Molecular characterization of Eimeria species in macropods

Yang, Rongchang; Fenwick, Stan; Potter, Abbey; Elliot, Aileen; Power, Michelle; Beveridge, Ian; Ryan, Una

doi:10.1016/j.exppara.2012.07.003

Cited by 37 publications

(27 citation statements)

References 23 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Eimeria parasites exhibit immense diversity in host range including mammals, birds, reptiles, fish and amphibians [81][82][83][84][85][86]. It is estimated that there are many thousands of Eimeria species [87].…”

Section: Eimeriamentioning

confidence: 99%

Genomics of Apicomplexa

Martínez-Ocampo¹

2018

Farm Animals Diseases, Recent Omic Trends and New Strategies of Treatment

View full text Add to dashboard Cite

Apicomplexa is a eukaryotic phylum of intracellular parasites with more than 6000 species. Some of these single-celled parasites are important pathogens of livestock. At present, 128 genomes of phylum Apicomplexa have been reported in the GenBank database, of which 17 genomes belong to five genera that are pathogens of farm animals: Babesia, Theileria, Eimeria, Neospora and Sarcocystis. These 17 genomes are Babesia bigemina (five chromosomes), Babesia divergens (514 contigs) and Babesia bovis (four chromosomes and one apicoplast); Theileria parva (four chromosomes and one apicoplast), Theileria annulata (four chromosomes), Theileria orientalis (four chromosomes and one apicoplast) and Theileria equi (four chromosomes and one apicoplast); Eimeria brunetti (24,647 contigs), Eimeria necatrix (4667 contigs), Eimeria tenella (12,727 contigs), Eimeria acervulina (4947 contigs), Eimeria maxima (4570 contigs), Eimeria mitis (65,610 contigs) and Eimeria praecox (53,359 contigs); Neospora caninum (14 chromosomes); and Sarcocystis neurona strains SN1 (2862 contigs) and SN3 (3191 contigs). The study of these genomes allows us to understand their mechanisms of pathogenicity and identify genes that encode proteins as a possible vaccine antigen.

show abstract

Section: Eimeriamentioning

confidence: 99%

Genomics of Apicomplexa

Martínez-Ocampo¹

2018

Farm Animals Diseases, Recent Omic Trends and New Strategies of Treatment

View full text Add to dashboard Cite

show abstract

“…In histological tissue sections where parasites were detected, total DNA was extracted from 4-5 slivers of 5 µm sections of selected wax block tissues using a Power Soil DNA Kit (MolBio, Carlsbad, California) with some modifications, as described by Yang et al (2012). Briefly, the tissues for DNA extraction were subjected to four cycles of freeze/ thaw (liquid nitrogen followed by boiling water) to ensure efficient lysis of tissues before being processed using the Power Soil DNA Kit, according to the manufacturer's protocol.…”

Section: Dna Isolationmentioning

confidence: 99%

Observations on the health of Tandanus tropicanus (Teleostei: Plotosidae) from an Australian river system

Kelly¹,

Gibson-Kueh²,

Ebner³

et al. 2016

Australian Zoologist

View full text Add to dashboard Cite

Wild Wet Tropics tandan Tandanus tropicanus were collected from the Bloomfield River, Queensland, for examination by histopathology and bacteriology. This provided an opportunity to establish baseline information on the general health and parasite fauna of native freshwater catfish in a pristine river. Histology of gill tissue revealed epitheliocystis in one fish. This is the first report of epitheliocystis in T. tropicanus. Bacterial culture showed light growth of bacteria from the kidney of only two fish, and these were identified as Aeromonas veronii, A. jandaei and Bacillus/Lactobacillus spp. An unidentified monogenean infection was observed in the gills of four fish, and trematode metacercariae were observed in the extra-ocular tissue of four fish. Nematodes were observed in the tissues of nine fish, and sequence and preliminary phylogenetic analysis of PCR products using an ITS primer suggest that these parasites may be a previously unreported Contracaecum species. benthic habitat. There has been no comprehensive study on the health of any native catfish species in Australia, and therefore there is a lack of baseline reference data on these important freshwater species.As part of a larger study on the health of native catfish in northern Australia, a number of T. tropicanus were collected from the Bloomfield River in northern Queensland. The section of the Bloomfield River in which the catfish were collected is considered to be of high ecological value and effectively unmodified, with no exotic fish species recorded (Burrows 2009; Department of Environment and Resource Management, Queensland Government 2010). This presents a unique opportunity to study the baseline health of a newly described native Australian catfish species (Welsh et al. 2014) in a relatively pristine environment. Materials and Methods SamplingNineteen T. tropicanus were collected from one location on the Bloomfield River (15.9868S, 145.2882E, WGS84) in northern Queensland. Juvenile and adult catfish (6.8-207.8 g and 8-30 cm total length) were collected using single-winged fyke nets set overnight on 9 th and 10 th May, 2014. The catfish were kept in holding nets in the river until they were transported to James Cook University (JCU), Cairns on 11 th May, where they were held in well aerated aquaria, until euthanized immediately before examination on 11 th and 12 th May.Fish were euthanized by being placed into a prolonged anaesthetic bath of isoeugenol (AquiS TM ). Sex, weight and total body length measurements were recorded, and fish examined for any external or internal gross abnormalities. For bacterial isolation, pooled kidney and spleen tissues from each individual were homogenised and inoculated onto blood agar (BA) and Edwardsiella ictaluri medium (EIM) (Shotts and Waltman 1990). Inoculated plates were couriered in sturdy insulated boxes under ambient temperatures to the Animal Health Laboratories, Department of Agriculture and Food Western Australia, Perth, and incubated at 24°C for 48 to 72 hours. Isolates were iden...

show abstract

“…using primers 75 and conditions described by Yang et al, (2012). Amplification at the mitochrondial 76 cytochrome oxidase gene (COI) locus was initially attempted using methods 77 described by Ogedengbe et al, (2011).…”

Section: Pcr Amplification and Sequencing 74mentioning

confidence: 99%