Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil

Abstract

The study was conducted on 25 properties of the settlements São José I and Salvador, located in the municipalities of Brejo Alegre and Birigui, in the State of São Paulo, Brazil. A record of variables was elaborated and included data such as gender, breed and age of the animals. A total of 231 stool samples were collected from bovines aged one to six months, 128 being females and 103 males, 131 crossbred and 100 Holstein. Among the 231 samples, 17 (7.36%) were positive for Cryptosporidium spp. both by malachite green negative staining and by nested-PCR. Of the 17 positive samples, 14 were sequenced in agarose gel. These sequences were detected between 99% and 100% of genetic similarity for the following species. One sequence was similar to C. parvum (AB513880.1), one to C. bovis (MF074602.1), two to C. ryanae (KT922233.1), one to C. felis (KM977642.1) and nine were similar for C. andersoni reference MF350628. C. andersoni was found in animals aged 2–6 months, an age group which is different from those described by several authors. The presence of C. parvum indicates that the calves in the studied region should be considered a potential source for zoonotic transmission. For the first time to our knowledge, C. felis was identified in cattle in America.

Evaluation of fecal smear methods for research on Cryptosporidium spp. oocysts in the feces of dairy calves

“…Regarding the age group and species found, the animals identified with C. parvum were between one and two months old, corroborating the data found by Santín et al (2004), Fayer et al (2007, and Matos et al (2019). C. ryanae was identified in three samples from animals between two and four months of age, similar to the findings of Matos et al (2019).…”