Molecular characterization of Cytochrome B gene in indigenous pig

Saikia, J.; Roy, T. C.; Naskar, S.; Das, Bula; Ferdoci, A M

doi:10.5958/0976-0555.2015.00092.8

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…The aim of this study to was to carry out sequence analyses of the mtDNA COI gene regions of the Androctonus crassicauda, Buthacus macrocentrus, and Scorpio maurus species, and thus to determine the phylogenetic relationship and genetic variation among these species. Mitochondrial DNA has been largely used to carry out phylogenetic studies in different animal species (Saikia et. al., 2015).…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial cytochrome oxidase subunit I-sequence variation in three scorpion species from southeast Turkey

Toprak

Parmaksız

Aslan

2018

IJAR

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Southeastern Anatolia is the richest region in Turkey in terms of scorpion fauna. In this region, a number of studies on the fauna of scorpions, as well as epidemiology and clinical findings of scorpion stings in terms of public health, have been carried out, but there is no study on the phylogenetic relationships between species. In this study, 18 individuals of three species were collected from five locations. Sequence analysis was conducted by amplification of a 546 base region from part of mtDNA COI region. Six polymorphic regions and four haplotypes were determined for A. crassicauda, a species important for humans and mean haplotype and nucleotide variations were 0.602 and 0.00555 respectively. Sequences from all individuals were subjected to the blast tool of the NCBI database and assessed with close species, so a phylogenetic tree was formed. On the tree, samples from Şanlıurfa and Diyarbakır were on different branches while the sample from Iran (HM567333) was on a branch from the other two. The species that was the closest to Scorpio maurus from Batman (KF953944) was the sample from Diyarbakır. Because there was no other sequence for Buthacus macrocentrus, it was located close to the Buthacus sp. (KF548116) sample. As a result, the locations of sample species were determined as a result of phylogenetic analyses. A n d r o c t o n u s c r a s s i c a u d a -7 ( S a n li u r f a ) A n d r o c t o n u s c r a s s i c a u d a -1 0 ( S a n l iu r f a ) A n d r o c t o n u s c r a s s i c a u d a -5 ( S a n li u r f a ) A n d r o c t o n u s c r a s s i c a u d a -4 ( S a n li u r f a ) A n d r o c t o n u s c r a s s i c a u d a -3 ( S a n li u r f a ) A n d r o c t o n u s c r a s s i c a u d a -2 ( S a n li u r f a ) A n d r o c t o n u s c r a s s i c a u d a -1 ( S a n li u r f a ) A n d r o c t o n u s c r a s s i c a u d a -1 8 ( S a n l iu r f a ) A n d r o c t o n u s c r a s s ic a u d a -2 6 ( S a n l i u r f a ) A n d r o c t o n u s c r a s s ic a u d a -6 ( S a n l iu r f a ) A n d r o c t o n u s c r a s s ic a u d a -1 3 ( D i y a r b a k ir ) A n d r o c t o n u s c r a s s ic a u d a -1 6 ( D iy a r b a k i r ) A n d r o c t o n u s c r a s s ic a u d a -2 8 ( D i y a r b a k ir ) H M 5 6 7 3 3 3 . 1 A n d r o c t o n u s c r a s s i c a u d a K J 5 3 8 3 9 7 . 1 A n d r o c t o n u s a u s t r a l is J N 0 1 8 1 5 0 . 1 A n d r o c t o n u s h o g g a r e n s i s K J 5 3 8 2 7 9 . 1 A n d r o c t o n u s a m o r e u x i K J 5 3 8 2 5 5 . 1 A n d r o c t o n u s m a u r i t a n i c u s K J 5 3 8 2 0 8 .

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Section: Introductionmentioning

confidence: 99%

Mitochondrial cytochrome oxidase subunit I-sequence variation in three scorpion species from southeast Turkey

Toprak

Parmaksız

Aslan

2018

IJAR

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“…The same findings were reported by Koju et al, (2017) when t h ey r epor t ed t he phylogeny of living pikas (Ochotonidae, Ochotona) from China by using two mitochondrial (CYT B and COI) and five nuclear gene segments (RAG1,RAG2,TTN,OXAIL and IL1RAPL1). Mitochondrial DNA in animals causes the five to ten times greater rate of evolution than in nuclear genome, that is why it is hypothesized to be ideal marker to study divergence between domestic and wild animals (Saikia et al, 2015). In the following study, diversity among the India peafowls at government and private sectors was investigated by sequence analysis of cytochrome b gene to elucidate the genetic structure and degree of differentiation between the birds at different locations.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Phylogenetic analysis based studies on genetic variation of Cytochrome b gene of Indian peafowl (Pavo cristatus) in Pakistan

Ng’ambi

Ramuthaga

Brown

2017

IJAR

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This study was designed to analyze genetic variation between Indian peafowl available at different locations by targeting the Cytochrome b gene. A total of ten birds (n=10) were selected randomly for sample collection. Five birds were selected from Government sector and five from private breeding farms. DNA was extracted, purified and measured by using Nano drop. Extracted DNA was amplified using universal primers targeting Cytochrome b gene on polymerase chain reaction (PCR). PCR product was run on a gel for the desired DNA bands. DNA from gel was eluted and sent for sequencing. The sequences were compared with a reference reported sequence of cyt b gene of Indian peafowl with Accession No. L08379.1to find out the genetic diversity. Indian peafowl of government sector showed more similarity 95% rather than bird of private sector with 90% homology with reference Accession No. More genetic variation, which is the guarantee of resistance to disease and environmental fitness among the Indian peafowl at private sector, might be due to random reproductive behavior.

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