Molecular Characterization of Common Olive Varieties in Israel and the West Bank Using Randomly Amplified Polymorphic DNA (RAPD) Markers

Wiesman, Zeev; Avidan, N.; Lavee, S.; Quebedeaux, Bruno

doi:10.21273/jashs.123.5.837

Cited by 71 publications

(48 citation statements)

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“…The high level of polymorphism observed in this study for all three marker systems is consistent with results from previous studies carried out on olive cultivars by means of different molecular markers (Fabbri et al 1995;Wiesman et al 1998;Angiolillo et al 1999;Baldoni et al 2000;Rallo et al 2000;Belaj et al 2001;Besnard et al 2001;Sanz-CortØs et al 2001), thereby confirming the great diversity within the cultivated olive germplasm (Bartolini et al 1998).…”

Section: Discussionsupporting

confidence: 92%

Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive

et al. 2003

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RAPDs, AFLPs and SSRs were compared in terms of their informativeness and efficiency in a study of genetic diversity and relationships among 32 olive cultivars cultivated in Italy and Spain. SSRs presented a higher level of polymorphism and a greater information content, as assessed by the expected heterozygosity, than AFLPs and RAPDs. The lowest values of expected heterozygosity were obtained for AFLPs, which, nevertheless were the most efficient marker system due to their capacity to reveal the highest number of bands per reaction and because of the high values achieved for a considerable number of indexes. All three techniques discriminated the genotypes very effectively, but only SSRs were able to discriminate the cultivars Frantoio and Cellina. The correlation coefficients of similarity were statistically significant for all three marker systems used but were lower for the SSR data than for RAPDs and AFLPs. For all markers a high similarity in dendrogram topologies was obtained although some differences were observed. All the dendrograms, including that obtained by the combined use of all the marker data, reflect some relationships for most of the cultivars according to their geographic diffusion. AMOVA analysis detected greater genetic differentiation among cultivars within each country than it did between the two countries.

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Section: Discussionsupporting

confidence: 92%

Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive

et al. 2003

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“…In olive, RAPD (Wiesman et al 1998;Mekuria et al 1999;Belaj et al 2001), AFLP (Angiolillo et al 1999), RFLP (Besnard et al 2000) and simple sequence repeat (SSR) markers Cipriani et al 2002) have been used for genetic diversity studies and cultivar identification, however, to date, there is almost no information available on segregation analysis of genes or molecular makers in an olive cross population. We report the construction of a first olive linkage map using a cross between two highly heterozygous olive cultivars (Leccino and Dolce Agogia) and employing dominant and codominant markers in a pseudo test-cross strategy.…”

Section: Introductionmentioning

confidence: 99%

A first linkage map of olive (Olea europaea L.) cultivars using RAPD, AFLP, RFLP and SSR markers

Rosell

Angiolillo²,

Guerrero³

et al. 2003

Theor Appl Genet

134

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The first linkage map of the olive (Olea europaea L.) genome has been constructed using random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphisms (AFLP) as dominant markers and a few restriction fragment length polymorphisms (RFLP) and simple-sequence repeats (SSR) as codominant markers. Ninety-five individuals of a cross progeny derived from two highly heterozygous olive cultivars, Leccino and Dolce Agogia, were used by applying the pseudo test-cross strategy. From 61 RAPD primers 279 markers were obtained - 158 were scored for Leccino and 121 for Dolce Agogia. Twenty-one AFLP primer combinations gave 304 useful markers - 160 heterozygous in Leccino and 144 heterozygous in Dolce Agogia. In the Leccino map 249 markers (110 RAPD, 127 AFLP, 8 RFLP and 3 SSR) were linked. This resulted in 22 major linkage groups and 17 minor groups with fewer than four markers. In the Dolce Agogia map, 236 markers (93 RAPD, 133 AFLP, 6 RFLP and 4 SSR) were linked; 27 major linkage groups and three minor groups were obtained. Codominant RFLPs and SSRs, as well as few RAPDs in heteroduplex configuration, were used to establish homologies between linkage groups of both parents. The total distance covered was 2,765 cM and 2,445 cM in the Leccino and Dolce Agogia maps, respectively. The mean map distance between adjacent markers was 13.2 cM in Leccino and 11.9 cM in Dolce Agogia, respectively. Both AFLP and RAPD markers were homogeneously distributed in all of the linkage groups reported. The stearoyl-ACP desaturase gene was mapped on linkage group 4 of cv. Leccino.

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“…RAPD molecular markers have revealed two types of evidence: one indicates that the olive cultivars have no special relationship (Belaj et al 2001), while the other, which arises with the selection of cultivars with a certain geographic relationship, show that the differentiation of the olive cultivars follows a criterion strongly influenced by edaphoclimatic characteristics (Wiesman et al 1998, Gonzalo-Claros et al 2000. In our study, the distances between the accessions enable the grouping of the Portuguese cultivars that lack a clear correlation with their geographic origin (Fig.…”

Section: Resultsmentioning

confidence: 55%

Genetic diversity assessment in Portugal accessions of Olea europaea by RAPD markers

et al. 2008

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Eighty seven olive (Olea europaea ssp. sativa L.) cultivar accessions from Portugal were characterized by means of randomly amplified polymorphic DNA (RAPD) markers. Of the 11 arbitrary 10-mer primers tested a total of 92 polymorphic bands were obtained, representing 87.6 % of the total amplification products. Twenty nine different genotypes were clearly discriminated. Differences were not found among the amplification profiles from different individuals of the same cultivar. All the genotypes could be identified by the combination of three primers: OPR-1, OPK-14 and OPA-1, seven genotype-specific markers being detected. Genetic relationships were estimated by the unweighted pair-group method with arithmetic averaging (UPGMA). The genetic analysis of the results showed a gradual distance between the various cultivars, making it difficult to identify well-differentiated phylogenetic groups, although two clusters were distinguishable with 35 % similarity, in addition to three independent branches with lower similarity: Galega, Tentilheira and Redondal. The dendrogram reflect some relationships for most of the cultivars according to the use of the fruit and ecological adaptation.

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Molecular Characterization of Common Olive Varieties in Israel and the West Bank Using Randomly Amplified Polymorphic DNA (RAPD) Markers

Cited by 71 publications

References 0 publications

Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive

Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive

A first linkage map of olive (Olea europaea L.) cultivars using RAPD, AFLP, RFLP and SSR markers

Genetic diversity assessment in Portugal accessions of Olea europaea by RAPD markers

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