Molecular Characterization of Community- and Healthcare-Associated Methicillin-Resistant <i>Staphylococcus aureus</i> Isolates in Southern Taiwan

Lee, Tai-Min; Yang, Ming‐Chang; Yang, Tzu-Feng; Lee, Pei-Ling; Chien, Hsin-I; Hsueh, Jui-Chen; Chang, S. H.; Hsu, Chao-Hsun; Chien, Shang‐Tao

doi:10.1089/mdr.2015.0020

Cited by 10 publications

(8 citation statements)

References 32 publications

(28 reference statements)

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“…According to the previous reports [ 18 ], HA-MRSA is usually detected with SCC mec type I, II, and III; in contrast, CA-MRSA is reported as carrying type IV and V. In this study, SCC mec III was the dominant type in HA-MRSA strains (87.3 %), also the main type of CA-MRSA (60.9 %). This study confirms that CA-MRSA clones partially carry the SCC mec type III and SCC mec type I, as reported in a recent study in Guangzhou [ 16 ].…”

Section: Discussionsupporting

confidence: 55%

“…SCC mec typing of MRSA strains was conducted by multiplex PCR method as previously described [ 13 ]. The genetic relationship of the isolates was determined by PFGE as previously described [ 18 ]. Cluster analysis was performed with the software program BioNumerics 5.0 (Maths, Belgium) using the Dice coefficient and the unweighted pair group method (UPGMA).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular epidemiology and characteristic of virulence gene of community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolates in Sun Yat-sen Memorial hospital, Guangzhou, Southern China

et al. 2016

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of both hospital and community infections globally. It's important to illuminate the differences between community-acquired MRSA (CA-MRSA) and hospital-acquired MRSA (HA-MRSA), but there have been confusions on the definition, especially for the MRSA isolates identified within 48 h of admission. This study aimed to determine the molecular characteristics and virulence genes profile of CA and HA-MRSA isolates identified less than 48 h after hospital admission in our region. Methods: A total 62 MRSA isolates identified within 48 h after admission and the clinical data were collected. Antimicrobial susceptibility test (AST) of collected isolates were performed according to the guidelines of Clinical and Laboratory Standards Institute (CLSI) 2015, and staphylococcal cassette chromosome mec (SCCmec) typing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) and virulence gene profiling were performed to explore the molecular diversity. Results: SCCmec III and sequence type (ST) 239 were the most prevalent SCCmec type and ST in both CA and HA-MRSA groups. HA-MRSA group had higher prevalence of SCCmec III (87.2 %) and ST239 (79.5 %) compared with CA-MRSA (60.9 and 43.4 %, both P < 0.001), while the frequency of SCCmec IV (26.0 %) and ST59 (21.7 %) were higher in CA-MRSA than its counterpart (P < 0.001 and P = 0.003). MRSA-ST239-III was the predominant type in this study (61.3 %, 38/62), especially in HA-MRSA group (76.9 %, 30/39). However, CA-MRSA strains exhibited more diversity in genotypes in this study. Meanwhile, CA-MRSA tended to have lower resistant percentage to non-β-lactams antibiotics but more virulence genes carriage, especially the staphylococcal enterotoxins (SE) genes. Notably, seb gene was only detected in CA-MRSA isolates (52.2 %), likely a significant marker for CA-MRSA isolates. Panton-Valentine leukocidin gene (PVL) was highly detected in both groups, while appeared no significantly different between CA-MRSA (47.8 %) and HA-MRSA (43.6 %). Conclusions: Our findings support a difference in the molecular epidemiology and virulence genes profile of CA-MRSA and HA-MRSA. Furthermore, this study indicates a possible transmission from HA-MRSA to CA-MRSA, which may cause the overlap of the definition.

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Section: Discussionsupporting

confidence: 55%

Section: Methodsmentioning

confidence: 99%

Molecular epidemiology and characteristic of virulence gene of community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolates in Sun Yat-sen Memorial hospital, Guangzhou, Southern China

et al. 2016

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“…Another striking finding in this study was that 11 CA‐MRSA (47.8%) and 7 HA‐MRSA (35%) harboured the gene for PVL toxin (Table ). Although PVL is known as a common virulence factor of CA‐MRSA and HA‐MRSA strains seldom carry this gene, several studies in Taiwan (Lee et al., ) and USA (Zetola, Francis, Nuermberger, & Bishai, ) showed that their HA‐MRSA isolates had a considerable rate of PVL positivity in their regions supporting the hypothesis that PVL is not the sole marker for recognizing a community lifestyle, but rather one of several important factors that may play a key role in the successful dissemination of CA‐MRSA (Zhang, McClure, Elsayed, Tan, & Conly, ). Our above findings confirm that the CA‐ and HA‐MRSA classifications are no longer distinct and the boundaries between HA‐ and CA‐MRSA are becoming increasingly difficult.…”

Section: Discussionmentioning

confidence: 96%

What is behind phylogenetic analysis of hospital‐, community‐ and livestock‐associated methicillin‐resistantStaphylococcus aureus?

