Molecular characterization of corneal ulcers causing Staphylococcus aureus

Abstract

Background: The human eye is one of the most remarkable sensory systems. Human beings gather most of the information about the external environment through their eyes and thus rely on sight more than on any other sense, with the eye being the most sensitive organ we have. Molecular characterization of Staphylococcus aureus from the cases of corneal ulcers.Methods: A total of 300 samples of corneal ulcers collected from various ophthalmology hospitals, government hospital and clinical laboratories of different places of Maharashtra, India. The Isolates were identified based on the colony morphology and biochemical reaction. The isolates were subjected for antibiotic sensitivity test and perform its molecular characterization.Results: In present study, 39 coagulase positive Staphylococcus aureus, pathogenic bacteria isolated from corneal ulcers.Conclusions: Staphylococcus aureus is one of the most significant pathogens in bacterial keratitis. Early diagnosis and prompt treatment are needed to minimize the possibility of permanent vision loss and reduce structural damage to the cornea.

Pseudomonas gessardii—A novel pathogenic bacterium associated with the cases of corneal ulcers and producing virulent pyoverdine pigment

“…A regular procedure was followed for the collection of samples, such as Bard-Parker surgical blade for corneal scratches and broth moistened cotton swabs for rubbing the lid margin of the infected cornea [2]. Specimens were transferred into containers with nutrient broth and were transported directly to the laboratory.…”

“…A total of 300 samples were collected from the Government Medical College (GMC), ophthalmic hospitals, and clinical laboratories of the several districts and cities of Maharashtra, India. The hospitals were selected based on the sample availability, geographical proximity, and feasibility of conducting the study [2,6,10].…”

“…The human eye is a sensory organ. The vision depends on the clarity of the cornea [1,2]. Corneal ulcer is considered to be the primary causative agent of loss of vision (10%) [2,3].…”

“…The vision depends on the clarity of the cornea [1,2]. Corneal ulcer is considered to be the primary causative agent of loss of vision (10%) [2,3]. "Corneal ulcer is defined as the loss of corneal layers due to infection and formation of raw, scoop out area [4].…”

“…Tears and corneal scratches pave way for the entrance of bacteria resulting in an infection. Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Enterococci, Pseudomonas, Klebsiella, Proteus, Bacillus cereus, and many other bacteria are reported to cause corneal ulcers [2,5,[7][8][9][10].…”