Molecular characterization of Ditylenchus dipsaci on garlic in Turkey

Abstract

Garlic plant, which has an important place in the world economy as well as in human nutrition, is grown extensively in Turkey. The stem and bulb nematode, Ditylenchus dipsaci (Kühn) is one of the most important biotic stresses and is under quarantine as it significantly interrupts garlic production wherever it emerges. Thus, being up to date regarding the distribution and population of the stem and bulb nematode, which is locally found in garlic cultivation areas, identification of the races found on the garlic plant at the molecular level and knowing the host spectrum of the races found at the region are of great importance. The objective of this study is to perform species diagnosis using the species-specific molecular markers at ITS-rDNA regions of the stem and bulb nematode isolates collected from the garlic cultivation areas in Turkey in 2016 and 2017. In the study, molecular screening data of the nematodes isolated from the plant and soil samples were analyzed phylogenetically. In this study, upon molecular screening using 9 different species-specific SSR and SCAR primers, it was found that 34 nematode samples of 53 are D. dipsaci. Nematode samples were from the garlic fields in Kastamonu,

Bazı yerel sarımsak genotip ve köy çeşitlerinin soğan sak nematoduna, Ditylenchus dipsaci (Kühn, 1857) Filipjev, 1936 (Rhabditida: Anguinidae) karşı dayanıklılıklarının belirlenmesi

“…KAS-9 population of D. dipsaci which was isolated from a garlic field in Kastamonu Province, Taşköprü District, Vakıfbelören Village (N: 41°30'07.37", E: 34°15'01.19", Elevation: 650 m) in Türkiye, and identified by morphological and molecular methods (Ates Sonmezoglu et al, 2020), was used in the study. KAS-9 population was multiplied on sterile carrot discs to obtain nematode inoculums.…”

“…Ditylenchus dipsaci has been detected in garlic-growing areas in 80 different countries in all the continents except Antarctica to date (EPPO, 2022). Ditylenchus dipsaci was found in the garlic growing areas of Tekirdağ and Kırklareli provinces in the Marmara Region; Kastamonu, Tokat and Amasya provinces in the Black Sea Region; Kahramanmaraş province in the Mediterranean Region; Balıkesir and Bursa provinces in the Aegean Region; Gaziantep, Hatay and Adıyaman provinces in the Southeastern Anatolia Region; Aksaray province in the Central Anatolia Region in Türkiye (Ates Sonmezoglu et al, 2020;Öcal, 2021).…”

Determination of the host status of some plant species with four different garlic populations of Ditylenchus dipsaci (Kühn, 1857) Filipjev, 1936 (Tylenchida: Anguinidae)

“…Ditylenchus dipsaci populations were collected in 2017 and 2018 from Adıyaman, Gaziantep, Kahramanmaraş and Kastamonu Provinces, Turkey, in areas with intensive garlic production. Nematode populations were identified as D. dipsaci (Ates Sonmezoglu et al, 2020). Location information about D. dipsaci populations is given in Table 1.…”