Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy

Resch, Yvonne; Weghofer, Margit; Seiberler, Susanne; Horak, Friedrich; Scheiblhofer, Sandra; Linhart, Birgit; Swoboda, Ines; Thomas, Wayne R.; Thalhamer, Josef; Valenta, Rudolf; Vrtala, Susanne

doi:10.1111/j.1365-2222.2011.03798.x

Cited by 91 publications

(86 citation statements)

References 39 publications

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“…Sensitization rates to Der p 10 between 10 and 18% in Austria up to 55% in Zimbabwe were reported in the literature [9][10][11]. As Austria directly borders to the southern Bavarian part of Germany and eating habits with mainly meat and less seafood diet is common, we expected a comparable sensitization to tropomyosin of above 10%.…”

Section: Discussionmentioning

confidence: 56%

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Tropomyosin sensitization in house dust mite allergic patients

Becker

Gröger

Canis

et al. 2011

Eur Arch Otorhinolaryngol

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The growing popularity and frequency of consumption of seafood is accompanied by an increasing number of adverse reactions reported in literature. Allergic reactions to seafood can generate a variety of symptoms ranging from a mild oral allergy syndrome to keen anaphylactic reactions. Tropomyosin, the major shellfish allergen is regarded to be responsible for clinical cross-reactivity to inhaled house dust mites. The aim of the study was to investigate the prevalence of sensitization to tropomyosin in house dust mite allergic patients in southern Bavaria and to compare the results with allergic symptoms. Sera of house dust mite allergic patients (positive skin prick test, allergen-specific IgE and intranasal provocation) were screened for IgE antibodies to tropomyosin (Der p 10). Patients were contacted by phone to evaluate allergic symptoms when consuming seafood. IgE antibodies to house dust mite tropomyosin (Der p 10) could be found in 4 out of 93 sera (4.3%). Two of these four patients (50%) showed itching and swelling of oral mucosa accompanied by bronchial obstruction after consumption of shrimp. Two patients had no problems when eating seafood. None of the seronegative patients complained about any health problems during or after consumption of seafood. In conclusion, cross-reactivity to tropomyosin in house dust mite allergic patients in southern Bavaria, Germany is rarer than suspected. Beside the direct allergic reactions, a further part of reactions to seafood must therefore be ascribed to other mechanisms such as intoxication or intolerance to, e.g. additives in the food product.

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Section: Discussionmentioning

confidence: 56%

“…Weghofer et al [9] found sensitization rates of 9% in France, 10% in Italy and 18% in Austria and Sweden. Comparable results for Austria of 15% could be shown by Resch et al [10]. Westritschnig et al analyzed sensitization proWles of African people in Zimbabwe and compared it with data from central Europe.…”

Section: Introductionmentioning

confidence: 80%

Tropomyosin sensitization in house dust mite allergic patients

Becker

Gröger

Canis

et al. 2011

Eur Arch Otorhinolaryngol

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“…mite bodies or mite culture) [19,32]. The contents of Der p 1 and 2 have been well investigated in allergen extracts [21,32], but no studies have been performed to investigate mite extracts regarding other important allergens such as Der p 5, 7, 10 and 21 [5,25,26,34,35]. We found that certain of these allergens were absent in 8 of the 10 extracts studied.…”

Section: Discussionmentioning

confidence: 84%

“…The recombinant allergens rDer p 5, 7, 10 and 21 were expressed in the vector pET 17b as nonfusion proteins [ [5,25,26 ]and Casset and Vrtala, unpubl.]. rDer p 2 was expressed in the vector pET 17b with a C-terminal hexahistidine tag [27] and an rDer p 14 fragment (aa 1–260) was expressed in the vector pET 19b with an N-terminal hexahistidine tag [28].…”

Section: Methodsmentioning

confidence: 99%

Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial <b><i>Dermatophagoides pteronyssinus</i></b> Extracts