El-Hamid

Bendary

Merwad

et al. 2019

Transbound Emerg Dis

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Summary Methicillin‐resistant Staphylococcus aureus (MRSA) has been shown to be the predominant life‐threatening pathogen in Egypt. MRSA is a major cause of severe healthcare‐associated (HA) infections. During the last decades, the incidence of community‐associated (CA) MRSA infections has a complex epidemiology arising from the circulation of different strains in the general population. Moreover, livestock‐associated (LA) MRSA emerged recently becomes an emerging threat to public health. Therefore, it is important to illuminate the differences between CA‐, HA‐ and LA‐MRSA to shed light on their genetic diversity and evolution. This study presents the first data on analysing the correlation between CA‐, LA‐ and HA‐MRSA using antibiogram typing, molecular characteristics and antimicrobial resistance and virulence genes’ profiles. Overall, HA‐MRSA strains tended to be multidrug resistant and less virulent than both LA‐ and CA‐MRSA strains. Importantly, CA‐MRSA strains had a high homology with each of HA‐ and LA‐MRSA. However, no similarity was observed between HA‐ and LA‐MRSA. Our findings suggest that the epidemiological changes in genetic behaviour between HA‐ and LA‐MRSA are due to the presence of CA‐MRSA confirming that CA‐MRSA has created a public health crisis worldwide.

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“…Some recent studies report SCCmec type III as the most frequent type isolated from hospital-acquired infections. This can be explained as the studies conducted on the MRSA strains mostly isolated from nosocomial infections, as the present study [12,13].…”

Section: Discussionmentioning

confidence: 77%

“…De Oliveira et al [12] reported the most frequent cassette as SCCmec type II with a rate of 53.7% amongst 123 Staphylococcus aureus strains. Lee et al [13] found the most frequent type as type I (8.7%) amongst the community-acquired cases, and as type V (24.4%) amongst the hospital-acquired isolates.…”

Section: Discussionmentioning

confidence: 97%

Determination of the staphylococcal cassette chromosome in methicillin-resistant Staphylococcus aureus strains isolated from various clinical samples

Aysegül¹,

Rukiye²,

Sandallı³

et al. 2016

Russ. Open Med. J.

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Abstract:Aim -The present study aimed to detect mecA and staphylococcal cassette chromosome mec (SCCmec) types in methicillinresistant Staphylococcus aureus (MRSA) isolates obtained from various clinical samples in two university hospitals. It was also aimed to make comparison amongst the isolates. Materials and Methods -A total of 99 MRSA strains isolated from various clinical samples between 2011-2015 were included in the study. Bacterial deoxyribonucleic acid (DNA) was extracted from Staphylococcus aureus strains using GF-1 DNA extraction Kit (Vivantis, Malaysia). mecA gene were detected, and SCCmec cassette types were determined by multiplex polymerase chain reaction (PCR) first, and following specific PCR. Specific MRSA strains such as COL type I, PER3 type Ia, and HU25 type IIIa were used as the quality control strains for optimization of multiplex PCR. The amplification products were electrophoresed using agarose gel electrophoresis in TAE buffer (mixture of tris base, acetic acid and ethylenediaminetetraacetic acid). Results -mecA gene was detected in 60 Staphylococcus aureus isolates, and these were identificated as MRSA. Amongst the MRSA strains, SCCmec type III was the most frequent cassette type (42 isolates, 70.0%). SCCmec type I was detected in 27 isolates (45.0%), type II was in 26 isolates (43.3%), and type V in 23 isolates (38.3%). Conclusion -In the present study, the most frequent cassette was detected as SCCmec type III in concordance with the studies conducted in Turkey and in some regions in the world. In conclusion, determination of epidemiological and molecular characteristics of MRSA strains has critical importance because of the difficulties in the treatment and of the nosocomial infections and epidemics they caused. The data obtained would contribute to the preventions in terms of epidemiology.

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Molecular Characterization of Community- and Healthcare-Associated Methicillin-Resistant Staphylococcus aureus Isolates in Southern Taiwan

Cited by 10 publications

References 32 publications

Molecular epidemiology and characteristic of virulence gene of community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolates in Sun Yat-sen Memorial hospital, Guangzhou, Southern China

Molecular epidemiology and characteristic of virulence gene of community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolates in Sun Yat-sen Memorial hospital, Guangzhou, Southern China

What is behind phylogenetic analysis of hospital‐, community‐ and livestock‐associated methicillin‐resistantStaphylococcus aureus?

Determination of the staphylococcal cassette chromosome in methicillin-resistant Staphylococcus aureus strains isolated from various clinical samples

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