology and Therapy Enugu, for bacteriological analysis using standard microbiological methods for isolation and characterization. Antibiogram studies of non-oral bacteria was performed using the Kirby–Bauer disk diffusion method and the results were interpreted using the Clinical Laboratory Standard Institute (CLSI) zone diameter breakpoints. Multiple antibiotic resistance index (MARI) was determined for Multidrug Resistant (MDR) non-oral bacteria. Results: Phenotypic characterization of non-oral bacteria revealed an occurrence rate of S. aureus 35(17.5%) followed by E. coli 18(9.0%), Salmonella typhi 16(8.0 %) and K. oxytoca 4(2.0%) as the least predominant bacteria species. Among the oral site, lower right quadrant showed increase isolation rate of 30(15.0%) bacteria followed by lower left quadrant 23(11.5%) while upper right quadrant accounted 15(7.5 %) with the least isolation rate. There was no statistically significant difference in the prevalence of non-oral bacteria in right quadrant and left quadrant samples from dental disease patients (P < 0.05). Non-oral bacteria isolate exhibited 57.1-100% resistant to Ertapenem, colisitn, amoxillicin, azetronam, colistin, ampicillin and clindamycin with Multiple Antibiotic Resistant Index (MARI) ranged from 0.4-0.7, indicating high level of multi-drug resistance but were susceptible to ciprofloxacin 77.8%, gentamicin 100% and imipenem 100%. Conclusion: The high antibiotic resistant and increase multi-drug resistance outcome reported among non-oral bacteria in this study calls for strengthened efforts in antibiotic stewardship and infection prevention and control measures in dental practices with the need to implement regular awareness programs at time interval to control and manage multi-drug resistance bacteria through judicious use of antibiotic to re-establish dominance over multi-drug resistance non-oral bacteria implicated in dental diseases.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Phenotypic Characterization and Antibiogram of Non-Oral Bacteria Isolates from Patients Attending Dental Clinic at Federal College of Dental Technology and Therapy Medical Center Enugu

Edemekong

Iroha²,

Thompson³

et al. 2022

IJPR

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Detection of FOX-AmpC-β-lactamase gene and antibiogram of AmpC-beta-lactamase-producing pathogens isolated from chronic suppurative otitis media patients in Nigeria

Amadi,

Nchedo,

Thaddaeus Obaji

et al. 2023

IJM

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Background and Objectives: AmpC-producing Gram-negative bacterial (GNB) pathogens are distributed worldwide, espe- cially in clinical settings. This study aimed to determine the antibiogram and the type of AmpC-β-lactamase gene harboured by GNB pathogens implicated in chronic suppurative otitis media (CSOM) cases. Materials and Methods: Ear swab samples (300) collected from patients with active CSOM were analysed using standard microbiological techniques. Phenotypic and molecular detection of AmpC β-lactamase production was done by cefoxitin/ cloxacillin double-disk synergy test and PCR respectively. Antibiogram was determined by disk diffusion technique. Results: Among the GNB pathogens isolated from CSOM patients, P. aeruginosa was the most predominant (36.3%); fol- lowed by K. pneumoniae (22.3%), and E. coli (13.7%). Patients with active CSOM showed increased bacteria isolation rate from bilateral ear discharges than unilateral ear discharges. E. coli and P. aeruginosa were more prevalent among patients with duration of discharge >2 weeks; recording 9.0% and 20.3% respectively. AmpC β-lactamase producers accounted for 14.0%; they were highly resistant (60%-100%) to cephalosporins, trimethoprim-sulfamethoxazole, ofloxacin, amoxicillin, and tetracycline, but very susceptible (70.4%-100%) to ciprofloxacin, imipenem, and amikacin. Multiple antibiotic resistance indices of isolates ranged from 0.7-0.8. FOX-AmpC-β-lactamase gene was detected in 3.9% of the isolates. Conclusion: The detection of AmpC β-lactamase-producing multidrug-resistant GNB pathogens harbouring FOX-AmpC- β-lactamase gene among patients with CSOM infections in our study is a serious public health problem which needs urgent intervention.

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Characterization of fumarate reduction by Klebsiella pneumoniae isolated from patients with chronic periodontitis

Sharma,

Garg,

Hasan

et al. 2023

APJMBB

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Chronic periodontitis is oral inflammatory gum disease which affects the tissues that support the teeth. It is caused by formation of bacterial plaque consisting of bacterial species that produces various virulence factors. Oral bacterial species from the periodontitis patients include species of Fusobacterium, Porphyromonas, Prevotella, Tannerella, etc. In the present study, we isolated the Klebsiella pneumoniae strain 9A from the pockets of patients suffering from chronic periodontitis. The strain was isolated on enriched tryptic soya media containing 10 mM fumarate and identified based on Gram staining, 16S rRNA gene sequencing, biochemical tests and ability to reduce fumarate. Our study is the first report of anaerobic fumarate reduction capabilities of Klebsiella spp. isolated from chronic periodontitis patients, which may help in growth and survival of species in periodontal pockets leading to progression of the disease. In addition, experimental evidence is provided through reduction, growth, and inhibitor assays for the presence of fumarate reductase (Frd) activities. Furthermore, we detected a 400 bp frdA gene fragment in Klebsiella pneumoniae strain 9A using degenerate PCR. This fragment show similarity to fumarate reductases flavoprotein subunit (FrdA). Our findings suggest that the FrdA subunit is responsible for anaerobic fumarate respiration in Klebsiella spp. to be important in the survival of bacteria in oxygen-deprived environments in humans such as periodontal pockets, thus contributing to its pathogenicity.

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Molecular characterization of Enterobacteriacea isolated from gingivitis and periodontitis patients and the antimicrobial activity of mouth wash agents

Cited by 3 publications

References 24 publications

Phenotypic Characterization and Antibiogram of Non-Oral Bacteria Isolates from Patients Attending Dental Clinic at Federal College of Dental Technology and Therapy Medical Center Enugu

Phenotypic Characterization and Antibiogram of Non-Oral Bacteria Isolates from Patients Attending Dental Clinic at Federal College of Dental Technology and Therapy Medical Center Enugu

Detection of FOX-AmpC-β-lactamase gene and antibiogram of AmpC-beta-lactamase-producing pathogens isolated from chronic suppurative otitis media patients in Nigeria

Characterization of fumarate reduction by Klebsiella pneumoniae isolated from patients with chronic periodontitis

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