Casset¹,

Mari

Purohit

et al. 2012

Int Arch Allergy Immunol

159

112

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Background: Diagnosis and immunotherapy of house-dust mite (HDM) allergy is still based on natural allergen extracts. The aim of this study was to analyze commercially available Dermatophagoides pteronyssinus extracts from different manufacturers regarding allergen composition and content and whether variations may affect their allergenic activity. Methods: Antibodies specific for several D. pteronyssinus allergens (Der p 1, 2, 5, 7, 10 and 21) were used to analyze extracts from 10 different manufacturers by immunoblotting. Sandwich ELISAs were used to quantify Der p 1 and Der p 2 in the extracts. Mite-allergic patients (n = 45) were skin-tested with the extracts and tested for immunoglobulin E (IgE) reactivity to a panel of 10 mite allergens (Der p 1, 2, 4, 5, 7, 8, 10, 14, 20 and 21) by dot blot. Results: Only Der p 1 and Der p 2 were detected in all extracts but their concentrations and ratios showed high variability (Der p 1: 6.0–40.8 µg ml^–1; Der p 2: 1.7–45.0 µg ml^–1). At least 1 out of 4 allergens (i.e. Der p 5, 7, 10 and 21) was not detected in 8 of the studied extracts. Mite-allergic subjects showed different IgE reactivity profiles to the individual mite allergens, the extracts showed different allergenic activity in skin-prick tests and false-negative results. Conclusions: Commercially available D. pteronyssinus extracts lack important allergens, show great variability regarding allergen composition and content and some gave false-negative diagnostic test results in certain patients.

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“…Further profiling of allergen-binding patterns, as Simpson et al [34] suggest, could be informative within their newly defined risk categories. It has been shown that the 15% of European HDM-allergic subjects with IgE to the tropomyosin Der p 10 bind more HDM allergens [35], so ongoing investigations are warranted. With the spread of HDM allergy into developing regions [36], patterns of sensitisation that are not adequately represented by current HDM extracts could emerge.…”

Section: Discussionmentioning

confidence: 99%

Quantitation of IgE Binding to the Chitinase and Chitinase-Like House Dust Mite Allergens Der p 15 and Der p 18 Compared to the Major and Mid-Range Allergens

Hales

Elliot

Chai

et al. 2012

Int Arch Allergy Immunol

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Background: The prevalence of IgE binding to the group 15 and 18 house dust mite (HDM) allergens of the Dermatophagoides species is reported to be >50% and they are the major allergens of HDM-sensitised dogs. The objective was to quantitate the IgE titres to Der p 15 and Der p 18 and evaluate their importance in human HDM sensitisation compared to the known major and mid-tier allergens. Methods: Der p 15 and Der p 18 were produced in Pichia pastoris, and their structure validated by circular dichroism. IgE binding was measured in 37 Australian HDM-allergic adults using a quantitative DELFIA™ assay. Results: The prevalence of IgE titres to Der p 15 and Der p 18 >0.1 ng/ml was low (38%) and only one subject had a titre >10 ng/ml to either allergen. The mean anti-Der p 15 and Der p 18 titres were 1.2 and 2.6 ng/ml, respectively, i.e. approximately 10- to 20-fold lower than the response to the major Der p 1 and Der p 2 allergens (p < 0.001). The IgE responses to Der p 15 and Der p 18 were lower than the mid-tier allergens Der p 5 and Der p 7 and although they correlated with each other, they did not correlate with titres to either the major or mid-tier allergens. Conclusions: Sensitisation to Der p 15 and Der p 18 makes a minor contribution to anti-HDM IgE titres, and the titres do not correlate with the size of the response to the major allergens.

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Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy

Abstract: SummaryBackground-Tropomyosins represent clinically relevant seafood allergens but the role of mite tropomyosin, Der p 10, in house dust mite (HDM) allergy has not been studied in detail.

Cited by 91 publications

References 39 publications

Tropomyosin sensitization in house dust mite allergic patients

Tropomyosin sensitization in house dust mite allergic patients

Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial <b><i>Dermatophagoides pteronyssinus</i></b> Extracts

Quantitation of IgE Binding to the Chitinase and Chitinase-Like House Dust Mite Allergens Der p 15 and Der p 18 Compared to the Major and Mid-Range Allergens

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