Molecular Characterization of Duck Plague Virus for Determination of TCID&lt;sub&gt;50&lt;/sub&gt;

Jahan, Salim; Rahman, Mizanur; Ahmed, Rony; Kabir, Akma; Oli, ShamsulKaunain; Hassan, Jayedul; Siddique, Mahbubul Pratik; Rahman, Bahanur

doi:10.3329/ralf.v8i1.53274

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…Furthermore, the phylogenetic tree was constructed using the Neighbor-Joining method. The p -distance method was used for evolutionary distance, and the bootstrap value was 1,000 [ 13 ]. The nucleotide sequence generated in this study has been deposited in the GenBank.…”

Section: Methodsmentioning

confidence: 99%

First report of Aliarcobacter cryaerophilus in ready-to-cook chicken meat samples from super shops in Bangladesh

Mahmud¹,

Kabir²,

Hossain³

et al. 2023

J Adv Vet Anim Res

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Objective: This study aimed to isolate Aliarcobacter cryaerophilus in ready-to-cook poultry meat in Bangladesh. Materials and Methods: Thirty drumstick samples were collected from super shops in Dhaka city (n = 10), Mymensingh city (n = 10), and Patuakhali town (n = 10). After sample processing, they were cultured in Blood agar media with Campylobacter base using a microfilter (0.42 nm). Suspected colonies were subjected to DNA extraction and PCR assay targeting 16SrRNA genes. Then, sequencing was performed for confirmation. Results: Of 30 samples, 3 (10%) were positive for A. cryaerophilus. Phylogenetic analysis shows that our isolate has strong similarities with one of the isolates from China. Conclusion: The presence of this organism in ready-to-cook poultry meat is a significant concern for consumers as it bears zoonotic importance.

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Section: Methodsmentioning

confidence: 99%

First report of Aliarcobacter cryaerophilus in ready-to-cook chicken meat samples from super shops in Bangladesh

Mahmud¹,

Kabir²,

Hossain³

et al. 2023

J Adv Vet Anim Res

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“…The study was conducted from July 2021 to December 2022. Rectal swabs were collected using a cotton apparatus and placed in 1ml of phosphate buffer saline (PBS) The samples were then stored at 4˚C until transported to the Department of Microbiology and Hygiene, Bangladesh Agricultural University [29]. After PLOS ONE centrifugation at 10,000 RPM for 10 minutes, a 200ul supernatant was collected and stored at -80 ˚C until further processing.…”

Section: Sample and Data Collectionmentioning

confidence: 99%

Epidemiology and molecular characterization of Feline panleukopenia virus from suspected domestic cats in selected Bangladesh regions

Kabir,

Habib,

Chouhan

et al. 2023

PLoS ONE

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Feline panleukopenia (FPL) is a highly contagious cat disease and is endemic in Bangladesh. The study aims to describe the epidemiology and molecular characterization of the Feline panleukopenia virus from the suspected domestic cats in selected Bangladesh regions. Randomly, 161 rectal swabs were collected from the pet hospitals between July 2021 and December 2022. A structured questionnaire was administered through face-to-face interviews with cat owners in order to collect data on potential risk factors for FPL, such as age, sex, sharing litter boxes and every day utensils in multicat households, vaccination history, hospital visits for other diseases, and season. The rectal swabs were tested by PCR targeting the VP2 capsid protein gene, and six PCR-positive samples were further sequenced for molecular characterizations. The risk factors for FPLV were identified using multivariable logistic regression analysis. The overall prevalence of FPL among suspects was 22.9%. The mortality and case fatality were 10.6%, and 45.9%, respectively. However, mortality in kittens was significantly higher (16.4%) than younger cats. The odds of FPL were 8.83 times (95% CI: 3.14–24.85) higher among unvaccinated cats than vaccinated cats. The winter season had almost six times (95% CI: 1.38–24.40) higher odds of FPL than rainy season. In a multicat house, the odds of FPL was about five times (95% CI: 1.93–13.45) higher for cats that shared a litter box and food utensils compared to those that did not engage in such sharing. Visiting hospitals for other reasons nearly triples the odds of FPL (OR: 2.80, 95% CI: 1.04–7.54) compared to cats that do not visit hospitals. Analysis of partial sequence of the VP2 gene revealed genetic variations among the isolates from different regions. Among these isolates, four were identical to FPLV isolates from South Korea and China, while one showed complete homology with FPLV isolates from Thailand. In contrast, the remaining one was 100% identical to Carnivore protoparvovirus-1 isolated from a feline sample in Italy. Our isolates were classified into three distinct clades alongside Feline panleukopenia virus and Carnivore protoparvovirus-1. One in every three suspected cats was infected with Feline panleukopenia. Regular vaccination of the cats, especially those that share common litter box and food utensils and visit hospitals for other purposes, will help reduce the prevalence of FPL in Bangladesh. Besides, it is worth emphasizing the existence of genetic diversity among the circulating Feline panleukopenia viruses in Bangladesh.

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Molecular Characterization of Duck Plague Virus for Determination of TCID<sub>50</sub>

Cited by 2 publications

References 26 publications

First report of Aliarcobacter cryaerophilus in ready-to-cook chicken meat samples from super shops in Bangladesh

First report of Aliarcobacter cryaerophilus in ready-to-cook chicken meat samples from super shops in Bangladesh

Epidemiology and molecular characterization of Feline panleukopenia virus from suspected domestic cats in selected Bangladesh regions

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