Molecular Characterization of Drug Resistance in Hepatitis B Viruses Isolated from Patients with Chronical Infection in Turkey

Aşan, Ali; Sayan, Murat; Akhan, Sıla; Koruk, Süda Tekin; Aygen, Bilgehan; Sırmatel, Fatma; Eraksoy, Haluk; Tuna, Nazan; Köse, Şükran; Kaya, Ali Hakan; Tülek, Necla; Demir, Nazlım Aktuğ; Mıstık, Reşit; Örmen, Bahar; Korkmaz, Fatime; Yıldırmak, Taner; Ural, Onur; Aydın, Mehtap; Turgut, Hüseyin; Günal, Özgür; Demirtürk, Neşe

doi:10.5812/hepatmon.12472

Cited by 3 publications

(3 citation statements)

References 41 publications

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“…The target region and amino acid position for the determination of HBV pol gene mutation were as follows: overlapping surface gene segments 100, 101, 105, 109, 110, 114, 117-121, 123, 124, 126, 127, 128-135, 137, 139, 140-142, 144-149, 151-153, 155-157, 161, 172, 173, 175, 176, and 193-196. The following target region and amino acid position for the determination of HBV pol gene mutation were analyzed: rt gene segments 74, 80, 82, 84, 85, 139, 149, 156, 169, 173, 180, 181, 184, 194, 200, 202, 204, 214, 215, 233, 236, 237, 238, and 250 (Sayan et al 2012;Asan et al 2018).…”

Section: Methodsmentioning

confidence: 99%

“…The ADAPVEM status has not yet been known for the TRNC, these results are initial data for monitoring of such mutations in the future. (Sayan et al 2013;Asan et al 2018;Ozguler and Sayan 2018).…”

Section: Mutation Characteristicmentioning

confidence: 99%

“…Sequences obtained were subsequently analyzed using a special online tool, the Geno2pheno (Centre of Advanced European Studies and Research, Bonn, Germany) drug resistance platform. The following target region and amino acid positions were analyzed for the determination of antiviral drug-associated potential vaccine-escape mutations (ADAPVEM) regions 161, 164, 172, 173, 175, 176, 182, and 193-196; HBIg selected escape mutation regions 118, 120, 123, 124, 129, 133, 134, 144, and 145; vaccine escape mutation regions 120, 126, 133, 143-145, and 193; Hepatits B misdiagnosis mutation regions 120, 131, 133, and 143; immuneselected mutation regions 100, 101, 105, 109, 110, 114, 117, 119, 120, 123, 127, 128, 130-134, 140, and 143-145. The target region and amino acid position for the determination of HBV pol gene mutation were as follows: overlapping surface gene segments 100, 101, 105, 109, 110, 114, 117-121, 123, 124, 126, 127, 128-135, 137, 139, 140-142, 144-149, 151-153, 155-157, 161, 172, 173, 175, 176, and 193-196. The following target region and amino acid position for the determination of HBV pol gene mutation were analyzed: rt gene segments 74, 80, 82, 84, 85, 139, 149, 156, 169, 173, 180, 181, 184, 194, 200, 202, 204, 214, 215, 233, 236, 237, 238, and 250 (Sayan et al 2012;Asan et al 2018).…”

Section: Molecular Characteristics Of Hepatitis B Virus 4 451mentioning

confidence: 99%

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Molecular Epidemiology of Hepatitis B Virus in Turkish Cypriot

Sumer

Sayan

2019

Polish Journal of Microbiology

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There is an increased demand for molecular and epidemiological information regarding Hepatitis B Virus (HBV) infection as the disease severity depends on these specifications. We have aimed to analyze nucleos(t)ide analogues (NA) resistance and typical HBsAg escape mutations with the dispersion of HBV genotype/subgenotype/HBsAg serotypes on overlapping pol/S gene regions in the Turkish population. Samples were collected in Northern Cyprus. Reverse transcriptase (rt) region between 80–250 amino acids were amplified. Typical HBsAg escape mutations were determined as HBIg escape (6.48%), vaccine escape (8.34%), HBsAg misdiagnosis (9.25%), and immune escape mutations (8.34%). NAs resistances were determined as primary (2.78%), partial (2.78%), and compensatory mutations (26.85%) in overlapping pol/S gene region. The study patients were predominantly infected with HBV genotype D/D1 (98%). However, the predominant HBsAg serotype was ayw2 (99%). The most common NA resistance mutation was rtQ215H/P/S (16.67%), however, for S gene the misdiagnosis mutations were observed most frequently (9.25%). We can conclude that HBV D/D1 is the dominant strain and ayw2 is the dominant serotype in the Turkish Cypriot. Cyprus is an island located in the Eastern Mediterranean region, and it is, therefore, a key location for human trafficking and immigration; as a result of this reputation, it is necessary to analyze HBV phylogenetically for local dynamics, and our results indicate that treatment naïve population is prone to these pol/S gene mutations. However, if HBV strains were also analyzed among Greek Cypriots too, this would enable a complete island survey. With this work, we believe that we have enlightened this subject for further research.

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Section: Methodsmentioning

confidence: 99%

Section: Mutation Characteristicmentioning

confidence: 99%

Section: Molecular Characteristics Of Hepatitis B Virus 4 451mentioning

confidence: 99%

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Molecular Epidemiology of Hepatitis B Virus in Turkish Cypriot

Sumer

Sayan

2019

Polish Journal of Microbiology

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Molecular characterization of hepatitis B virus reveals circulation of multiple subgenotypes of genotype D with clinically important mutations in central India

Shivlata

Pacholi²,

Chouksey³

et al. 2021

Indian Journal of Medical Microbiology

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Evaluation of the pol/S Gene Overlapping Mutations in Chronic Hepatitis B Patients in Northern Cyprus

Arıkan

Sayan

Şanlıdağ

et al. 2019

Polish Journal of Microbiology

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Mutations associated with the pol and the S gene can emerge as a consequence of the high replication capacity and proofreading deficiencies of hepatitis B virus during replication. The current study was constructed to evaluate primary, partial, compensatory and the escape mutations in chronic hepatitis B patients in Northern Cyprus. The samples of HBsAg positive treatment naïve 100 patients were involved in this study. HBV pol gene region was sequenced, amplified and HBV pol/S gene mutations were determined. The samples of thirty-two patients were excluded because of their low viral load (HBV < 1000 ıu/ml). Among the sequenced 68 samples, there was a partial mutation (1.5%) and 36.7% displayed a resistance profile to lamivudine, adevofir, and telbivudine. Immune response escape, vaccine escape, HBIg and diagnosis escape mutations were determined in 24%, 10%, 6%, and 4% samples of the patients, respectively. Additionally, there were six different combined mutations. These data underscored that there is no concern for primary mutations in Northern Cyprus, however, we have identified a compensatory mutation (rtV173M) that may have primary mutation characteristics by combining with other mutation patterns. Additionally, HBsAg escape mutants demonstrated that detection of the S gene together with the pol gene mutations might be beneficial and important to monitor the surveillance of S variants.

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Molecular Characterization of Drug Resistance in Hepatitis B Viruses Isolated from Patients with Chronical Infection in Turkey

Cited by 3 publications

References 41 publications

Molecular Epidemiology of Hepatitis B Virus in Turkish Cypriot

Molecular Epidemiology of Hepatitis B Virus in Turkish Cypriot

Molecular characterization of hepatitis B virus reveals circulation of multiple subgenotypes of genotype D with clinically important mutations in central India

Evaluation of the pol/S Gene Overlapping Mutations in Chronic Hepatitis B Patients in Northern Cyprus

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